Biocontrol agents introduced into a <i>Cucumis melo</i> rhizosphere

ZHANG Xin; ZHANG Li-qin; LIN Hai-ping; ZHANG Bing-xin

Dec. 2007

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(6): 649-653

ZHANG Xin, ZHANG Li-qin, LIN Hai-ping, ZHANG Bing-xin. Biocontrol agents introduced into a Cucumis melo rhizosphere[J]. Journal of Zhejiang A&F University, 2007, 24(6): 649-653.

Citation:

ZHANG Xin, ZHANG Li-qin, LIN Hai-ping, ZHANG Bing-xin. Biocontrol agents introduced into a Cucumis melo rhizosphere[J]. Journal of Zhejiang A&F University, 2007, 24(6): 649-653.

Biocontrol agents introduced into a Cucumis melo rhizosphere

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China;
2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China

Received Date: 2007-01-16
Rev Recd Date: 2007-04-29

Abstract

By quantifying cultural microorganisms and analyzing activities of enzymes in the soil, the ecological effect of biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, introduced into the Cucumis melo (cucumber) rhizosphere was studied to improve soil fertility. We used potculture method with three replications, one replication having ten pots of cucumber seedlings with the agent treated soil. 10 g soil was taken to measure every other week after the seedlings come out. Results showed that the introduced strains initially increased the bacterial population, but the influence gradually diminished with further plant growing. Introduction of the two strains caused no significant differences in fungi (P>0.01). Compared to the untreated soil, enzyme activities showed that during the plant growing season, the two biocontrol agents increased fructosidase, and dehydrogenase activity. Thus, the study indicated that introduction of the two strains could increase soil fertility.
- pedology,
- biocontrol agents,
- Cucumis melo (cucumber),
- rhizosphere,
- ecological effects

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References

[1]	DICK R P, TABATABAI M A. Significance and Potential Uses of Soil Enzymes:Application in Agriculture and Environmental Management[M]. New York:Marcel Dekker Press, 1993:95-125.
[2]	BOLTON H J, ELLIOTT L F, PAPENDICK R I, et al. Soil microbial biomass and selected soil enzyme activities:effect of fertilization and cropping practices[J]. Soil Biol Biochem, 1985,17:297-302.



[6]	NASEBY D C, MOENNE-LOCCOZ Y, POWELL J, et al. Soil enzyme activities in the rhizosphere of field-grown sugar beet inoculated with the biocontrol agent Pseudomonas fluorescens F113[J].Biol Fertil Soils, 1998,27:39-43.






[13]	ALEF K, NANNIPIERI P.Methods in Applied Soil Microbiology and Biochemistry[M]. London:Academic Press, 1995:214-219.
[14]	AMANN R I, LUDWIG W, SCHLEIFER K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiol Rrev,1995, 59(1):143-169.
[15]	MACURA J. Trends and advances in soil microbiology from 1924 to 1974[J].Geoderma,1974,12:311-329.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Biocontrol agents introduced into a Cucumis melo rhizosphere

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China;

2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China

Received Date: 2007-01-16

Rev Recd Date: 2007-04-29

Key words:

Abstract: By quantifying cultural microorganisms and analyzing activities of enzymes in the soil, the ecological effect of biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, introduced into the Cucumis melo (cucumber) rhizosphere was studied to improve soil fertility. We used potculture method with three replications, one replication having ten pots of cucumber seedlings with the agent treated soil. 10 g soil was taken to measure every other week after the seedlings come out. Results showed that the introduced strains initially increased the bacterial population, but the influence gradually diminished with further plant growing. Introduction of the two strains caused no significant differences in fungi (P>0.01). Compared to the untreated soil, enzyme activities showed that during the plant growing season, the two biocontrol agents increased fructosidase, and dehydrogenase activity. Thus, the study indicated that introduction of the two strains could increase soil fertility.

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Reference (15)

[1]	DICK R P, TABATABAI M A. Significance and Potential Uses of Soil Enzymes:Application in Agriculture and Environmental Management[M]. New York:Marcel Dekker Press, 1993:95-125.
[2]	BOLTON H J, ELLIOTT L F, PAPENDICK R I, et al. Soil microbial biomass and selected soil enzyme activities:effect of fertilization and cropping practices[J]. Soil Biol Biochem, 1985,17:297-302.
[3]	张志丹,赵兰坡.土壤酶在土壤有机培肥研究中的意义[J].土壤通报,2006,37(2):362-368.
[4]	杜国坚,黄天平,张庆荣,等.杉木混交林土壤微生物及生化特征和肥力[J].浙江林学院学报,1995,12(4):347-352.
[5]	姜培坤,俞益武,张立钦,等.雷竹林地土壤酶活性研究[J].浙江林学院学报, 2000,17(2):132-136.
[6]	NASEBY D C, MOENNE-LOCCOZ Y, POWELL J, et al. Soil enzyme activities in the rhizosphere of field-grown sugar beet inoculated with the biocontrol agent Pseudomonas fluorescens F113[J].Biol Fertil Soils, 1998,27:39-43.
[7]	薛冬,姚槐应,何振立,等.红壤酶活性与肥力的关系[J].应用生态学报, 2005,16(8):1455-1458.
[8]	王娟,谷雪景,赵吉.羊草草原土壤酶活性对土壤肥力的指示作用[J].农业环境科学学报, 2006,25(4):934-938.
[9]	张昕,张炳欣,喻景权,等.生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性[J].应用生态学报,2005,16(11):2144-2148.
[10]	张昕,张立钦,马良进,等.生防菌ZJY-1抑菌作用的初步研究[J].浙江林学院学报,2007,24(1):91-95.
[11]	张昕,张炳欣,赵宇华,等.多功能菌群混合施用的生态效应[J].应用生态学报,2005,16(10):1909-1912.
[12]	朱南文,闵航,陈美慈,等.甲胺磷对土壤中磷酸酶和脱氢酶活性的影响[J].农村生态环境,1996,12(2):22-29.
[13]	ALEF K, NANNIPIERI P.Methods in Applied Soil Microbiology and Biochemistry[M]. London:Academic Press, 1995:214-219.
[14]	AMANN R I, LUDWIG W, SCHLEIFER K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiol Rrev,1995, 59(1):143-169.
[15]	MACURA J. Trends and advances in soil microbiology from 1924 to 1974[J].Geoderma,1974,12:311-329.

Biocontrol agents introduced into a Cucumis melo rhizosphere

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Biocontrol agents introduced into a Cucumis melo rhizosphere

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China;

2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China

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Biocontrol agents introduced into a Cucumis melo rhizosphere

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通讯作者: 陈斌, bchen63@163.com

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Biocontrol agents introduced into a Cucumis melo rhizosphere

1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China; 2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China

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1. School of Forestry and Biotechnology, Zhejiang Forestry College, Lin'an 311300, Zhejiang, China;

2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China