Fast propagation techniques of <em>Beltilla ochracea</em> by means of tissue cultures

ZHU Yu-qiu; WANG Xue-gen

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Volume 16 Issue 2

Mar. 2019

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Article Navigation > Journal of Zhejiang A&F University > 1999 > 16(2): 164-169

ZHU Yu-qiu, WANG Xue-gen. Fast propagation techniques of Beltilla ochracea by means of tissue cultures[J]. Journal of Zhejiang A&F University, 1999, 16(2): 164-169.

Citation:

ZHU Yu-qiu, WANG Xue-gen. Fast propagation techniques of Beltilla ochracea by means of tissue cultures[J]. Journal of Zhejiang A&F University, 1999, 16(2): 164-169.

Fast propagation techniques of Beltilla ochracea by means of tissue cultures

ZHU Yu-qiu^1
,,
WANG Xue-gen²

1.
Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;
2.
Jincheng Forest Station of Lin’an City , Lin’an 311300, China

Received Date: 1998-09-28
Rev Recd Date: 1999-01-20
Publish Date: 1999-06-20

Abstract

A study of techniques for tissue cultures of Bletilla ochracea was made in terms of basic culture media , hormones and their proportions and the cutting of original bulbs .Its showed that the medium compose of 1/2MS +1.0 mgL-1BA +0.1 mgL-1NAA was favorable to the proliferation of the original bulb , with 4.21 times as many as thd original proliferated in 60 days ;the medium consisting of Kyoto +1.0 mgL-1BA +0.1mgL-1NAA was favorable to the induction of the original bulb to proliferate and differentiate and the growth of seedlings , with 4.79 times as many as the number of buds of the original bulb differentiated and formed within 60 days ;and the medium with Kyoto +2.0 mgL-1BA +0.1 mgL-1NAA involved had a good effect on the bud induction of pieces cut from the original bulb .In the culture of subsequent generations , vertical cutting of the bulb or natural separation of the original bulb should be used .Some suggestions for further studies are put forward meanwhile .
- Beltilla ochracea,
- tissue cultures,
- protocorm propagation,
- differentiation (biology ),

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Fast propagation techniques of Beltilla ochracea by means of tissue cultures

ZHU Yu-qiu^1,,
WANG Xue-gen²

1. Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;

2. Jincheng Forest Station of Lin’an City , Lin’an 311300, China

Received Date: 1998-09-28
Rev Recd Date: 1999-01-20
Publish Date: 1999-06-20

Key words:

Abstract: A study of techniques for tissue cultures of Bletilla ochracea was made in terms of basic culture media , hormones and their proportions and the cutting of original bulbs .Its showed that the medium compose of 1/2MS +1.0 mgL-1BA +0.1 mgL-1NAA was favorable to the proliferation of the original bulb , with 4.21 times as many as thd original proliferated in 60 days ;the medium consisting of Kyoto +1.0 mgL-1BA +0.1mgL-1NAA was favorable to the induction of the original bulb to proliferate and differentiate and the growth of seedlings , with 4.79 times as many as the number of buds of the original bulb differentiated and formed within 60 days ;and the medium with Kyoto +2.0 mgL-1BA +0.1 mgL-1NAA involved had a good effect on the bud induction of pieces cut from the original bulb .In the culture of subsequent generations , vertical cutting of the bulb or natural separation of the original bulb should be used .Some suggestions for further studies are put forward meanwhile .

ZHU Yu-qiu, WANG Xue-gen. Fast propagation techniques of Beltilla ochracea by means of tissue cultures[J]. Journal of Zhejiang A&F University, 1999, 16(2): 164-169.

Citation:

ZHU Yu-qiu, WANG Xue-gen. Fast propagation techniques of Beltilla ochracea by means of tissue cultures[J]. Journal of Zhejiang A&F University, 1999, 16(2): 164-169.

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Fast propagation techniques of Beltilla ochracea by means of tissue cultures

1. Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;

2. Jincheng Forest Station of Lin’an City , Lin’an 311300, China

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Fast propagation techniques of Beltilla ochracea by means of tissue cultures

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Fast propagation techniques of Beltilla ochracea by means of tissue cultures

1. Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China; 2. Jincheng Forest Station of Lin’an City , Lin’an 311300, China

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1. Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;

2. Jincheng Forest Station of Lin’an City , Lin’an 311300, China