The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems

QIAOGui-rong; LUANWei-jiang; PANHong-wei; ZHUO Ren-ying

Volume 24 Issue 2

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(2): 140-144

QIAOGui-rong, LUANWei-jiang, PANHong-wei, et al. The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems[J]. Journal of Zhejiang A&F University, 2007, 24(2): 140-144.

Citation:

QIAOGui-rong, LUANWei-jiang, PANHong-wei, et al. The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems[J]. Journal of Zhejiang A&F University, 2007, 24(2): 140-144.

The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems

1.
The Research Institute of Subtropical Forestry, the Chinese Academy of Forestry, Fuyang 311400, Zhejiang,China

Funds:

浙江省科学技术攻关项目(2004C32002);国家林业局948项目(2005-4-58)

Received Date: 2006-05-29
Rev Recd Date: 2006-10-27
Publish Date: 2007-04-20

Abstract

It is incompatible between production and need of timber, the aim of the test is breeding fast-growing,super-quality and large-diameter tree cultivar. The LEAFY gene affects plant flowering. Using polymerase chain reaction (PCR) with the primers 5-CACCTCAGAGAGAGAAAAATAGATTATGGATCC-3' and 5- GTCGACAGTTCGGGGGGGAAATATAAT-3', a conservation sequence of the LEAFY gene for Liquidambar formosana(Chinese sweetgum)was cloned. An RNA interference(RNAi)pBIN vector of the LEAFY gene was constructed byGateway technology, and then agrobacteriummediationwas used to transfer the aimgene into the the first two to three leaves of Chinese sweetgum seedlings.Then the transgenic leaveswere cultured to plants in vitro.These plants leaveswere analyzed with PCR and Southern blotting. It is sure that the foreign gene was integratedinto the genome, resulting in four transgenic plants. However, the transgenic four plants development and timber quality has not been analyzed as they are so young. The study is carried on.[Ch, 5 fig. 12 ref.]
- forest tree breeding,
- RNA interference(RNAi),
- Liquidambar formosana(Chinese sweetgum),
- LEAFY RNAi transgenic plants

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems

1. The Research Institute of Subtropical Forestry, the Chinese Academy of Forestry, Fuyang 311400, Zhejiang,China

Funds:

浙江省科学技术攻关项目(2004C32002);国家林业局948项目(2005-4-58)

Received Date: 2006-05-29

Rev Recd Date: 2006-10-27

Publish Date: 2007-04-20

Key words:

forest tree breeding /
RNA interference(RNAi) /
Liquidambar formosana(Chinese sweetgum) /
LEAFY RNAi transgenic plants

Abstract: It is incompatible between production and need of timber, the aim of the test is breeding fast-growing,super-quality and large-diameter tree cultivar. The LEAFY gene affects plant flowering. Using polymerase chain reaction (PCR) with the primers 5-CACCTCAGAGAGAGAAAAATAGATTATGGATCC-3' and 5- GTCGACAGTTCGGGGGGGAAATATAAT-3', a conservation sequence of the LEAFY gene for Liquidambar formosana(Chinese sweetgum)was cloned. An RNA interference(RNAi)pBIN vector of the LEAFY gene was constructed byGateway technology, and then agrobacteriummediationwas used to transfer the aimgene into the the first two to three leaves of Chinese sweetgum seedlings.Then the transgenic leaveswere cultured to plants in vitro.These plants leaveswere analyzed with PCR and Southern blotting. It is sure that the foreign gene was integratedinto the genome, resulting in four transgenic plants. However, the transgenic four plants development and timber quality has not been analyzed as they are so young. The study is carried on.[Ch, 5 fig. 12 ref.]

The LEAFY gene in RNA interference(RNAi)transgenic Liquidambar formosana mediated by Agrobacterium tumefaciems

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views