A two-dimensional electrophoresis system of <em>Salix matsudana</em> root proteomics

ZHANG Xiao-guo; QIAO Gui-rong; LI Cui-yun; LIU Ming-ying; JIANG Jing; LI Hai-ying; QIU Wen-min; ZHUO Ren-ying

doi:10.11833/j.issn.2095-0756.2011.04.021

Volume 28 Issue 4

Aug. 2011

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Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(4): 653-661

ZHANG Xiao-guo, QIAO Gui-rong, LI Cui-yun, LIU Ming-ying, JIANG Jing, LI Hai-ying, QIU Wen-min, ZHUO Ren-ying. A two-dimensional electrophoresis system of Salix matsudana root proteomics[J]. Journal of Zhejiang A&F University, 2011, 28(4): 653-661. doi: 10.11833/j.issn.2095-0756.2011.04.021

Citation:

ZHANG Xiao-guo, QIAO Gui-rong, LI Cui-yun, LIU Ming-ying, JIANG Jing, LI Hai-ying, QIU Wen-min, ZHUO Ren-ying. A two-dimensional electrophoresis system of Salix matsudana root proteomics[J]. Journal of Zhejiang A&F University, 2011, 28(4): 653-661. doi: 10.11833/j.issn.2095-0756.2011.04.021

A two-dimensional electrophoresis system of Salix matsudana root proteomics

doi: 10.11833/j.issn.2095-0756.2011.04.021

1.
College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310052，Zhejiang，China;
2.
Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;
3.
College of Life Science and Technology，Xinjiang University，Urumqi 830046，Xinjiang，China

More Information

Corresponding author: ZHUO Ren-ying
Publish Date: 2011-08-20

Abstract

To establish a suitable two-dimensional electrophoresis technology, for bettering the use of proteomic technology in discovering new proteins of Salix matsudana roots induced by salt-stress and in revealing differences of protein expression of the roots with fresh water and salt-stress，this study adopted an improved phenol extraction method to extract total proteins from S. matsudana roots. Results were compared for different treatment conditions，and then two-dimensional electrophoresis maps of roots with treatments of 1/4 stregth Hoagland hydroponic medium and 1/4 stregth Hoagland hydroponic medium containing 100 mmolL-1 NaCl，were compared after 24 h of salt-stress. Results showed that the improved phenol extraction method could not only extract root proteins enough for 2-D，but it could also effectively remove salt from samples. Results showed that 900 g per immobilized pH gradient （IPG） gel，10 min equilibrium time，and 100 000 Vh isoelectric focusing（IEF） time were the best conditions combination for the protein isolation. With the twodimensional electrophoresis maps，39 proteins were differentially expressed, of which 15 were downregulated，and 24 were up-regulated with 11 newly induced.［Ch，9 fig. 23 ref.]
- forest tree breeding,
- Salix matsudana,
- proteomics,
- salt-stress

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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A two-dimensional electrophoresis system of Salix matsudana root proteomics

1. College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310052，Zhejiang，China;

2. Research Institute of Subtropical Forestry，Chinese Academy of Forestry，Fuyang 311400，Zhejiang，China;

3. College of Life Science and Technology，Xinjiang University，Urumqi 830046，Xinjiang，China

Corresponding author: ZHUO Ren-ying

Publish Date: 2011-08-20

Key words:

Abstract: To establish a suitable two-dimensional electrophoresis technology, for bettering the use of proteomic technology in discovering new proteins of Salix matsudana roots induced by salt-stress and in revealing differences of protein expression of the roots with fresh water and salt-stress，this study adopted an improved phenol extraction method to extract total proteins from S. matsudana roots. Results were compared for different treatment conditions，and then two-dimensional electrophoresis maps of roots with treatments of 1/4 stregth Hoagland hydroponic medium and 1/4 stregth Hoagland hydroponic medium containing 100 mmolL-1 NaCl，were compared after 24 h of salt-stress. Results showed that the improved phenol extraction method could not only extract root proteins enough for 2-D，but it could also effectively remove salt from samples. Results showed that 900 g per immobilized pH gradient （IPG） gel，10 min equilibrium time，and 100 000 Vh isoelectric focusing（IEF） time were the best conditions combination for the protein isolation. With the twodimensional electrophoresis maps，39 proteins were differentially expressed, of which 15 were downregulated，and 24 were up-regulated with 11 newly induced.［Ch，9 fig. 23 ref.]

A two-dimensional electrophoresis system of Salix matsudana root proteomics

doi: 10.11833/j.issn.2095-0756.2011.04.021

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doi: 10.11833/j.issn.2095-0756.2011.04.021

Corresponding author: ZHUO Ren-ying

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A two-dimensional electrophoresis system of Salix matsudana root proteomics

doi: 10.11833/j.issn.2095-0756.2011.04.021

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