Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，<em>ClCslD</em>1 from <em>Cunninghamia lanceolata</em>

PENG Sha-sha; TONG Zai-kang; HUANG Hua-hong; ZHOU Hou-jun; SHI Jian; LIN Er-pei

doi:10.11833/j.issn.2095-0756.2012.01.001

Volume 29 Issue 1

Feb. 2012

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PENG Sha-sha, TONG Zai-kang, HUANG Hua-hong, ZHOU Hou-jun, SHI Jian, LIN Er-pei. Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata[J]. Journal of Zhejiang A&F University, 2012, 29(1): 1-6. doi: 10.11833/j.issn.2095-0756.2012.01.001

Citation:

PENG Sha-sha, TONG Zai-kang, HUANG Hua-hong, ZHOU Hou-jun, SHI Jian, LIN Er-pei. Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata[J]. Journal of Zhejiang A&F University, 2012, 29(1): 1-6. doi: 10.11833/j.issn.2095-0756.2012.01.001

Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

doi: 10.11833/j.issn.2095-0756.2012.01.001

1.
School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: TONG Zai-kang
Received Date: 2011-02-10
Rev Recd Date: 2011-06-20
Publish Date: 2012-02-20

Abstract

The protein structure of the cellulose synthase-like protein （CSL） was similar to cellulose synthase （CesA），including the conservative sequence D，D，D，QXXRW. One full-length cDNA of the cellulose synthase-like protein D （CslD） gene was cloned by reverse transcriptase （RT）-polymerase chain reaction （PCR） with 5，3 rapid amplification of cDNA ends （RACE） methods using degenerate primers designed from the homologous sequences of the CesA genes. A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence. Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids. The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity （over 71%） with the ClCslD genes from Populus tremuloides，Oryza sativa，and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants. ［Ch，5 fig. 10 ref.］
- forest tree breeding,
- Cunninghamia lanceolata,
- cellulose synthase-like （CSL） protein,
- clone,
- expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: TONG Zai-kang

Received Date: 2011-02-10

Rev Recd Date: 2011-06-20

Publish Date: 2012-02-20

Key words:

Abstract: The protein structure of the cellulose synthase-like protein （CSL） was similar to cellulose synthase （CesA），including the conservative sequence D，D，D，QXXRW. One full-length cDNA of the cellulose synthase-like protein D （CslD） gene was cloned by reverse transcriptase （RT）-polymerase chain reaction （PCR） with 5，3 rapid amplification of cDNA ends （RACE） methods using degenerate primers designed from the homologous sequences of the CesA genes. A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence. Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids. The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity （over 71%） with the ClCslD genes from Populus tremuloides，Oryza sativa，and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants. ［Ch，5 fig. 10 ref.］

Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

doi: 10.11833/j.issn.2095-0756.2012.01.001

Abstract

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Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

doi: 10.11833/j.issn.2095-0756.2012.01.001

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: TONG Zai-kang

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Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

doi: 10.11833/j.issn.2095-0756.2012.01.001

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Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata

doi: 10.11833/j.issn.2095-0756.2012.01.001

1. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: TONG Zai-kang

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