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Article Navigation >  Journal of Zhejiang A&F University  > 2007  >  24(3): 372-376

PAN Binrong, REN Jingyu, ZHAO Guangwu. Germination and vigor of sweet corn seeds with seed soaking time[J]. Journal of Zhejiang A&F University, 2015, 32(1): 47-51. DOI: 10.11833/j.issn.2095-0756.2015.01.007
Citation: WANG Chun, ZHENG Yong-ping, LUOMan, et al. Micropropagation of Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2007, 24(3): 372-376.
shu

Micropropagation of Dendrobium officinale

  • 1.

    Zhejiang Senhe Seed Co., Ltd, Hangzhou 310020, Zhejiang, China

Funds:

浙江省科学技术重点项目(2005C22082)

  • Received Date: 2006-06-07
  • Rev Recd Date: 2007-01-16
  • Publish Date: 2007-06-20
  • Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch, 1 fig. 5 tab. 11ref.]
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Micropropagation of Dendrobium officinale

  • 1. Zhejiang Senhe Seed Co., Ltd, Hangzhou 310020, Zhejiang, China
Funds:

浙江省科学技术重点项目(2005C22082)

  • Received Date: 2006-06-07
  • Rev Recd Date: 2007-01-16
  • Publish Date: 2007-06-20

Abstract: Dendrobium officinale(iron-sheet dendrobium) is a rare plant species for medical use. Its present resource cannot satisfy the needs. Tissue culture is a quick and convenient path to produce more iron-sheetdendrobium. The tiny seeds were sowed on the basic culture medias of MS(Murashige and Skoog), half of MS(1 2 MS), Hyponexl and Hyponex 2. And the fresh stems were sterilized in differentways, then cultured in the basic media added NAA or BA in different ammounts. The resluts showed that 1 2 MS+ A(the additive substance)+ 25 gL- 1 sugar was the best medium for seeding; Method B was the best disinfection with the livability 76.2%; 1/2 MS+ 1.5 mgL- 1 BA+ 0.25 mgL- 1 NAA was better for adventitious buds induction,with 4.7 buds on each explant; 1 2 MS+ 1.0 mgL- 1BA+ 0.5 mgL- 1NAA was fine for protocorm induction,with the rate of 8%; 1/2 MS+ 0.5 mgL- 1 NAAwas betterfor rooting, with a 100% rate and 7.1 roots on each explants. The proliferation systerm can enlarge the application of Dendrobium officinale.[Ch, 1 fig. 5 tab. 11ref.]

PAN Binrong, REN Jingyu, ZHAO Guangwu. Germination and vigor of sweet corn seeds with seed soaking time[J]. Journal of Zhejiang A&F University, 2015, 32(1): 47-51. DOI: 10.11833/j.issn.2095-0756.2015.01.007
Citation: WANG Chun, ZHENG Yong-ping, LUOMan, et al. Micropropagation of Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2007, 24(3): 372-376.
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