Crude toxin production of <EM>Botryosphaeria dothidea</EM>：initial conditions and characteristics

LIANG Jun; WEI Shu-hua; YE Jian-ren; ZHANG Xing-yao

Volume 25 Issue 5

Oct. 2008

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(5): 559-564

LIANG Jun, WEI Shu-hua, YE Jian-ren, ZHANG Xing-yao. Crude toxin production of Botryosphaeria dothidea：initial conditions and characteristics[J]. Journal of Zhejiang A&F University, 2008, 25(5): 559-564.

Citation:

LIANG Jun, WEI Shu-hua, YE Jian-ren, ZHANG Xing-yao. Crude toxin production of Botryosphaeria dothidea：initial conditions and characteristics[J]. Journal of Zhejiang A&F University, 2008, 25(5): 559-564.

Crude toxin production of Botryosphaeria dothidea：initial conditions and characteristics

1.
Key Laboratory of Forest Ecology and Environment Protection，State Forestry Administration，Beijing 100091，China;
2.
Research Institute of Forest Ecology，Environment and Protection，The Chinese Academy of Forestry，Beijing 100091，China;
3.
Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences，Yinchuan 750002，Ningxia，China

Publish Date: 2008-10-20

Abstract

To study pathogenic mechanisms，initial conditions and characteristics of crude toxins produced from Botryosphaeria dothidea（Moug. ex Fr.） Ces. de Not. were first analyzed using the degree of browning on callus of Populus × beijingensis as the appraisal index. Results showed that with light，shaking，being cultured in a modified Fries No.3 liquid medium at 25 °C for 21 days，and a pH of 5.60 （acidic） and 7.00 （neutral），was the optimum condition for crude toxins production by B. dothidea. Additionally，produced more toxin. When treated with high temperature（121 °C） and high pressure（0.11 MPa） for 15 minutes，the toxins exhibited thermal instability and lost their bioactivity. However，with acidity and alkalinity they did remain active. Although toxicity decreased slightly，activity with this higher temperature and pressure was similar（P＞0.05） to toxins with pH of 4.69 and 8.69. ［Ch，5 fig. 3 tab. 18 ref.］
- forest protection,
- Populus × beijingensis,
- canker disease,
- toxin,
- Botryosphaeria spp,
- callus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Crude toxin production of Botryosphaeria dothidea：initial conditions and characteristics

1. Key Laboratory of Forest Ecology and Environment Protection，State Forestry Administration，Beijing 100091，China;

2. Research Institute of Forest Ecology，Environment and Protection，The Chinese Academy of Forestry，Beijing 100091，China;

3. Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences，Yinchuan 750002，Ningxia，China

Publish Date: 2008-10-20

Key words:

Abstract: To study pathogenic mechanisms，initial conditions and characteristics of crude toxins produced from Botryosphaeria dothidea（Moug. ex Fr.） Ces. de Not. were first analyzed using the degree of browning on callus of Populus × beijingensis as the appraisal index. Results showed that with light，shaking，being cultured in a modified Fries No.3 liquid medium at 25 °C for 21 days，and a pH of 5.60 （acidic） and 7.00 （neutral），was the optimum condition for crude toxins production by B. dothidea. Additionally，produced more toxin. When treated with high temperature（121 °C） and high pressure（0.11 MPa） for 15 minutes，the toxins exhibited thermal instability and lost their bioactivity. However，with acidity and alkalinity they did remain active. Although toxicity decreased slightly，activity with this higher temperature and pressure was similar（P＞0.05） to toxins with pH of 4.69 and 8.69. ［Ch，5 fig. 3 tab. 18 ref.］

Crude toxin production of Botryosphaeria dothidea：initial conditions and characteristics

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