Micropropagation of <em>Dendrobium officinale</em>

WANG Chun; ZHENG Yong-ping; LUOMan; ZHOUFang-yong; YUQiong-fang

Volume 24 Issue 3

Feb. 2017

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WANG Chun, ZHENG Yong-ping, LUOMan, ZHOUFang-yong, YUQiong-fang. Micropropagation of Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2007, 24(3): 372-376.

Citation:

WANG Chun, ZHENG Yong-ping, LUOMan, ZHOUFang-yong, YUQiong-fang. Micropropagation of Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2007, 24(3): 372-376.

Micropropagation of Dendrobium officinale

1.
Zhejiang Senhe Seed Co., Ltd, Hangzhou 310020, Zhejiang, China

Funds:

浙江省科学技术重点项目(2005C22082)

Received Date: 2006-06-07
Rev Recd Date: 2007-01-16
Publish Date: 2007-06-20

Abstract

Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch, 1 fig. 5 tab. 11ref.]
- botany,
- Dendrobium officinale,
- tissue culture,
- adventitious buds,
- protocom

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Micropropagation of Dendrobium officinale

1. Zhejiang Senhe Seed Co., Ltd, Hangzhou 310020, Zhejiang, China

Funds:

浙江省科学技术重点项目(2005C22082)

Received Date: 2006-06-07

Rev Recd Date: 2007-01-16

Publish Date: 2007-06-20

Key words:

Abstract: Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch, 1 fig. 5 tab. 11ref.]

Micropropagation of Dendrobium officinale

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