Volume 25 Issue 4
Aug.  2008
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GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.
Citation: GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.

An AFLP analysis system for Carya cathayensis

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  • Corresponding author: ZENG Yan-ru
  • Publish Date: 2008-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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An AFLP analysis system for Carya cathayensis

    Corresponding author: ZENG Yan-ru

Abstract: Total DNA was extracted from young,fully opened leaves of Carya cathayensis(hickory) by an improved cetyl trimethylammonium bromide(CTAB) method and a CTAB-silicon method,based on which an amplified fragment length polymorphism(AFLP)analysis system for hickory was developed. An electrophoresis was used on the amplified products in a 6% denatured polyacrylamide gel,followed by silver staining,with clearly identified bands shown. Specifically:(1)the improved CTAB method was appropriate for DNA extraction from hickory leaves with extracted DNA qualified for AFLP analysis;(2)with a combi-nation of EcoRⅠand MseⅠfor enzyme cutting,the results were the same for the one-step method combining enzyme cutting and adaptor ligation and the two-step method which separated them with the one-step method being much easier;and (3)there were four primer pairs suitable for this AFLP analysis,namely E-ACT + M-CAT,E-ACG + M-CAT,E-ACG + M-CAT,and E-ACG + M-ATG. [Ch,6 fig. 3 tab. 17 ref.]

GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.
Citation: GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.

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