Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions

XU Xing-tao; GUO Ming; WU Zhi-wu; LI Ming-hui; GAO Xing-jun; QIAN Ci

doi:10.11833/j.issn.2095-0756.2011.03.002

Volume 28 Issue 3

Jun. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(3): 349-358

XU Xing-tao, GUO Ming, WU Zhi-wu, LI Ming-hui, GAO Xing-jun, QIAN Ci. Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions[J]. Journal of Zhejiang A&F University, 2011, 28(3): 349-358. doi: 10.11833/j.issn.2095-0756.2011.03.002

Citation:

XU Xing-tao, GUO Ming, WU Zhi-wu, LI Ming-hui, GAO Xing-jun, QIAN Ci. Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions[J]. Journal of Zhejiang A&F University, 2011, 28(3): 349-358. doi: 10.11833/j.issn.2095-0756.2011.03.002

Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions

doi: 10.11833/j.issn.2095-0756.2011.03.002

1.
Department of Life Science，Tarim University，Alaer 843300，Xinjiang，China;
2.
School of Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: GUO Ming
Publish Date: 2011-06-20

Abstract

The interaction between rhaponticin （RT） and bovine serum albumin（BSA） was studied using fluorescence spectroscopy and absorbance spectra. Based on different theoretical models of fluorescence quenching，the binding constant （K） and binding sites （n） of the interactions were determined with the effects of metal ions on the interactions considered. The binding distance （r） and energy-transfer efficiency （E） between RT/RT-Co（Ⅱ）/RT-Ni（Ⅱ） and BSA were also obtained using the Fōrster theory of non-radiation energy transfer. Results showed that RT bound BSA by forming a stable complex and the micro-conformation of BSA changed greatly due to a hydrophobic change in the chemical environment of the fluorescence chromophores within the relevant subdomains of BSA. Therefore，metal ions Co（Ⅱ） and Ni（Ⅱ） could compete with RT-BSA interactions，thereby shortening the binding distance between RT and BSA；however，to precisely quantify the hydrophobic effect and micro-conformational changes of drugs on proteins，we suggest a new formula defining the entention degree ED.［Ch，8 fig. 3 tab. 31 ref.］
- rhaponticin,
- bovine serum albumin,
- fluorescence spectroscopy,
- ultraviolet absorption spectroscopy,
- metal ions

Relative Article

[1]	DENG Zaichun, ZHANG Chao, ZHU Xiali, FAN Jinming, QIAN Hui, LI Chengrong. Pinus yunnanensis volume estimation model based on UAV multispectral image . Journal of Zhejiang A&F University, 2024, 41(1): 49-56. doi: 10.11833/j.issn.2095-0756.20230263
[2]	QI Mengwen, SHEN Yi, YANG Guiying, YU Ting, LI Wuhan, ZHOU Qihuan, XIE Xiaojun, ZHU Yaning, MO Jianchu. Effects of metal ions on digestion and metabolism of Odontotermes formosanus . Journal of Zhejiang A&F University, 2024, 41(1): 154-160. doi: 10.11833/j.issn.2095-0756.20230219
[3]	WANG Jie, CHEN Keyi, JIN Hai, LI Zhaona, CHENG Minmin, YANG Haiyun. Reflection spectrum and photochemical characteristics of different colors’ leaves in Pseudosasa japonica . Journal of Zhejiang A&F University, 2021, 38(2): 271-279. doi: 10.11833/j.issn.2095-0756.20200360
[4]	FENG Yunyun, LIU Lijuan, LU Dengsheng, PANG Yong. A comparative study of dimension reduction methods for airborne hyperspectral images . Journal of Zhejiang A&F University, 2017, 34(5): 765-774. doi: 10.11833/j.issn.2095-0756.2017.05.001
[5]	NI Qianqian, QI Hengnian, ZHOU Zhu, WANG Hangjun. Identifying Dalbergia spp. wood with hyperspectral imaging technology . Journal of Zhejiang A&F University, 2016, 33(3): 489-494. doi: 10.11833/j.issn.2095-0756.2016.03.017
[6]	ZHENG Jian, ZHOU Zhu, ZHONG Shanmin, ZENG Songwei. Chestnut browning detected with near-infrared spectroscopy and a random-frog algorithm . Journal of Zhejiang A&F University, 2016, 33(2): 322-329. doi: 10.11833/j.issn.2095-0756.2016.02.019
[7]	ZHOU Jian, JIANG Zeping, WEI Yuan. Growth and ion transport with Pb stress in Robinia pseudoacacia seedlings . Journal of Zhejiang A&F University, 2016, 33(5): 742-748. doi: 10.11833/j.issn.2095-0756.2016.05.003
[8]	LUO Jinyan, YANG Chunlan, CHEN Lei, WANG Li, MAO Shengfeng, LI Bin. Detection and identification of mulberry bacterial wilt and its pathogen using Fourier transform infrared spectra . Journal of Zhejiang A&F University, 2015, 32(4): 578-584. doi: 10.11833/j.issn.2095-0756.2015.04.013
[9]	ZHOU Zhu, FANG Yiming, YIN Jianxin, ZHOU Suyin, LUO Ruisen, ZHENG Jian. Review of nondestructive detection of wood and wood products based on hyperspectral imaging technology . Journal of Zhejiang A&F University, 2015, 32(3): 458-466. doi: 10.11833/j.issn.2095-0756.2015.03.020
[10]	LI Yadan, DU Huaqiang, ZHOU Guomo, GU Chengyan, XU Xiaojun, SUN Shaobo, GAO Guolong. Chlorophyll content in Phyllostachys violascens related to hyper-spectral vegetation indices and development of an inversion model . Journal of Zhejiang A&F University, 2015, 32(3): 335-345. doi: 10.11833/j.issn.2095-0756.2015.03.002
[11]	YANG Jingyi, XIA Yufang, XIE Zhaojun, TAO Xingyue, DING Xiaoxia. Difference analysis using an FTIR spectrum for individual walnuts . Journal of Zhejiang A&F University, 2015, 32(3): 420-425. doi: 10.11833/j.issn.2095-0756.2015.03.014
[12]	WANG Yueting, ZHANG Xiaoli, YANG Huiqiao, WANG Shuhan, BAI Jinting. Forest volume estimation based on spectral and textural information from the Landsat 8 satellite . Journal of Zhejiang A&F University, 2015, 32(3): 384-391. doi: 10.11833/j.issn.2095-0756.2015.03.008
[13]	ZHOU Zhu, ZHENG Jian, WANG Yunxiang, ZENG Songwei. Phyllostachys violascens model optimization for bamboo shoot firmness using near-infrared spectroscopy . Journal of Zhejiang A&F University, 2015, 32(6): 875-882. doi: 10.11833/j.issn.2095-0756.2015.06.008
[14]	LI Huiyuan, ZHOU Dingguo, WU Qinglin. Properties of wood plastic composite with different photo-stabilizer and/or zinc borate . Journal of Zhejiang A&F University, 2015, 32(6): 914-918. doi: 10.11833/j.issn.2095-0756.2015.06.014
[15]	ZHANG Qianqian, CHEN Jian, JIANG Hong, TANG Minzhong. Classification of Hyperion hyperspectral imagery data using texture . Journal of Zhejiang A&F University, 2013, 30(6): 880-886. doi: 10.11833/j.issn.2095-0756.2013.06.012
[16]	GAO Xingjun, GUO Ming, LI Bing, GUO Jianzhong, LI Minghui. Interactions of bivalent metal ions and bovine serum albumins . Journal of Zhejiang A&F University, 2013, 30(5): 777-783. doi: 10.11833/j.issn.2095-0756.2013.05.022
[17]	GUO Ming, WU Xiao-peng, SUN Dong-hai, ZHOU Jian-zhong, ZHANG Hua. A new matrix solid-phase for heavy metal ion extraction and residual testing . Journal of Zhejiang A&F University, 2012, 29(4): 551-557. doi: 10.11833/j.issn.2095-0756.2012.04.011
[18]	DONG Da-chuan, KONG Zhen, YANG Wei-xi, LI Shuang-shuang, GAO Rong-fu, GAO Pei-jun. Spectral reflectance of leaves for Phyllostachys pubescens at different canopy layers using a 4th order derivative . Journal of Zhejiang A&F University, 2011, 28(6): 893-899. doi: 10.11833/j.issn.2095-0756.2011.06.009
[19]	DING Li－xia, WANG Zhi－hui, GE Hong－li. Continuum removal based hyperspectral characteristic analysis of leaves of different tree species . Journal of Zhejiang A&F University, 2010, 27(6): 809-814. doi: 10.11833/j.issn.2095-0756.2010.06.001
[20]	YUChong-yan, TONG Zai-kang, HUANG Hua-hong, ZHUYu-qiu. Quantification of phenolic compound in Magnolia officinalis herb by near infrared reflectance spectroscopy . Journal of Zhejiang A&F University, 2007, 24(5): 544-549.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(4371) PDF downloads(1645) Cited by()

Proportional views

Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions

1. Department of Life Science，Tarim University，Alaer 843300，Xinjiang，China;

2. School of Sciences，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: GUO Ming

Publish Date: 2011-06-20

Key words:

Abstract: The interaction between rhaponticin （RT） and bovine serum albumin（BSA） was studied using fluorescence spectroscopy and absorbance spectra. Based on different theoretical models of fluorescence quenching，the binding constant （K） and binding sites （n） of the interactions were determined with the effects of metal ions on the interactions considered. The binding distance （r） and energy-transfer efficiency （E） between RT/RT-Co（Ⅱ）/RT-Ni（Ⅱ） and BSA were also obtained using the Fōrster theory of non-radiation energy transfer. Results showed that RT bound BSA by forming a stable complex and the micro-conformation of BSA changed greatly due to a hydrophobic change in the chemical environment of the fluorescence chromophores within the relevant subdomains of BSA. Therefore，metal ions Co（Ⅱ） and Ni（Ⅱ） could compete with RT-BSA interactions，thereby shortening the binding distance between RT and BSA；however，to precisely quantify the hydrophobic effect and micro-conformational changes of drugs on proteins，we suggest a new formula defining the entention degree ED.［Ch，8 fig. 3 tab. 31 ref.］

Binding interactions between rhaponticin and bovine serum albumin with mediation of metal ions

doi: 10.11833/j.issn.2095-0756.2011.03.002

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views