Establishment of plant regeneration system in matured <em>Liriodendron</em> hybrids

LI Ji-yuan; TIAN Min; LI Xin-lei; FAN Zheng-qi; NI Sui

Volume 23 Issue 5

Feb. 2017

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LI Ji-yuan, TIAN Min, LI Xin-lei, FAN Zheng-qi, NI Sui. Establishment of plant regeneration system in matured Liriodendron hybrids[J]. Journal of Zhejiang A&F University, 2006, 23(5): 512-515.

Citation:

LI Ji-yuan, TIAN Min, LI Xin-lei, FAN Zheng-qi, NI Sui. Establishment of plant regeneration system in matured Liriodendron hybrids[J]. Journal of Zhejiang A&F University, 2006, 23(5): 512-515.

Establishment of plant regeneration system in matured Liriodendron hybrids

1.
Research Institute of Subtropical Forestry , The Chinese Academy of Forestry , Fuyang 311400 , Zhejiang ,China;
2.
.Department of Biological Science and Engineering , Ningbo University , Ningbo 315211, Zhejiang ,China

Received Date: 2006-02-16
Rev Recd Date: 2006-05-21
Publish Date: 2006-10-20

Abstract

The in vitro plant regeneration system of Liriodendron hybrids planted in 1967 at the yard of Research Institute of Subtropical Forestry was established by using the leaves and petioles as explants .The results showed that callus could be induced from these explants on the media supplemented with different concentrations of plant growth regulators .The adventitious buds could be generated from the calli induced on the media MS +0.5 mgL-1 NAA +2.0 -4.0 mg L-1 6-BA .On the medium MS +0.5 mgL-1 NAA +4.0 mgL-1 6-BA , the adventitious buds could be directly differentiated from the explants without inducing calli .The best medium for rooting was 1/2MS +1.5 mgL-1 NAA .[ Ch , 2 fig .2 tab .13 ref .]
- forest tree breeding,
- Liriodendron,
- hybrids,
- differentiation,
- plant regeneration

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Establishment of plant regeneration system in matured Liriodendron hybrids

1. Research Institute of Subtropical Forestry , The Chinese Academy of Forestry , Fuyang 311400 , Zhejiang ,China;

2. .Department of Biological Science and Engineering , Ningbo University , Ningbo 315211, Zhejiang ,China

Received Date: 2006-02-16

Rev Recd Date: 2006-05-21

Publish Date: 2006-10-20

Key words:

Abstract: The in vitro plant regeneration system of Liriodendron hybrids planted in 1967 at the yard of Research Institute of Subtropical Forestry was established by using the leaves and petioles as explants .The results showed that callus could be induced from these explants on the media supplemented with different concentrations of plant growth regulators .The adventitious buds could be generated from the calli induced on the media MS +0.5 mgL-1 NAA +2.0 -4.0 mg L-1 6-BA .On the medium MS +0.5 mgL-1 NAA +4.0 mgL-1 6-BA , the adventitious buds could be directly differentiated from the explants without inducing calli .The best medium for rooting was 1/2MS +1.5 mgL-1 NAA .[ Ch , 2 fig .2 tab .13 ref .]

Establishment of plant regeneration system in matured Liriodendron hybrids

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Establishment of plant regeneration system in matured Liriodendron hybrids

1. Research Institute of Subtropical Forestry , The Chinese Academy of Forestry , Fuyang 311400 , Zhejiang ,China;

2. .Department of Biological Science and Engineering , Ningbo University , Ningbo 315211, Zhejiang ,China

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Establishment of plant regeneration system in matured Liriodendron hybrids

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Establishment of plant regeneration system in matured Liriodendron hybrids

1. Research Institute of Subtropical Forestry , The Chinese Academy of Forestry , Fuyang 311400 , Zhejiang ,China; 2. .Department of Biological Science and Engineering , Ningbo University , Ningbo 315211, Zhejiang ,China

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1. Research Institute of Subtropical Forestry , The Chinese Academy of Forestry , Fuyang 311400 , Zhejiang ,China;

2. .Department of Biological Science and Engineering , Ningbo University , Ningbo 315211, Zhejiang ,China