An inter simple sequence repeats(ISSR)reaction system for<em> Lycoris</em>(Amaryllidaceae)

ZHANG Lei-fan; GAO Yan-hui; ZHUYu-qiu; LIUZhi-gao; TONG Zai-kang; HUANG Hua-hong

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Volume 24 Issue 2

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(2): 156-161

ZHANG Lei-fan, GAO Yan-hui, ZHUYu-qiu, LIUZhi-gao, TONG Zai-kang, HUANG Hua-hong. An inter simple sequence repeats(ISSR)reaction system for Lycoris(Amaryllidaceae)[J]. Journal of Zhejiang A&F University, 2007, 24(2): 156-161.

Citation:

ZHANG Lei-fan, GAO Yan-hui, ZHUYu-qiu, LIUZhi-gao, TONG Zai-kang, HUANG Hua-hong. An inter simple sequence repeats(ISSR)reaction system for Lycoris(Amaryllidaceae)[J]. Journal of Zhejiang A&F University, 2007, 24(2): 156-161.

An inter simple sequence repeats(ISSR)reaction system for Lycoris(Amaryllidaceae)

1.
School of Forestry and Biotechnology, Zhejiang Forestry College, Linan 311300, Zhejiang, China

Funds:

浙江省科学技术攻关项目(2005C32029)

Received Date: 2006-06-20
Rev Recd Date: 2006-10-10
Publish Date: 2007-04-20

Abstract

To study the genetic diversity of Lycoris Herb.(Amaryllidaceae), an orthogonal design was used to optimize an inter simple sequence repeats(ISSR) amplification system on Lycoris using five factors(Mg2+,deoxyribonucleotide triphosphate(dNTP), primers, DNA template, and Taq polymerase) at four levels of concentrations respectively. A suitable ISSR reaction system was constructed with the 20L reaction system containing 1 polymerase chain reaction(PCR)buffer,2.5 mmolL- 1 MgCl2,150molL- 1 dNTP,0.5molL- 1 primer, 50 ng DNA template, and 25.05 nkat Taq DNA polymerase. According to the gradient PCR, the optimal annealing temperature for the ISSR reaction was 57C.[Ch, 7 fig. 2 tab. 23 ref.]
- botany,
- Lycoris,
- intersimple sequence repeats(ISSR),
- orthogonal design,
- polymerase chain reaction(PCR)system

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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An inter simple sequence repeats(ISSR)reaction system for Lycoris(Amaryllidaceae)

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Linan 311300, Zhejiang, China

Funds:

浙江省科学技术攻关项目(2005C32029)

Received Date: 2006-06-20
Rev Recd Date: 2006-10-10
Publish Date: 2007-04-20

Key words:

Abstract: To study the genetic diversity of Lycoris Herb.(Amaryllidaceae), an orthogonal design was used to optimize an inter simple sequence repeats(ISSR) amplification system on Lycoris using five factors(Mg2+,deoxyribonucleotide triphosphate(dNTP), primers, DNA template, and Taq polymerase) at four levels of concentrations respectively. A suitable ISSR reaction system was constructed with the 20L reaction system containing 1 polymerase chain reaction(PCR)buffer,2.5 mmolL- 1 MgCl2,150molL- 1 dNTP,0.5molL- 1 primer, 50 ng DNA template, and 25.05 nkat Taq DNA polymerase. According to the gradient PCR, the optimal annealing temperature for the ISSR reaction was 57C.[Ch, 7 fig. 2 tab. 23 ref.]

Citation:

ZHANG Lei-fan, GAO Yan-hui, ZHUYu-qiu, LIUZhi-gao, TONG Zai-kang, HUANG Hua-hong. An inter simple sequence repeats(ISSR)reaction system for Lycoris(Amaryllidaceae)[J]. Journal of Zhejiang A&F University, 2007, 24(2): 156-161.

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