RAPD analysis of 16 orange varieties in Lishui of Zhejiang

ZENG Yan-ru; HU Ying; SI Jin-ping; XU Xiang-hua

Volume 22 Issue 5

Feb. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2005 > 22(5): 481-485

ZENG Yan-ru, HU Ying, SI Jin-ping, XU Xiang-hua. RAPD analysis of 16 orange varieties in Lishui of Zhejiang[J]. Journal of Zhejiang A&F University, 2005, 22(5): 481-485.

Citation:

ZENG Yan-ru, HU Ying, SI Jin-ping, XU Xiang-hua. RAPD analysis of 16 orange varieties in Lishui of Zhejiang[J]. Journal of Zhejiang A&F University, 2005, 22(5): 481-485.

RAPD analysis of 16 orange varieties in Lishui of Zhejiang

1.
Key Laboratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
2.
Lishui Institute of Forestry Science , Lishui 323000 , Zhejiang , China

Funds:

浙江省科学技术重点项目(2003C2002)

Received Date: 2004-12-09
Rev Recd Date: 2005-04-11
Publish Date: 2005-10-20

Abstract

Sixteen orange varieties cultivated in Lishui of Zhejiang were analyzed for their phylogeny with RAPD markers .Totally 216 RAPD loci were amplified with 21 primers , with 10.29 loci per primer on the average . Polymorphic loci accounted for 96.76 %.For different varieties , 0 -10 bands could be amplified with each primer .Among all the primers used , the primer S13 is the least informative with only 48 bands amplified and S317 most informative with 143 bands amplified .Citrus grandis var. wentanyu is a very special variety , in which 8 out of 21 primers had no bands amplified .It has been found through UPGMA clustering that varieties of Ougan , Ponkan , Unshu Mikan and fruitgrape clustered separately ;Citrus unshiu C .clementina and C .hybrid were close to Unshu Mikan in genetic distance and C .paradis 'Changshanhuyou' to C .sinensis var. brasiliensis . In addition , C .suavissima 'Seedless' could be distinguished from C .suavissima with primer S304 , while C . poonensis 'Seedless' from C .poonensis with primer S356 .It is concluded that the varieties tested here are different from one another in DNA and RAPD markers could be used to distinguish them .[ Ch , 4 fig , 1 tab .8 ref .]
- RAPD,
- orange variety,
- phylogeny,
- Citrus suavissioma 'Seedless',
- Citrus poonensis 'Seedless'

Relative Article

[1]	YE Xiaoling, ZHAO Yuhong, JIANG Nan, ZHANG Min, ZHANG Chi. Fertility effect of E3 ubiquitin ligase gene CsRNF217 in transgenic tobacco plants . Journal of Zhejiang A&F University, 2023, 40(6): 1181-1187. doi: 10.11833/j.issn.2095-0756.20220710
[2]	CHEN Wenchong, JIA Ning, DONG Ang, YAN Daoliang, ZENG Yanru. A protocol for methylation-sensitive amplified polymorphism markers and its application to a methylation analysis in Carya cathayensis . Journal of Zhejiang A&F University, 2019, 36(3): 468-478. doi: 10.11833/j.issn.2095-0756.2019.03.006
[3]	YU Dihu, ZHANG Chi, KE Fuzhi, JING Luyang, GU Xuejiao, WU Baoyu, ZHANG Min. Cloning and expression analysis of CHS gene family in Citrus suavissima 'Seedless' . Journal of Zhejiang A&F University, 2019, 36(5): 943-949. doi: 10.11833/j.issn.2095-0756.2019.05.013
[4]	WANG Weili, HE Liping, YU Minfen, YUAN Huwei, YAN Daoliang, ZHAO Liang, YU Youxiang, LIU Jun, ZHAO Li, ZHENG Bingsong. Phylogenetic relationships among 12 cultivars of Ilex verticillata based on ISSR molecular markers . Journal of Zhejiang A&F University, 2018, 35(4): 612-617. doi: 10.11833/j.issn.2095-0756.2018.04.005
[5]	ZHU Mimi, ZHANG Chi, CHANG Ailing, DANG Wanyu, ZHOU Caihong, YU Dihu, WU Yingying, ZHANG Min. Expression of microsporocyte meiosis with special genes RAD51 and MS1 in Citrus suavissima ‘Seedless’ . Journal of Zhejiang A&F University, 2016, 33(6): 921-927. doi: 10.11833/j.issn.2095-0756.2016.06.001
[6]	ZHANG Min, LIU Zhihui, SONG Xueen, ZHU Mimi, DANG Wanyu, YAN Fuhua, ZHANG Chi. Cloning and expression analysis of CsLTP and CsLOX in seedless Ougan (Citrus suavissima 'Seedless') . Journal of Zhejiang A&F University, 2014, 31(6): 823-830. doi: 10.11833/j.issn.2095-0756.2014.06.001
[7]	LIU Bao, FAN Huihua, PENG Zhuqing, LIU Yan, CHEN Cunji. Molecular polymorphic analysis with geographic provenances of Sapindus mukorossi . Journal of Zhejiang A&F University, 2014, 31(1): 151-155. doi: 10.11833/j.issn.2095-0756.2014.01.023
[8]	FAN Fan-rong. Genetic diversity of endangered Artocarpus hypargyreus . Journal of Zhejiang A&F University, 2010, 27(2): 266-271. doi: 10.11833/j.issn.2095-0756.2010.02.017
[9]	SHEN Jun-ling, NI Hui-qun, CHEN Xiao-yang, HUANG Shao-wei. Genetic diversity of Jatropha curcas with SRAP molecular markers . Journal of Zhejiang A&F University, 2010, 27(3): 347-353. doi: 10.11833/j.issn.2095-0756.2010.03.005
[10]	ZHENG Yong-ping, ZHENG Quan, YU Ji-ying, ZHANG Ying, FAN Wen-feng. Hybridization breeding and AFLP analysis of relative relationship of Dendrobium nobile . Journal of Zhejiang A&F University, 2009, 26(1): 137-141.
[11]	MO Wen-juan, YUAN De-yi, DUAN Jing-hua, ZOU Feng. Simple sequence repeat analysis on pear cultivars in Niitaka line . Journal of Zhejiang A&F University, 2009, 26(5): 639-643.
[12]	ZHANG Jun-hong, LU Yang-hai, LU Yong-quan, ZHANG Jian-ping, SI Jin-ping. Genetic relationships between Prunus salicina ‘Hongjing’ and ther important plum cultivars . Journal of Zhejiang A&F University, 2009, 26(1): 58-62.
[13]	WUZhu-hua, SHI Ji-sen, XI Meng-li, LIUGuang-xin. Pollen characteristics and relationships of 12 species and 6 cultivars of Lilium . Journal of Zhejiang A&F University, 2007, 24(4): 406-412.
[14]	ZENG Yan-ru, ZHANG Lian-bing, SI Jin-ping, XU Xiang-hua. Preliminary study on seedlessness of seedless Ougan . Journal of Zhejiang A&F University, 2005, 22(4): 359-362.
[15]	CHENG Xiao-jian, YANG Ping, LIN Bo-nian, ZHENG Bing-song. Genetic relationship of myrobalan plum, plum and apricot cultivars by RAPD analysis . Journal of Zhejiang A&F University, 2004, 21(1): 40-43.
[16]	LIN Xia, TAO Zheng-ming, ZHANG Qing-liang, HUANG Jian. Cutivating seedling raised from seed of Ficus concinna var .subsessilis . Journal of Zhejiang A&F University, 2003, 20(3): 325-327.
[17]	TANG Juan-juan, FAN Yi-rong, ZHU Mu-yuan. Analysis of the genetic diversity of Pinus taiwanensis populations . Journal of Zhejiang A&F University, 2003, 20(1): 23-26.
[18]	ZHANG Bin, XU Li-yong. Extracting camphor wood oil from camphor tree seeds . Journal of Zhejiang A&F University, 2001, 18(1): 57-59.
[19]	Huang Jianqin. Embryology reasons for lower seed-setting in Liriodendron chinense . Journal of Zhejiang A&F University, 1998, 15(3): 269-273.
[20]	Guan Kanglin, Huang Jianqing, He Fuji. Cone Seed-extracting Percentage and Full Seed Percentage for Seed Orchard of Chinese Fir. . Journal of Zhejiang A&F University, 1997, 14(2): 127-133.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1429) PDF downloads(249) Cited by()

Proportional views

RAPD analysis of 16 orange varieties in Lishui of Zhejiang

1. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. Lishui Institute of Forestry Science , Lishui 323000 , Zhejiang , China

Funds:

浙江省科学技术重点项目(2003C2002)

Received Date: 2004-12-09

Rev Recd Date: 2005-04-11

Publish Date: 2005-10-20

Key words:

Abstract: Sixteen orange varieties cultivated in Lishui of Zhejiang were analyzed for their phylogeny with RAPD markers .Totally 216 RAPD loci were amplified with 21 primers , with 10.29 loci per primer on the average . Polymorphic loci accounted for 96.76 %.For different varieties , 0 -10 bands could be amplified with each primer .Among all the primers used , the primer S13 is the least informative with only 48 bands amplified and S317 most informative with 143 bands amplified .Citrus grandis var. wentanyu is a very special variety , in which 8 out of 21 primers had no bands amplified .It has been found through UPGMA clustering that varieties of Ougan , Ponkan , Unshu Mikan and fruitgrape clustered separately ;Citrus unshiu C .clementina and C .hybrid were close to Unshu Mikan in genetic distance and C .paradis 'Changshanhuyou' to C .sinensis var. brasiliensis . In addition , C .suavissima 'Seedless' could be distinguished from C .suavissima with primer S304 , while C . poonensis 'Seedless' from C .poonensis with primer S356 .It is concluded that the varieties tested here are different from one another in DNA and RAPD markers could be used to distinguish them .[ Ch , 4 fig , 1 tab .8 ref .]

RAPD analysis of 16 orange varieties in Lishui of Zhejiang

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views