Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis

HE Sha-e; ZHANG Zhi-jun

Volume 26 Issue 2

Apr. 2009

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HE Sha-e, ZHANG Zhi-jun. Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis[J]. Journal of Zhejiang A&F University, 2009, 26(2): 164-168.

Citation:

HE Sha-e, ZHANG Zhi-jun. Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis[J]. Journal of Zhejiang A&F University, 2009, 26(2): 164-168.

Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis

1.
The Key Laboratory for Modern Sivicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: ZHANG Zhi-jun
Publish Date: 2009-04-20

Abstract

An improved SDS-extraction method was used to obtain high quality DNA from five different bamboo soil samples. The crude DNA was purified by a simple method with DNA gel extraction kit，amplified with 16 S rDNA-based primers in V3 region by use of the polymerase chain reaction（PCR），and then the products of PCR was identified by denature gradient gel electrophoresis（DGGE）. The results showed that the DNA extracted from a little soil by this method was greater than 23 kb in size. The simple purification method we described here can remove greater part of contaminants from crude DNA and obtain high purity DNA. DGGE analysis patterns of amplified DNA presented abundant fragments. The extraction and purification method was considered to be an effective method for DNA isolation and purification from bamboo soil. It can be used to help characterize the biological composition and diversity of the microbial population in bamboo soil samples. ［Ch，3 fig. 1 tab. 15 ref.］
- microbiology,
- bamboo soil,
- microbial,
- DNA extraction,
- polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis

1. The Key Laboratory for Modern Sivicultural Technology of Zhejiang Province，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: ZHANG Zhi-jun

Publish Date: 2009-04-20

Key words:

microbiology /
bamboo soil /
microbial /
DNA extraction /
polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）

Abstract: An improved SDS-extraction method was used to obtain high quality DNA from five different bamboo soil samples. The crude DNA was purified by a simple method with DNA gel extraction kit，amplified with 16 S rDNA-based primers in V3 region by use of the polymerase chain reaction（PCR），and then the products of PCR was identified by denature gradient gel electrophoresis（DGGE）. The results showed that the DNA extracted from a little soil by this method was greater than 23 kb in size. The simple purification method we described here can remove greater part of contaminants from crude DNA and obtain high purity DNA. DGGE analysis patterns of amplified DNA presented abundant fragments. The extraction and purification method was considered to be an effective method for DNA isolation and purification from bamboo soil. It can be used to help characterize the biological composition and diversity of the microbial population in bamboo soil samples. ［Ch，3 fig. 1 tab. 15 ref.］

Extraction and purification of microbacterial total DNA from bamboo soil for PCR-DGGE analysis

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

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