Callus induction and plant regeneration in Sinopodophyllum hexandrum
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摘要: 以桃儿七Sinopodophyllum hexandrum成熟种胚为材料,研究了不同种类和浓度的细胞分裂素(噻苯隆TDZ和6-苄氨基腺嘌呤6?鄄BA)和生长素(萘乙酸NAA和吲哚乙酸IAA)对桃儿七愈合组织诱导和不定芽分化及无菌苗生根的影响,建立了完整组织培养再生体系。结果表明:在添加0.5 mgL-1TDZ+500 mgL-1水解酪蛋白(CH)的MS(Murashige和Skoog)培养基中,成熟种胚愈合诱导率最高,为97.8%,显著高于其他处理。在添加0.5 mgL-1TDZ的继代培养基中,出现球形胚和心形胚,但继代培养褐化严重,添加谷胱甘肽160 mgL-1能显著抑制褐化现象。在附加1.5 mgL-16-BA+0.2 mgL-1NAA+500 mgL-1CH的分化及增殖培养基中,不定芽诱导率最高,为53.3%,且增殖芽数最高为5.2。在添加0.5 mgL-1NAA,0.5mgL-1IAA等2种处理均可诱导生根,以MS+0.5 mgL-1IAA生根率最高,为22.4%。图1表3参13Abstract: To protect and exploit the endangered medicinal plant Sinopodophyllum hexandrum,a rapid regeneration system of tissue cultue was established. The effects of cytokinins [thidiazuron (TDZ) and 6-benzylaminopurine (6-BA)] and auxins [naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA)] on callus induction, adventitious bud induction, and rooting were studied. Using mature embryos of Sinopodophyllum hexandrum as explants Murashige and Skoog medium (MS) + 0.5 mgL-1 TDZ was used to induced embryogenic callus from primary callus so that embryogenic callus developed into globular embryoid and heart-stage embryoid. Results showed differences in percentages for mediums causing callus induction for S. hexandrum with TDZ concentrations of 0.5 mgL-1 TDZ and 500 mgL-1 casein hydrolysate (CH) having the best callus induction rate of 97.8%. To avoid brownness of callus a medium of 160 mgL-1 glutathione (GSH) was used. The optimum medium for adventitious bud differentiation and proliferation was MS + 1.5 mgL-1 6-BA + 0.2 mgL-1 NAA + 500 mgL-1 CH with the highest induction rate of 53.3% and 5.2 multiple shoots. Two treatments of 0.5mgL-1 NAA and 0.5 mgL-1 IAA induced roots, but the most suitable medium was MS + 0.5 mgL-1 IAA having a rooting rate of 22.4%. The rapid propagation system of Sinopodophyllum hexandrum has been preliminarily established, which provide theory foundation for biotechnology breeding and technical guidance for large-scale production [Ch, 1 fig. 3 tab. 13 ref.]
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Key words:
- botany /
- Sinopodophyllum hexandrum /
- tissue culture /
- callus /
- adventitious buds /
- browning
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链接本文:
https://zlxb.zafu.edu.cn/article/doi/10.11833/j.issn.2095-0756.2015.01.024
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