Quantifying <em>Botryosphaeria dothidea</em> infection causing canker disease on <em>Carya cathayensis</em> using real-time PCR

ZHU Zhixiang; SHI Haojie; LEI Feibin; ZHANG Chuanqing

doi:10.11833/j.issn.2095-0756.2016.02.024

Volume 33 Issue 2

Mar. 2016

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ZHU Zhixiang, SHI Haojie, LEI Feibin, ZHANG Chuanqing. Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR[J]. Journal of Zhejiang A&F University, 2016, 33(2): 364-368. doi: 10.11833/j.issn.2095-0756.2016.02.024

Citation:

ZHU Zhixiang, SHI Haojie, LEI Feibin, ZHANG Chuanqing. Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR[J]. Journal of Zhejiang A&F University, 2016, 33(2): 364-368. doi: 10.11833/j.issn.2095-0756.2016.02.024

Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

doi: 10.11833/j.issn.2095-0756.2016.02.024

1.
School of Agricultural and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家林业局公益性行业科研专项（201304403）

Received Date: 2015-04-03
Rev Recd Date: 2015-05-31
Publish Date: 2016-04-20

Abstract

Canker disease caused by Botryosphaeria dothidea is the most important disease that threaten the production of Chinese hickory and has the significant characteristics of latent infection. Therefore, the development of a quantitative detection technique of B. dothidea in hickory plants is the prerequisite for its forecasting and scientific management. In this study, specific primers were respectively designed based on the conserved regions of EF1 gene of B. dothiaea. Results of both real-time PCR and common PCR showed that the specificity and sensitivity of EFRT-F1/R1 was good primer pair. A band of 230 bp could be stably amplified by the primers EFRT-F1/R1. Further study displayed that this real-time PCR technique is more sensitive （more than 100 fold） than the common one. Our molecular detection technique will provide scientific basis for forecast, and management of hickory canker disease. ［Ch, 6 fig. 8 ref.］
- forest protection,
- comker disease,
- real-time PCR,
- specific primers,
- genome DNA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

1. School of Agricultural and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Funds:

国家林业局公益性行业科研专项（201304403）

Received Date: 2015-04-03

Rev Recd Date: 2015-05-31

Publish Date: 2016-04-20

Key words:

Abstract: Canker disease caused by Botryosphaeria dothidea is the most important disease that threaten the production of Chinese hickory and has the significant characteristics of latent infection. Therefore, the development of a quantitative detection technique of B. dothidea in hickory plants is the prerequisite for its forecasting and scientific management. In this study, specific primers were respectively designed based on the conserved regions of EF1 gene of B. dothiaea. Results of both real-time PCR and common PCR showed that the specificity and sensitivity of EFRT-F1/R1 was good primer pair. A band of 230 bp could be stably amplified by the primers EFRT-F1/R1. Further study displayed that this real-time PCR technique is more sensitive （more than 100 fold） than the common one. Our molecular detection technique will provide scientific basis for forecast, and management of hickory canker disease. ［Ch, 6 fig. 8 ref.］

Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

doi: 10.11833/j.issn.2095-0756.2016.02.024

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Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

doi: 10.11833/j.issn.2095-0756.2016.02.024

1. School of Agricultural and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

doi: 10.11833/j.issn.2095-0756.2016.02.024

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Quantifying Botryosphaeria dothidea infection causing canker disease on Carya cathayensis using real-time PCR

doi: 10.11833/j.issn.2095-0756.2016.02.024

1. School of Agricultural and Food Science, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

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