Preparation and performance of novel immobilized cellulase

FU Xiaoping; YAO Shanshan; WANG Ying; GUO Ming

doi:10.11833/j.issn.2095-0756.2016.02.018

Volume 33 Issue 2

Mar. 2016

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FU Xiaoping, YAO Shanshan, WANG Ying, GUO Ming. Preparation and performance of novel immobilized cellulase[J]. Journal of Zhejiang A&F University, 2016, 33(2): 315-321. doi: 10.11833/j.issn.2095-0756.2016.02.018

Citation:

FU Xiaoping, YAO Shanshan, WANG Ying, GUO Ming. Preparation and performance of novel immobilized cellulase[J]. Journal of Zhejiang A&F University, 2016, 33(2): 315-321. doi: 10.11833/j.issn.2095-0756.2016.02.018

Preparation and performance of novel immobilized cellulase

doi: 10.11833/j.issn.2095-0756.2016.02.018

1.
School of Sciences, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

浙江省大学生科技创新活动计划暨新苗人才计划资助项目（2014R412026, 201111030107）

Received Date: 2014-04-20
Rev Recd Date: 2015-06-29
Publish Date: 2016-04-20

Abstract

A novel carrier was synthesized by condensation reaction with an acid catalytic action using tetraethyl orthosilicate and KH-560 as the silica resource and coupling reagent, respectively. The composition and structure of the carrier were characterized by infrared absorption spectroscopy, 13C nuclear magnetic resonance, and X-ray diffractometry. The novel immobilized cellulase was prepared by the crosslinking-embedding immobilized method. The enzymatic characteristics of the immobilized cellulase were tested and compared with that of free cellulase. Experimental results showed that the immobilized cellulase maintained higher activity in the broader ranges of pH and temperature. The Michaelis constant （Km） of immobilized cellulase is similar as the was 1.58 gL－1 and free cellulase was 1.40 gL－1. Thus, immobilized cellulase could have a stronger affinity, reusability, and storage stability; and could provide a reference for preparing immobilized enzymes to perform better. ［Ch, 9 fig. 2 tab. 20 ref.］
- cellulase,
- crosslinking and embedding,
- immobilized,
- catalytic property

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Preparation and performance of novel immobilized cellulase

1. School of Sciences, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

浙江省大学生科技创新活动计划暨新苗人才计划资助项目（2014R412026, 201111030107）

Received Date: 2014-04-20

Rev Recd Date: 2015-06-29

Publish Date: 2016-04-20

Key words:

Abstract: A novel carrier was synthesized by condensation reaction with an acid catalytic action using tetraethyl orthosilicate and KH-560 as the silica resource and coupling reagent, respectively. The composition and structure of the carrier were characterized by infrared absorption spectroscopy, 13C nuclear magnetic resonance, and X-ray diffractometry. The novel immobilized cellulase was prepared by the crosslinking-embedding immobilized method. The enzymatic characteristics of the immobilized cellulase were tested and compared with that of free cellulase. Experimental results showed that the immobilized cellulase maintained higher activity in the broader ranges of pH and temperature. The Michaelis constant （Km） of immobilized cellulase is similar as the was 1.58 gL－1 and free cellulase was 1.40 gL－1. Thus, immobilized cellulase could have a stronger affinity, reusability, and storage stability; and could provide a reference for preparing immobilized enzymes to perform better. ［Ch, 9 fig. 2 tab. 20 ref.］

Preparation and performance of novel immobilized cellulase

doi: 10.11833/j.issn.2095-0756.2016.02.018

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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