Quick detection of<em> Bursaphelenchus xylophilus</em> by the method of dissecting pine twigs

LAI Yan-xue

Volume 22 Issue 2

Feb. 2017

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LAI Yan-xue. Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs[J]. Journal of Zhejiang A&F University, 2005, 22(2): 188-192.

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LAI Yan-xue. Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs[J]. Journal of Zhejiang A&F University, 2005, 22(2): 188-192.

Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

LAI Yan-xue^,

1.
Forest Pest Control and Quarantine Station of Ningbo City , Ningbo 315000 , Zhejiang , China

Received Date: 2004-06-11
Rev Recd Date: 2004-09-30
Publish Date: 2005-06-20

Abstract

Early diagnosis of pine wood nematode (PWN)is a key measure to prevent and control pine wood nematode .To detect PWN in the pine trees early and quickly , the technology of twig dissection with the aid of portable microscope was invented , which could diagnose pine wood nematode quickly .The method was as follows : cutting pine twigs with pruning shears , slicing the twigs up with scalpel , then examining them with the portable microscope .The results showed that the detection rate of nematode was 70 % in the infected twigs , 100 % in the dying twigs , 60 %in partial dead twigs and naught in healthy twigs .The average time for detecting a sample in pine forests was 2.5 minutes .Therefore , the technology of twig dissection with the aid of portable microscope could diagnose pine wood nematode quickly .[ Ch , 3 tab .25 ref .]
- forest protection,
- pine wood nematode (Bursaphelechus xylopilus),
- dissection,
- portable microscope,
- early diagnosis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

1. Forest Pest Control and Quarantine Station of Ningbo City , Ningbo 315000 , Zhejiang , China

Received Date: 2004-06-11

Rev Recd Date: 2004-09-30

Publish Date: 2005-06-20

Key words:

forest protection /
pine wood nematode (Bursaphelechus xylopilus) /
dissection /
portable microscope /
early diagnosis

Abstract: Early diagnosis of pine wood nematode (PWN)is a key measure to prevent and control pine wood nematode .To detect PWN in the pine trees early and quickly , the technology of twig dissection with the aid of portable microscope was invented , which could diagnose pine wood nematode quickly .The method was as follows : cutting pine twigs with pruning shears , slicing the twigs up with scalpel , then examining them with the portable microscope .The results showed that the detection rate of nematode was 70 % in the infected twigs , 100 % in the dying twigs , 60 %in partial dead twigs and naught in healthy twigs .The average time for detecting a sample in pine forests was 2.5 minutes .Therefore , the technology of twig dissection with the aid of portable microscope could diagnose pine wood nematode quickly .[ Ch , 3 tab .25 ref .]

Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

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Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

1. Forest Pest Control and Quarantine Station of Ningbo City , Ningbo 315000 , Zhejiang , China

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Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

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Quick detection of Bursaphelenchus xylophilus by the method of dissecting pine twigs

1. Forest Pest Control and Quarantine Station of Ningbo City , Ningbo 315000 , Zhejiang , China

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