Regeneration system for <EM>Dendrobium primulinum</EM>

ZHANG Ying; WANG Yan; LI Zhen-jian

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Volume 26 Issue 4

Aug. 2009

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Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(4): 603-606

ZHANG Ying, WANG Yan, LI Zhen-jian. Regeneration system for Dendrobium primulinum[J]. Journal of Zhejiang A&F University, 2009, 26(4): 603-606.

Citation:

ZHANG Ying, WANG Yan, LI Zhen-jian. Regeneration system for Dendrobium primulinum[J]. Journal of Zhejiang A&F University, 2009, 26(4): 603-606.

Regeneration system for Dendrobium primulinum

1.
Research Institute of Forestry，The Chinese Academy of Forestry，Beijing 100091，China;
2.
Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

More Information

Corresponding author: WANG Yan
Publish Date: 2009-08-20

Abstract

An in vitro plant regeneration system of Dendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog （MS） + 5.0 mg·L－1 6-BA + 1.5 mg·L－1 2，4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS + 1.0 mg·L－16-BA + 0.1 mg·L－1 NAA + coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS + 0.5 mg·L－1 6-BA +1.0 mg·L－1NAA + 0.5 g·L－1 activated carbon. ［Ch，1 fig. 4 tab. 12 ref.］
- botany,
- Dendrobium primulinum,
- stem sections,
- regeneration system

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Regeneration system for Dendrobium primulinum

1. Research Institute of Forestry，The Chinese Academy of Forestry，Beijing 100091，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: WANG Yan

Publish Date: 2009-08-20

Key words:

Abstract: An in vitro plant regeneration system of Dendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog （MS） + 5.0 mg·L－1 6-BA + 1.5 mg·L－1 2，4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS + 1.0 mg·L－16-BA + 0.1 mg·L－1 NAA + coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS + 0.5 mg·L－1 6-BA +1.0 mg·L－1NAA + 0.5 g·L－1 activated carbon. ［Ch，1 fig. 4 tab. 12 ref.］

ZHANG Ying, WANG Yan, LI Zhen-jian. Regeneration system for Dendrobium primulinum[J]. Journal of Zhejiang A&F University, 2009, 26(4): 603-606.

Citation:

ZHANG Ying, WANG Yan, LI Zhen-jian. Regeneration system for Dendrobium primulinum[J]. Journal of Zhejiang A&F University, 2009, 26(4): 603-606.

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Regeneration system for Dendrobium primulinum

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通讯作者: 陈斌, bchen63@163.com

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Regeneration system for Dendrobium primulinum

1. Research Institute of Forestry，The Chinese Academy of Forestry，Beijing 100091，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: WANG Yan

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Regeneration system for Dendrobium primulinum

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通讯作者: 陈斌, bchen63@163.com

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Regeneration system for Dendrobium primulinum

1. Research Institute of Forestry，The Chinese Academy of Forestry，Beijing 100091，China; 2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China

Corresponding author: WANG Yan

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1. Research Institute of Forestry，The Chinese Academy of Forestry，Beijing 100091，China;

2. Research Institute of Subtropical Forestry，The Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China