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HAO Yanmin, CHEN Keli, FENG Lijun, LI Feifei, CUI Minlong, PIAO Chunlan. Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210651
Citation: HAO Yanmin, CHEN Keli, FENG Lijun, LI Feifei, CUI Minlong, PIAO Chunlan. Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210651

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Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris

doi: 10.11833/j.issn.2095-0756.20210651
  • Received Date: 2021-09-14
  • Accepted Date: 2022-03-25
  • Rev Recd Date: 2022-03-15
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris

doi: 10.11833/j.issn.2095-0756.20210651

Abstract:   Objective  Floral organ development is an important factor affecting the ornamental value of flowers, and APETALA1(AP1) genes regulate the formation of floral organs. This study aims to explore the important role of SvAP1 gene of Senecio vulgaris (Asteraceae) in floral organ formation, so as to reveal the regulatory mechanism of the complex inflorescence structure in Asteraceae.   Method  SvAP1 gene was cloned from S. vulgaris. The function of SvAP1 gene was predicted and analyzed by multiple sequence alignment, phylogenetic tree construction, qRT-PCR, overexpression vector construction, and histological staining observation.  Result  The open reading frame (ORF) of SvAP1 gene was 705 bp in length, and encoded 234 amino acids. Multiple sequence alignment and phylogenetic analysis showed that SvAP1 gene belonged to AP1 subfamily of MADS-box gene, and the C-terminus had a conserved motif of paleoAP1. Tissue specific expression analysis of S. vulgaris showed that SvAP1 gene was expressed in both vegetative organs and inflorescences. Morphological observation and paraffin section analysis of transgenic Solanum nigrum showed that compared with wild S. nigrum, the pistil development of transgenic S. nigrum was abnormal, which was characterized by enlarged ovary and increased pistil-like tissue.  Conclusion  The overexpression of SvAP1 gene in S. nigrum affects the pistil development, which is different from the effect of over expression of class A gene in ABC model on floral organ development, that is, the stamens of transgenic S. nigrum have no obvious changes and the pistils aren’t transformed into sepal or leaf-like organs, which may be related to the complexity of the floral organ regulation mechanism and the inflorescence structure of S. vulgaris. In conclusion, SvAP1 gene may play an important role in floral organ formation as a characteristic gene of floral organ. [Ch, 6 fig. 1 tab. 35 ref.]

HAO Yanmin, CHEN Keli, FENG Lijun, LI Feifei, CUI Minlong, PIAO Chunlan. Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210651
Citation: HAO Yanmin, CHEN Keli, FENG Lijun, LI Feifei, CUI Minlong, PIAO Chunlan. Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210651
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