Callus induction and plant regeneration in Sinopodophyllum hexandrum

Key words: 
• botany /
• Sinopodophyllum hexandrum /
• tissue culture /
• callus /
• adventitious buds /
• browning

Abstract: To protect and exploit the endangered medicinal plant Sinopodophyllum hexandrum，a rapid regeneration system of tissue cultue was established. The effects of cytokinins ［thidiazuron （TDZ） and 6-benzylaminopurine （6-BA）］ and auxins ［naphthalene acetic acid （NAA） and indole-3-acetic acid （IAA）］ on callus induction, adventitious bud induction, and rooting were studied. Using mature embryos of Sinopodophyllum hexandrum as explants Murashige and Skoog medium （MS） + 0.5 mgL－1 TDZ was used to induced embryogenic callus from primary callus so that embryogenic callus developed into globular embryoid and heart-stage embryoid. Results showed differences in percentages for mediums causing callus induction for S. hexandrum with TDZ concentrations of 0.5 mgL－1 TDZ and 500 mgL－1 casein hydrolysate （CH） having the best callus induction rate of 97.8%. To avoid brownness of callus a medium of 160 mgL－1 glutathione （GSH） was used. The optimum medium for adventitious bud differentiation and proliferation was MS + 1.5 mgL－1 6-BA + 0.2 mgL－1 NAA + 500 mgL－1 CH with the highest induction rate of 53.3% and 5.2 multiple shoots. Two treatments of 0.5mgL－1 NAA and 0.5 mgL－1 IAA induced roots, but the most suitable medium was MS + 0.5 mgL－1 IAA having a rooting rate of 22.4%. The rapid propagation system of Sinopodophyllum hexandrum has been preliminarily established, which provide theory foundation for biotechnology breeding and technical guidance for large-scale production ［Ch, 1 fig. 3 tab. 13 ref.］