Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species

WANG Wenpeng; ZHOU Lihua; ZHAO Hongbo; BAO Zhiyi

doi:10.11833/j.issn.2095-0756.2015.05.015

Volume 32 Issue 5

Sep. 2015

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WANG Wenpeng, ZHOU Lihua, ZHAO Hongbo, BAO Zhiyi. Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species[J]. Journal of Zhejiang A&F University, 2015, 32(5): 756-762. doi: 10.11833/j.issn.2095-0756.2015.05.015

Citation:

WANG Wenpeng, ZHOU Lihua, ZHAO Hongbo, BAO Zhiyi. Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species[J]. Journal of Zhejiang A&F University, 2015, 32(5): 756-762. doi: 10.11833/j.issn.2095-0756.2015.05.015

Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species

doi: 10.11833/j.issn.2095-0756.2015.05.015

1.
School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
The Nurturing Station for the Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2014-11-24
Rev Recd Date: 2015-03-09
Publish Date: 2015-10-20

Abstract

To breed new cultivars and increase breeding parent combinations, intergeneric cross-compatibility between Chimonanthus praecox and Calycanthus floridus var. glaucus, and between Chimonanthus praecox and Sinocalycanthus chinensis was analyzed by determining pollen-pistil interaction through fluorescence microscopy. To overcome the asynchronous flowering period, different methods，including low temperature and ultra-low temperature, were used to store pollen from these three species. Pollen viability in Chimonanthus praecox reached 12.7% after 3 months of crypreservation storage but was negligible for the other two species. When S. chinensis and Calycanthus floridus var. glaucus were used as female parents, pollen grains germinated and pollen tubes grew along the style. Pollen germination and growth characteristics between two intergeneric crosses were similar. About 6 h after pollination, pollen attached on stigmas and germinated 12 h after pollination; from 24 h to 36 h pollen tubes grew further along the styles reaching and entering the embryo sac at 48 h. Thus, Chimonanthus praecox pollen could be used as a male parent for crosses, and no barriers were found in the pollen-pistil interaction stage for crosses of Chimonanthus praecox and Calycanthus floridus var. glaucus or Chimonanthus praecox and S. chinensis. ［Ch, 5 fig. 1 tab. 24 ref.］
- horticulture,
- Chimonanthus praecox,
- Sinocalycanthus chinensis,
- Calycanthus floridus var. glaucus,
- intergeneric cross,
- pollen viability,
- cross-compatibility

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species

1. School of Landscape Architecture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. The Nurturing Station for the Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Received Date: 2014-11-24

Rev Recd Date: 2015-03-09

Publish Date: 2015-10-20

Key words:

Abstract: To breed new cultivars and increase breeding parent combinations, intergeneric cross-compatibility between Chimonanthus praecox and Calycanthus floridus var. glaucus, and between Chimonanthus praecox and Sinocalycanthus chinensis was analyzed by determining pollen-pistil interaction through fluorescence microscopy. To overcome the asynchronous flowering period, different methods，including low temperature and ultra-low temperature, were used to store pollen from these three species. Pollen viability in Chimonanthus praecox reached 12.7% after 3 months of crypreservation storage but was negligible for the other two species. When S. chinensis and Calycanthus floridus var. glaucus were used as female parents, pollen grains germinated and pollen tubes grew along the style. Pollen germination and growth characteristics between two intergeneric crosses were similar. About 6 h after pollination, pollen attached on stigmas and germinated 12 h after pollination; from 24 h to 36 h pollen tubes grew further along the styles reaching and entering the embryo sac at 48 h. Thus, Chimonanthus praecox pollen could be used as a male parent for crosses, and no barriers were found in the pollen-pistil interaction stage for crosses of Chimonanthus praecox and Calycanthus floridus var. glaucus or Chimonanthus praecox and S. chinensis. ［Ch, 5 fig. 1 tab. 24 ref.］

Intergeneric cross-compatibility from pollen-pistil interactions of three Calycanthaceae species

doi: 10.11833/j.issn.2095-0756.2015.05.015

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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