Simple sequence repeat analysis on pear cultivars in Niitaka line

MO Wen-juan; YUAN De-yi; DUAN Jing-hua; ZOU Feng

Volume 26 Issue 5

Oct. 2009

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MO Wen-juan, YUAN De-yi, DUAN Jing-hua, ZOU Feng. Simple sequence repeat analysis on pear cultivars in Niitaka line[J]. Journal of Zhejiang A&F University, 2009, 26(5): 639-643.

Citation:

MO Wen-juan, YUAN De-yi, DUAN Jing-hua, ZOU Feng. Simple sequence repeat analysis on pear cultivars in Niitaka line[J]. Journal of Zhejiang A&F University, 2009, 26(5): 639-643.

Simple sequence repeat analysis on pear cultivars in Niitaka line

1.
The Key Laboratory of Non-wood Forestry Product of State Forestry Administration，Central South Forestry & Technology University，Changsha 410004，Hunan，China;
2.
Non-timber Research and Development Center，The Chinese Academy of Forestry，Zhengzhou 450003，Henan，China

More Information

Corresponding author: YUAN De-yi
Publish Date: 2009-10-20

Abstract

In order to efficiently distinguish and use pear cultivars come of Pyrus pyrifolia ‘Niitaka’with a near genetic relationship，the polymorphism and cluster analysis based on genetic similarity matrices of simple sequence repeat（SSR） markers were used on nine cultivars. Results showed that 3 pairs of SSR primes（KA4b，NH013a and CH02D11） produced 22 amplified fragments of which 22（100%） were polymorphic. The average number of alleles per SSR locus was 7.3 with a range from 4 to 9. Using these 3 sets of SSR primers could discriminate 9 cultivars in the study. Also，the genetic similarity coefficients among the 9 cultivars ranged from 0.47 to 0.91 with an average of 0.67，which indicated that the genetic relationships were close between them. The UPGMA cluster analysis showed that the 9 cultivars could be divided into 3 groups and 2 sub-groups. The closest relationship was ‘Niitaka’，‘Whangkeum Bae’，‘Sujeong’，and ‘Huafeng’，whose genetic similarity coefficient is 0.91，while ‘Yewang’ and ‘Sunhwang’ were highly divergent from the other cultivars. ［Ch，3 fig. 3 tab. 17 ref.］
- horticulture,
- pear cultivars in Niitaka line,
- simple sequence repeat（SSR）,
- genetic relationship,
- cluster analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Simple sequence repeat analysis on pear cultivars in Niitaka line

1. The Key Laboratory of Non-wood Forestry Product of State Forestry Administration，Central South Forestry & Technology University，Changsha 410004，Hunan，China;

2. Non-timber Research and Development Center，The Chinese Academy of Forestry，Zhengzhou 450003，Henan，China

Corresponding author: YUAN De-yi

Publish Date: 2009-10-20

Key words:

Abstract: In order to efficiently distinguish and use pear cultivars come of Pyrus pyrifolia ‘Niitaka’with a near genetic relationship，the polymorphism and cluster analysis based on genetic similarity matrices of simple sequence repeat（SSR） markers were used on nine cultivars. Results showed that 3 pairs of SSR primes（KA4b，NH013a and CH02D11） produced 22 amplified fragments of which 22（100%） were polymorphic. The average number of alleles per SSR locus was 7.3 with a range from 4 to 9. Using these 3 sets of SSR primers could discriminate 9 cultivars in the study. Also，the genetic similarity coefficients among the 9 cultivars ranged from 0.47 to 0.91 with an average of 0.67，which indicated that the genetic relationships were close between them. The UPGMA cluster analysis showed that the 9 cultivars could be divided into 3 groups and 2 sub-groups. The closest relationship was ‘Niitaka’，‘Whangkeum Bae’，‘Sujeong’，and ‘Huafeng’，whose genetic similarity coefficient is 0.91，while ‘Yewang’ and ‘Sunhwang’ were highly divergent from the other cultivars. ［Ch，3 fig. 3 tab. 17 ref.］

Simple sequence repeat analysis on pear cultivars in Niitaka line

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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