Effects of light intensity and nitrogen application on photosynthetic physiological parameters of Styrax obassia seedlings
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摘要:
目的 筛选更有利于玉铃花Styrax obassia光合生理参数表达的光强、施氮组合处理，揭示光强、氮素变化与玉铃花幼苗生长的关系。 方法 采用盆栽育苗方法，构建光氮双因素控制试验：4种遮光处理[全光对照(透光率100.00%)、轻度遮光(透光率为47.30%，L1)，中度遮光(20.00%，L2)和高度遮光(7.75%，L3)]与3种施氮处理[N2 (施纯氮0.63 g)，N1(施纯氮0.21 g)和N0(不施氮)]，测定分析玉铃花的光响应进程和光合色素质量分数的变化特征。 结果 ①随遮光程度和施氮量的增加，玉铃花幼苗的净光合速率、表观量子效率和总叶绿素质量分数呈现相应的增加，暗呼吸速率则相对减弱，其光饱和点为140.00~481.33 μmol·m−2·s−1，光补偿点为6.00~34.67 μmol·m−2·s−1、叶绿素a/b之比＜2。②全光及L1遮光+N2处理组合时，玉铃花植株出现枯死现象。③L1遮光处理下，净光合速率的从大到小变化顺序为N1、N0、N2；L3遮光下，当光合有效辐射＞400.00 μmol·m−2·s−1时，N2处理玉铃花的净光合速率显著高于N0处理和N1处理(P＜0.05)；④光强与氮肥施加对玉铃花幼苗光合色素质量分数的影响均达到极显著水平(P＜0.01)，影响趋势从大到小依次表现为光照、氮素、光照＋氮素。 结论 玉铃花幼苗具有较强的耐荫性，其光合生理参数最佳的光氮处理组合为高度遮光(L3)，并施纯氮0.63 g(N2)。图1表3参26 Abstract:Objective This study aims to screen the combination treatment of light intensity and nitrogen application that is more conducive to the expression of photosynthetic physiological parameters, so as to reveal the relationship between the changes of light intensity and nitrogen and the growth of Styrax obassia seedlings. Method Potted seedling cultivation method was adopted to construct the two-factor control test of light and nitrogen. Four shading treatments were prepared: all-light control with 100.00% transmittance, light shading with 47.30% transmittance (L1), moderate shading with 20.00% (L2) and high shading 7.75% (L3). Three nitrogen application treatments were included: N2 (0.63 g pure nitrogen), N1 (0.21 g pure nitrogen) and N0 (no nitrogen application). The light response process and the variation characteristics of photosynthetic pigment content were determined and analyzed. Result (1)With the increase of shading degree and nitrogen application rate, net photosynthetic rate (Pn), apparent quantum efficiency (AQY) and total chlorophyll (Chl) content of S. obassia seedlings increased, while dark respiration rate (Rd) decreased, and light saturation point (LSP) ranged from 140.00 to 481.33 μmol·m−2·s−1. Light compensation point (LCP) varied from 6.00 to 34.67 μmol·m−2·s−1, and the ratio of Chl a to Chl b was less than 2. (2) Under all light and L1 shading+N2 treatment, the plants died. (3) Under L1 shading, the change of Pn ranging from large to small was N1, N0, and N2. Under L3 shading, when photosynthetic effective radiation (PAR)was greater than 400 μmol·m−2·s−1, the Pn of N2 was significantly higher than that of N0 and N1 (P＜0.05). (4) Both light intensity and nitrogen application had significant effects on photosynthetic pigment content (P＜0.01), and the influence trend in descending order was light, nitrogen, and light+nitrogen. Conclusion The seedlings of S. obassia have strong shade tolerance, and the optimal combination treatment of light and nitrogen is L3+N2. [Ch, 1 fig. 3 tab. 26 ref.] -
图 1 光强与氮肥施加对玉铃花幼苗光响应的影响
Figure 1 Effects of light intensity and nitrogen application on light response of S. obassia seedlings
表 1 不同光强与氮肥施加下玉铃花幼苗光合光响应特征参数的变化
Table 1. Changes of photosynthetic light response characteristic parameters of S. obassia seedlings under different light intensity and nitrogen application
处理 LSP/(μmol·m−2·s−1) LCP/(μmol·m−2·s−1) AQY/(mmol−1) Pmax/(μmol·m−2·s−1) Rd/(μmol·m−2·s−1) 光强 氮素 L1 N0 288.00±30.87 b 34.67±5.44 a 0.043±0.301 a 1.649±0.155 de 0.464±0.001 b N1 220.00±28.28 b 29.33±5.76 ab 0.046±0.111 a 1.924±0.225 cde 0.512±0.005 ab N2 140.00±1.89 b 24.00±2.83 ab 0.066±0.080 a 1.247±0.068 e 0.352±0.014 ab L2 N0 164.00±8.49 b 16.00±2.83 ab 0.054±0.305 a 3.246±0.102 b 0.346±0.001 ab N1 226.66±38.84 b 16.00±9.98 ab 0.060±0.056 a 2.276±0.090 cd 0.297±0.009 ab N2 324.00±33.04 ab 14.67±2.18 ab 0.067±0.089 a 2.127±0.217 cde 0.295±0.019 ab L3 N0 270.00±29.70 b 12.00±8.49 ab 0.065±0.080 a 2.733±0.525 bc 0.276±0.002 b N1 306.66±47.19 ab 8.00±3.77 b 0.075±0.110 a 1.943±0.166 cde 0.170±0.011 ab N2 481.33±86.52 a 6.00±1.41 b 0.080±0.421 a 4.121±0.248 a 0.132±0.061 a 说明：数据为平均值±标准误，小写字母表示同一指标在不同处理之间差异显著(P＜0.05) 
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表 2 光强与氮肥施加对玉铃花幼苗光合色素质量分数的影响
Table 2. Effects of light and nitrogen interaction on photosynthetic pgment contents in seedlings of S. obassia
处理 CChl a/(mg·g−1) CChl b/(mg·g−1) (CChl a+CChl b)/(mg·g−1) CCar/(mg·g−1) CChl a/CChl b 光强 氮素 L1 N0 1.621±0.050 d 0.711±0.024 f 2.332±0.074 f 0.253±0.008 a 2.284±0.019 a N1 1.929±0.033 b 2.399±0.115 d 4.327±0.082 d 0.176±0.017 b 0.822±0.054 c N2 2.080±0.016 a 1.691±0.098 e 3.770±0.085 e 0.257±0.029 a 1.269±0.085 b L2 N0 1.799±0.031 c 2.960±0.126 c 4.760±0.098 c 0.083±0.023 cd 0.619±0.037 d N1 1.640±0.014 d 3.598±0.049 b 5.239±0.038 b 0.123±0.017 bc 0.457±0.010 e N2 1.577±0.020 de 3.806±0.082 b 5.382±0.064 b 0.075±0.018 cd 0.417±0.015 e L3 N0 1.525±0.011 e 4.114±0.024 a 5.638±0.018 c 0.073±0.012 cd 0.371±0.005 e N1 1.530±0.014 e 4.134±0.025 a 5.664±0.016 c 0.070±0.018 cd 0.370±0.005 e N2 1.496±0.020 e 4.136±0.056 a 5.632±0.040 c 0.058±0.024 a 0.362±0.009 e 说明：数据为平均值±标准误，小写字母表示同一指标在不同处理之间差异显著(P＜0.05)
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表 3 光强与氮肥施加对玉铃花幼苗光合色素质量分数影响的方差分析
Table 3. Anova analysis of the effects of light intensity and nitrogen application on photosynthetic pigment content of S. obassia seedlings
变异来源 因变量 平方和 自由度 均方 F P 光强 CChl a 0.722 2 0.361 102.841 0 CChl b 49.298 2 24.649 661.922 0 CCar 0.161 2 0.081 37.618 0 CChl a+CChl b 38.378 2 19.189 727.67 0 CChl a/CChl b 9.629 2 4.814 808.793 0 氮素 CChl a 0.080 2 0.040 11.322 0 CChl b 5.853 2 2.927 78.592 0 CCar 0.008 2 0.004 1.815 0.177 CChl a+CChl b 7.242 2 3.621 137.307 0 CChl a/CChl b 2.666 2 1.333 223.955 0 光强+氮素 CChl a 0.793 4 0.198 56.493 0 CChl b 4.565 4 1.141 30.645 0 CCar 0.032 4 0.008 3.775 0.012 CChl a+CChl b 7.301 4 1.825 69.216 0 CChl a/CChl b 3.859 4 0.965 162.081 0
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