Analysis of the genetic distance of<em> Castanea henryi</em> with RAPD markers

LEI Ri-ping; CHEN Hui; XIE Li-guo

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Volume 19 Issue 3

Jul. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2002 > 19(3): 240-243

LEI Ri-ping, CHEN Hui, XIE Li-guo. Analysis of the genetic distance of Castanea henryi with RAPD markers[J]. Journal of Zhejiang A&F University, 2002, 19(3): 240-243.

Citation:

LEI Ri-ping, CHEN Hui, XIE Li-guo. Analysis of the genetic distance of Castanea henryi with RAPD markers[J]. Journal of Zhejiang A&F University, 2002, 19(3): 240-243.

Analysis of the genetic distance of Castanea henryi with RAPD markers

1.
.Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;
2.
Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China;
3.
.Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China

Publish Date: 2002-09-20

Abstract

The genetic distance of 10 Castanea henryi variances is studies by RAPD (Random Amplified Polymorphic DNA)markers in the paper .The RAPD standard procedure is established , too .The length of amplified fragment is between 500 ～ 2 000 bp after PCR (Polymerase Chain Reaction);and similar coefficient on the level of DNA molecule is carried out , when the coefficient is 0.74 , 10 variances is divided into four classes , that is , You Lizi , Man Zhen , Zhong Jianzui , Hou Zuizhen , Hong Zizhen , Da Jianzui , Cai Zhen , Ren Zuili , Wu Zhen , and Jiajie Maozhen .
- Castanea henryi,
- random amplified polymorphic DNA,
- genetic distance

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis of the genetic distance of Castanea henryi with RAPD markers

1. .Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China;

3. .Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China

Publish Date: 2002-09-20

Key words:

Abstract: The genetic distance of 10 Castanea henryi variances is studies by RAPD (Random Amplified Polymorphic DNA)markers in the paper .The RAPD standard procedure is established , too .The length of amplified fragment is between 500 ～ 2 000 bp after PCR (Polymerase Chain Reaction);and similar coefficient on the level of DNA molecule is carried out , when the coefficient is 0.74 , 10 variances is divided into four classes , that is , You Lizi , Man Zhen , Zhong Jianzui , Hou Zuizhen , Hong Zizhen , Da Jianzui , Cai Zhen , Ren Zuili , Wu Zhen , and Jiajie Maozhen .

LEI Ri-ping, CHEN Hui, XIE Li-guo. Analysis of the genetic distance of Castanea henryi with RAPD markers[J]. Journal of Zhejiang A&F University, 2002, 19(3): 240-243.

Citation:

LEI Ri-ping, CHEN Hui, XIE Li-guo. Analysis of the genetic distance of Castanea henryi with RAPD markers[J]. Journal of Zhejiang A&F University, 2002, 19(3): 240-243.

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通讯作者: 陈斌, bchen63@163.com

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Analysis of the genetic distance of Castanea henryi with RAPD markers

1. .Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China;

3. .Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China

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Analysis of the genetic distance of Castanea henryi with RAPD markers

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通讯作者: 陈斌, bchen63@163.com

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Analysis of the genetic distance of Castanea henryi with RAPD markers

1. .Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China; 2. Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China; 3. .Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China

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1. .Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China;

3. .Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China