Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata
-
摘要: 纤维素合成酶类似蛋白（CSL）作为一种重要的膜蛋白与纤维素合成酶具有相类似的蛋白结构，都含有D，D，D，QXXRW保守区。利用其他物种中的CesA基因的保守序列设计简并引物，采用反转录-聚合酶链式反应（RT-PCR）结合cDNA末端快速扩增技术（RACE）成功地从杉木Cunninghamia lanceolata中扩增出一个含有完整阅读框架的cDNA序列，长度为4 150 bp，开放阅读框架为3 396 bp，编码1 132个氨基酸并含有保守的D，D，D，QXXRW天冬氨酸残基序列。应用美国国家生物技术信息中心（NCBI）数据库对获得的基因进行序列分析比对，发现它属于CslD基因家族，命名为ClCslD1基因。多重序列比对结果分析表明，该蛋白与来自颤杨Populus tremuloides，水稻Oryza sativa，拟南芥Arabidopsis thaliana等的同源基因相似性高达71%以上。利用生物软件对其进行生物学分析，为进一步研究其在植物纤化中的功能奠定基础。图5参10
-
关键词:
- 林木育种学 /
- 杉木 /
- 纤维素合成酶类似蛋白 /
- 克隆 /
- 表达
Abstract: The protein structure of the cellulose synthase-like protein （CSL） was similar to cellulose synthase （CesA），including the conservative sequence D，D，D，QXXRW. One full-length cDNA of the cellulose synthase-like protein D （CslD） gene was cloned by reverse transcriptase （RT）-polymerase chain reaction （PCR） with 5，3 rapid amplification of cDNA ends （RACE） methods using degenerate primers designed from the homologous sequences of the CesA genes. A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence. Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids. The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity （over 71%） with the ClCslD genes from Populus tremuloides，Oryza sativa，and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants. ［Ch，5 fig. 10 ref.］ -
-
链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2012.01.001
点击查看大图
计量
- 文章访问数: 4002
- HTML全文浏览量: 366
- PDF下载量: 1302
- 被引次数: 0
下载:
