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摘要: 用乙醇提取万寿菊Tagetes erecta的不同部位的粗提物,然后用浸泡生测的方法测定对松材线虫Bursaphelenchus xylophilus的生物活性。结果表明,万寿菊的根、茎、花的乙醇粗提物对松材线虫的活性较强,0.010 gL-1质量浓度以上处理72 h后发现,校正死亡率都能达到60%以上,其中万寿菊根的乙醇粗提物的活性最强,校正死亡率超过90%。万寿菊的各部分粗提物的得率都达到5%以上,这为利用植物提取物来毒杀松材线虫提供了初步的理论依据。图1表1参13Abstract: To determine the biological activity of Bursaphelenchus xylophilus with the live immersion method,three ethanol extraction of the roots,stems,and flowers of the marigold plant(Tagetes erecta) was used. A gradient test design with seven treatments of 1.000,0.500,0.100,0.050,0.010,0.001 and 0 gL-1 with four replications were established to immerse B. xylophilus (100 number in one culture dish,28 ℃). After 72 h,death rate of B. xylophilus was determined. Results showed that ethanol extracts immersed for 72 h had a corrected death rate for B. xylophilus of >60%. From among the three plant parts,ethanol extracts coming from the marigold roots had the greatest success with a corrected death rate for B. xylophilus>90%. Also,the ratio of the three extracts to the raw materials was>5%. Thus,we believe marigold plant extracts could be used to control B. xylophilus.[Ch,1 fig. 1 tab. 13 ref.]
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Key words:
- forest protection /
- Bursaphelenchus xylophilus /
- Tagetes erecta /
- extract /
- death rate
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2010.02.026
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