RAPD analysis of 16 orange varieties in Lishui of Zhejiang

ZENG Yan-ru; HU Ying; SI Jin-ping; XU Xiang-hua

Volume 22 Issue 5

Feb. 2017

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ZENG Yan-ru, HU Ying, SI Jin-ping, XU Xiang-hua. RAPD analysis of 16 orange varieties in Lishui of Zhejiang[J]. Journal of Zhejiang A&F University, 2005, 22(5): 481-485.

Citation:

ZENG Yan-ru, HU Ying, SI Jin-ping, XU Xiang-hua. RAPD analysis of 16 orange varieties in Lishui of Zhejiang[J]. Journal of Zhejiang A&F University, 2005, 22(5): 481-485.

RAPD analysis of 16 orange varieties in Lishui of Zhejiang

1.
Key Laboratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;
2.
Lishui Institute of Forestry Science , Lishui 323000 , Zhejiang , China

Funds:

浙江省科学技术重点项目(2003C2002)

Received Date: 2004-12-09
Rev Recd Date: 2005-04-11
Publish Date: 2005-10-20

Abstract

Sixteen orange varieties cultivated in Lishui of Zhejiang were analyzed for their phylogeny with RAPD markers .Totally 216 RAPD loci were amplified with 21 primers , with 10.29 loci per primer on the average . Polymorphic loci accounted for 96.76 %.For different varieties , 0 -10 bands could be amplified with each primer .Among all the primers used , the primer S13 is the least informative with only 48 bands amplified and S317 most informative with 143 bands amplified .Citrus grandis var. wentanyu is a very special variety , in which 8 out of 21 primers had no bands amplified .It has been found through UPGMA clustering that varieties of Ougan , Ponkan , Unshu Mikan and fruitgrape clustered separately ;Citrus unshiu C .clementina and C .hybrid were close to Unshu Mikan in genetic distance and C .paradis 'Changshanhuyou' to C .sinensis var. brasiliensis . In addition , C .suavissima 'Seedless' could be distinguished from C .suavissima with primer S304 , while C . poonensis 'Seedless' from C .poonensis with primer S356 .It is concluded that the varieties tested here are different from one another in DNA and RAPD markers could be used to distinguish them .[ Ch , 4 fig , 1 tab .8 ref .]
- RAPD,
- orange variety,
- phylogeny,
- Citrus suavissioma 'Seedless',
- Citrus poonensis 'Seedless'

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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RAPD analysis of 16 orange varieties in Lishui of Zhejiang

1. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province , Zhejiang Forestry College , Lin’an 311300, Zhejiang , China;

2. Lishui Institute of Forestry Science , Lishui 323000 , Zhejiang , China

Funds:

浙江省科学技术重点项目(2003C2002)

Received Date: 2004-12-09

Rev Recd Date: 2005-04-11

Publish Date: 2005-10-20

Key words:

Abstract: Sixteen orange varieties cultivated in Lishui of Zhejiang were analyzed for their phylogeny with RAPD markers .Totally 216 RAPD loci were amplified with 21 primers , with 10.29 loci per primer on the average . Polymorphic loci accounted for 96.76 %.For different varieties , 0 -10 bands could be amplified with each primer .Among all the primers used , the primer S13 is the least informative with only 48 bands amplified and S317 most informative with 143 bands amplified .Citrus grandis var. wentanyu is a very special variety , in which 8 out of 21 primers had no bands amplified .It has been found through UPGMA clustering that varieties of Ougan , Ponkan , Unshu Mikan and fruitgrape clustered separately ;Citrus unshiu C .clementina and C .hybrid were close to Unshu Mikan in genetic distance and C .paradis 'Changshanhuyou' to C .sinensis var. brasiliensis . In addition , C .suavissima 'Seedless' could be distinguished from C .suavissima with primer S304 , while C . poonensis 'Seedless' from C .poonensis with primer S356 .It is concluded that the varieties tested here are different from one another in DNA and RAPD markers could be used to distinguish them .[ Ch , 4 fig , 1 tab .8 ref .]

RAPD analysis of 16 orange varieties in Lishui of Zhejiang

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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