An AFLP analysis system for <EM>Carya cathayensis</EM>

GUO Jin-jian; CHI Wei; ZHAO Xin-zhu; WANG Li; ZENG Yan-ru; ZHANG Qiu-yue

Volume 25 Issue 4

Aug. 2008

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(4): 532-537

GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.

Citation:

GUO Jin-jian, CHI Wei, ZHAO Xin-zhu, WANG Li, ZENG Yan-ru, ZHANG Qiu-yue. An AFLP analysis system for Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 532-537.

An AFLP analysis system for Carya cathayensis

1.
The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;
2.
Changle Co. Ltd，Zhejiang World Trade Center，Lin’an 311300，Zhejiang，China;
3.
Tianze Science and Technology Development Co. Ltd. for Hickory，Zhejiang Forestry College，Lin’an 311300，Zhejiang， China

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Corresponding author: ZENG Yan-ru
Publish Date: 2008-08-20

Abstract

Total DNA was extracted from young，fully opened leaves of Carya cathayensis（hickory） by an improved cetyl trimethylammonium bromide（CTAB） method and a CTAB-silicon method，based on which an amplified fragment length polymorphism（AFLP）analysis system for hickory was developed. An electrophoresis was used on the amplified products in a 6% denatured polyacrylamide gel，followed by silver staining，with clearly identified bands shown. Specifically：（1）the improved CTAB method was appropriate for DNA extraction from hickory leaves with extracted DNA qualified for AFLP analysis；（2）with a combi-nation of EcoRⅠand MseⅠfor enzyme cutting，the results were the same for the one-step method combining enzyme cutting and adaptor ligation and the two-step method which separated them with the one-step method being much easier；and （3）there were four primer pairs suitable for this AFLP analysis，namely E-ACT + M-CAT，E-ACG + M-CAT，E-ACG + M-CAT，and E-ACG + M-ATG. ［Ch，6 fig. 3 tab. 17 ref.］
- botany,
- Carya cathayensis（hickory）,
- extraction of DNA,
- amplified fragment length polymorphism （AFLP）

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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An AFLP analysis system for Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. Changle Co. Ltd，Zhejiang World Trade Center，Lin’an 311300，Zhejiang，China;

3. Tianze Science and Technology Development Co. Ltd. for Hickory，Zhejiang Forestry College，Lin’an 311300，Zhejiang， China

Corresponding author: ZENG Yan-ru

Publish Date: 2008-08-20

Key words:

botany /
Carya cathayensis（hickory） /
extraction of DNA /
amplified fragment length polymorphism （AFLP）

Abstract: Total DNA was extracted from young，fully opened leaves of Carya cathayensis（hickory） by an improved cetyl trimethylammonium bromide（CTAB） method and a CTAB-silicon method，based on which an amplified fragment length polymorphism（AFLP）analysis system for hickory was developed. An electrophoresis was used on the amplified products in a 6% denatured polyacrylamide gel，followed by silver staining，with clearly identified bands shown. Specifically：（1）the improved CTAB method was appropriate for DNA extraction from hickory leaves with extracted DNA qualified for AFLP analysis；（2）with a combi-nation of EcoRⅠand MseⅠfor enzyme cutting，the results were the same for the one-step method combining enzyme cutting and adaptor ligation and the two-step method which separated them with the one-step method being much easier；and （3）there were four primer pairs suitable for this AFLP analysis，namely E-ACT + M-CAT，E-ACG + M-CAT，E-ACG + M-CAT，and E-ACG + M-ATG. ［Ch，6 fig. 3 tab. 17 ref.］

An AFLP analysis system for Carya cathayensis

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