Tissue culture and rapid propagation of <em>Atriplex canescens</em>

PENG Shao-bing; WANG De-xiang

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Volume 24 Issue 3

Feb. 2017

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PENG Shao-bing, WANG De-xiang. Tissue culture and rapid propagation of Atriplex canescens[J]. Journal of Zhejiang A&F University, 2007, 24(3): 377-381.

Citation:

PENG Shao-bing, WANG De-xiang. Tissue culture and rapid propagation of Atriplex canescens[J]. Journal of Zhejiang A&F University, 2007, 24(3): 377-381.

Tissue culture and rapid propagation of Atriplex canescens

PENG Shao-bing,
WANG De-xiang^,

1.
College of Forestry, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi, China

Funds:

农业部引进国际先进农业科学技术计划(948计划)项目(2003-Z99)

Received Date: 2006-08-28
Rev Recd Date: 2007-01-02
Publish Date: 2007-06-20

Abstract

Atriplex canescens(fourwing saltbush)is a salt, base and drought tolerant plant species, so it can be planted widely in the northwestof China. Use half and fully lignified stems as experimental materials, to study the influencing of the time of the explantwas taken, the method of the optimal sterilization of explant, and the NAA and 6-BA concentrations on the tissue culture of fourwing saltbush. The results showed that the bestpicking time is in April, the optimal sterilization method of explant is dipped in 70% alcohol about 30 seconds and then in 1.0 gL- 1 HgCl2about 6 minutes. The culture media MS(Murashige and Skoog)containing 0.5 mgL- 1 6-BA are suitable for the initial culture(P 0.01), and MS medium supplemented with 1.0 mgL- 1 6-BA and 0.10 mgL- 1 NAAin subculture was in favorof the shotproliferation and growth(P 0.01). The material has high rooting percentages(36.7%) and excellentdevelopment rootsystemson 1/2MSmediumwith 0.10 mgL- 1 NAA.[Ch,4 fig. 4 tab. 10 ref.]
- botany,
- Atriplexcanescens,
- tissue culture,
- plant growth regulators

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Tissue culture and rapid propagation of Atriplex canescens

PENG Shao-bing,
WANG De-xiang^,

1. College of Forestry, Northwest Agricultural and Forestry University, Yangling 712100, Shaanxi, China

Funds:

农业部引进国际先进农业科学技术计划(948计划)项目(2003-Z99)

Received Date: 2006-08-28
Rev Recd Date: 2007-01-02
Publish Date: 2007-06-20

Key words:

Abstract: Atriplex canescens(fourwing saltbush)is a salt, base and drought tolerant plant species, so it can be planted widely in the northwestof China. Use half and fully lignified stems as experimental materials, to study the influencing of the time of the explantwas taken, the method of the optimal sterilization of explant, and the NAA and 6-BA concentrations on the tissue culture of fourwing saltbush. The results showed that the bestpicking time is in April, the optimal sterilization method of explant is dipped in 70% alcohol about 30 seconds and then in 1.0 gL- 1 HgCl2about 6 minutes. The culture media MS(Murashige and Skoog)containing 0.5 mgL- 1 6-BA are suitable for the initial culture(P 0.01), and MS medium supplemented with 1.0 mgL- 1 6-BA and 0.10 mgL- 1 NAAin subculture was in favorof the shotproliferation and growth(P 0.01). The material has high rooting percentages(36.7%) and excellentdevelopment rootsystemson 1/2MSmediumwith 0.10 mgL- 1 NAA.[Ch,4 fig. 4 tab. 10 ref.]

PENG Shao-bing, WANG De-xiang. Tissue culture and rapid propagation of Atriplex canescens[J]. Journal of Zhejiang A&F University, 2007, 24(3): 377-381.

Citation:

PENG Shao-bing, WANG De-xiang. Tissue culture and rapid propagation of Atriplex canescens[J]. Journal of Zhejiang A&F University, 2007, 24(3): 377-381.

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