Establishment of gene transformation acceptor system of embryogenic callus in lily

TANG Dong-qin; QIAN Hong-mei; HUANG Dan-feng; TANG Ke-xuan

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Volume 20 Issue 3

Jul. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2003 > 20(3): 273-276

TANG Dong-qin, QIAN Hong-mei, HUANG Dan-feng, TANG Ke-xuan. Establishment of gene transformation acceptor system of embryogenic callus in lily[J]. Journal of Zhejiang A&F University, 2003, 20(3): 273-276.

Citation:

TANG Dong-qin, QIAN Hong-mei, HUANG Dan-feng, TANG Ke-xuan. Establishment of gene transformation acceptor system of embryogenic callus in lily[J]. Journal of Zhejiang A&F University, 2003, 20(3): 273-276.

Establishment of gene transformation acceptor system of embryogenic callus in lily

1.
The Agricultural and Biology School, Shanghai Jiaotong University , Shanghai 201101 , China;
2.
.FUDAN-SJTU-DOTTINGHAM Plant Biotechnology R &D Center , Shanghai 200030 , China

Publish Date: 2003-09-20

Abstract

To establish stable and efficient gene transformation acceptor systems of embryogenic callus in lily (Lilium longiflorum), experiments of the embryogenic callus induction , plant regeneration and the sensibility of embryogenic callus to several antibiotic are conducted with the flower filaments as explants .The optimum medium for induction and proliferation of embryogenic callus of lily is MS plus BA 0.5 mgL-1 and NAA 1.0mgL-1 , and the optimum medium for bud differentiation is MS plus KT 1.0 mgL-1 and NAA 0.2 mgL-1 .The findings of experiment of the sensitivity of antibiotic for embryogenic callus show that the selection concentrations of kannamycin , hygromycin and cefotasimine are 75 mgL-1 , 20 mgL-1 and 250 mgL-1 respectively .[ Ch , 4 tab .10 ref .]
- tissue culture,
- Lilium longiflorum,
- embryogenic callus,
- antibiotics

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Establishment of gene transformation acceptor system of embryogenic callus in lily

1. The Agricultural and Biology School, Shanghai Jiaotong University , Shanghai 201101 , China;

2. .FUDAN-SJTU-DOTTINGHAM Plant Biotechnology R &D Center , Shanghai 200030 , China

Publish Date: 2003-09-20

Key words:

Abstract: To establish stable and efficient gene transformation acceptor systems of embryogenic callus in lily (Lilium longiflorum), experiments of the embryogenic callus induction , plant regeneration and the sensibility of embryogenic callus to several antibiotic are conducted with the flower filaments as explants .The optimum medium for induction and proliferation of embryogenic callus of lily is MS plus BA 0.5 mgL-1 and NAA 1.0mgL-1 , and the optimum medium for bud differentiation is MS plus KT 1.0 mgL-1 and NAA 0.2 mgL-1 .The findings of experiment of the sensitivity of antibiotic for embryogenic callus show that the selection concentrations of kannamycin , hygromycin and cefotasimine are 75 mgL-1 , 20 mgL-1 and 250 mgL-1 respectively .[ Ch , 4 tab .10 ref .]

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Establishment of gene transformation acceptor system of embryogenic callus in lily

1. The Agricultural and Biology School, Shanghai Jiaotong University , Shanghai 201101 , China;

2. .FUDAN-SJTU-DOTTINGHAM Plant Biotechnology R &D Center , Shanghai 200030 , China

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Establishment of gene transformation acceptor system of embryogenic callus in lily

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Establishment of gene transformation acceptor system of embryogenic callus in lily

1. The Agricultural and Biology School, Shanghai Jiaotong University , Shanghai 201101 , China; 2. .FUDAN-SJTU-DOTTINGHAM Plant Biotechnology R &D Center , Shanghai 200030 , China

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1. The Agricultural and Biology School, Shanghai Jiaotong University , Shanghai 201101 , China;

2. .FUDAN-SJTU-DOTTINGHAM Plant Biotechnology R &D Center , Shanghai 200030 , China