Pooling designs based on clone library screenings

GUAN Yu

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Volume 24 Issue 1

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2007 > 24(1): 105-109

GUAN Yu. Pooling designs based on clone library screenings[J]. Journal of Zhejiang A&F University, 2007, 24(1): 105-109.

Citation:

GUAN Yu. Pooling designs based on clone library screenings[J]. Journal of Zhejiang A&F University, 2007, 24(1): 105-109.

Pooling designs based on clone library screenings

GUAN Yu^,

1.
School of Science, Zhejiang Forestry College, Linan 311300, Zhejiang, China

Funds:

浙江省自然科学基金资助项目(Y104420);浙江省教育厅资助项目(20040507)

Received Date: 2006-02-18
Rev Recd Date: 2006-05-22
Publish Date: 2007-02-20

Abstract

A pooling design based on clone library screenings is an experimental strategy to find cloneswith special nucleotide strings; it is also an algorithm of combinatorial group testing.A binary conjunctional matrix, sometimes called a disjunctmatrix, with a row corresponding to an experiment and a column to a clone, is usually used to represent the pooling design. Since errors exist during biological experiments, a disjunct matrix should have detecting and correcting capabilities.Utilizing the inclusion relationship between two subsets or subspaces, disjunct matrices with the same numberof elements 1 in each rowand columnwere constructed.However, the experimental number of elementswas greater than the lower bound of information.[Ch, 13 ref.]
- clone library,
- pooling design,
- group testing,
- disjunctmatrix,
- bound of information

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Pooling designs based on clone library screenings

GUAN Yu^,

1. School of Science, Zhejiang Forestry College, Linan 311300, Zhejiang, China

Funds:

浙江省自然科学基金资助项目(Y104420);浙江省教育厅资助项目(20040507)

Received Date: 2006-02-18
Rev Recd Date: 2006-05-22
Publish Date: 2007-02-20

Key words:

Abstract: A pooling design based on clone library screenings is an experimental strategy to find cloneswith special nucleotide strings; it is also an algorithm of combinatorial group testing.A binary conjunctional matrix, sometimes called a disjunctmatrix, with a row corresponding to an experiment and a column to a clone, is usually used to represent the pooling design. Since errors exist during biological experiments, a disjunct matrix should have detecting and correcting capabilities.Utilizing the inclusion relationship between two subsets or subspaces, disjunct matrices with the same numberof elements 1 in each rowand columnwere constructed.However, the experimental number of elementswas greater than the lower bound of information.[Ch, 13 ref.]

GUAN Yu. Pooling designs based on clone library screenings[J]. Journal of Zhejiang A&F University, 2007, 24(1): 105-109.

Citation:

GUAN Yu. Pooling designs based on clone library screenings[J]. Journal of Zhejiang A&F University, 2007, 24(1): 105-109.

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