High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of <em>Torreya grandis</em> `Merrillii'

HUANG Hua-hong; TONG Zai-kang; LIAO Wang-yi; BI Chun-hui; LOU Xiong-zhen

Volume 22 Issue 4

Feb. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2005 > 22(4): 390-395

HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.

Citation:

HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

1.
School of Forestry and Biotechnology , Zhejiang Forestry College , Lin,an 311300, Zhejiang , China;
2.
.Zhejiang Senhe Seed Co ., Ltd, Hangzhou 310020, Zhejiang , China;
3.
Water China Co ., Ltd, Shanghai 200001, China

Funds:

浙江省科学技术厅重大项目(021102537)

Received Date: 2004-09-30
Rev Recd Date: 2005-05-19
Publish Date: 2005-08-20

Abstract

Extraction , purification and chromatographic conditions of endogenous IAA , ABA and GA3 in the buds were studied , with female floral buds of Torreya grandis `Merrillii' during the differentiation as materials .The results showed that endogenous hormones could be better purified after treating with petroleum ether , water-insoluble PVP and Sep-PakC18 column .The chromatographic conditions were as follows :ODS-C18 reversed-phase column , Water 486 ultraviolet detector , the mobile phase was a mixture of methanol , water and acetic acid (methanol∶water=50∶50 , volume ratio , pH 3.0), velocity of flow was set at l mLmin-1 , temperature of column and injection volume was 25 ℃ and 20 L respectively , detecting wavelength of IAA and ABA was set at 280 nm, while that of GA3 was set at 210nm .The determining results showed that the method could be apply to trace analysis .In addition , it was found that from February 21 to April 29 the contents of IAA and GA3 in the female floral buds increased then decreased , while that of ABA increased , slightly decreased then increased obviously .[ Ch , 4 fig . 4 tab .11 ref .]
- plant physiology,
- Torreya grandis `Merrillii ',
- female floral buds,
- high performance liquid chromatographic analysis,
- endogenous hormones

Relative Article

[1]	WANG Xiaorong, QIU Hong, ZHANG Qixiang, WU Jiasheng, LOU Heqiang. Physiological differences in rooting and response to drought in shoots of female and male Torreya grandis‘Merrillii’ . Journal of Zhejiang A&F University, 2024, 41(3): 478-485. doi: 10.11833/j.issn.2095-0756.20230471
[2]	WANG Wenyue, FENG Zhongping, WANG Jianchang, YANG Tao, ZHANG Zhen, ZHOU Zhichun. Effects of top pruning and exogenous hormone application on endogenous hormone content and female bulb formation in Pinus massoniana . Journal of Zhejiang A&F University, 2023, 40(6): 1188-1196. doi: 10.11833/j.issn.2095-0756.20220768
[3]	QIAN Yuting, XUE Xiaofeng, ZENG Yanru, CHEN Wenchong, YE Xiaoming, YU Weiwu, DAI Wensheng. Leaf structure and chlorophyll content in Torreya grandis 'Merrillii' with Nalepella abiesis infestation . Journal of Zhejiang A&F University, 2020, 37(2): 296-302. doi: 10.11833/j.issn.2095-0756.2020.02.014
[4]	GU Hongxia, YE Wen, QIAN Yuting, YE Xiaoming, DAI Wensheng. Micrografting methods with Torreya grandis ‘Merrillii’ . Journal of Zhejiang A&F University, 2018, 35(1): 183-188. doi: 10.11833/j.issn.2095-0756.2018.01.025
[5]	LI Zuyao, ZHANG Yanhua, ZHANG Lei, LI Yuan, SUN Yadong. Endogenous hormones in young bamboo development of Phyllostachys edulis 'Pachyloen' . Journal of Zhejiang A&F University, 2018, 35(6): 1107-1114. doi: 10.11833/j.issn.2095-0756.2018.06.014
[6]	GONG Li, HU Hengkang, HU Yuanyuan, YU Weiwu, WU Jiasheng, HUANG Jianqin, ZHANG Qixiang. Immature embryo development and embryogenic frequency in Torreya grandis 'Merrillii' . Journal of Zhejiang A&F University, 2018, 35(5): 861-867. doi: 10.11833/j.issn.2095-0756.2018.05.010
[7]	JIN Houding, YU Weiwu, ZENG Yanru, XIANG Meiyun, DAI Wensheng, DANG Wanyu. Cutting-based propagation in Torreya grandis 'Merrillii' . Journal of Zhejiang A&F University, 2017, 34(1): 185-191. doi: 10.11833/j.issn.2095-0756.2017.01.025
[8]	ZENG Songwei, YU Weiwu, JI Changying, YE Bangxuan, XIAO Qinglai. A peeling machine for Torreya grandis ‘Merrillii’ nuts . Journal of Zhejiang A&F University, 2015, 32(1): 133-139. doi: 10.11833/j.issn.2095-0756.2015.01.020
[9]	WU Lianhai, WU Liming, NI Rongxin, YAN Fuhua. Economic benefits of Torreya grandis ‘Merrillii’ plantings . Journal of Zhejiang A&F University, 2013, 30(2): 299-303. doi: 10.11833/j.issn.2095-0756.2013.02.023
[10]	LU Ya-ting, YUAN Xiao-liang, LIN Xin-chun, FANG Wei. Endogenous hormone changes during floral bud morphological differentiation of Phyllostachys violascens . Journal of Zhejiang A&F University, 2012, 29(2): 161-165. doi: 10.11833/j.issn.2095-0756.2012.02.002
[11]	ZHANG Ai-lian, QIAN Hui-qin, ZHU Yan, WU Ling-shang, SI Jin-ping, ZHAO Qiang. Variation of quercetin content in Houttuynia cordata from twenty-two provenances . Journal of Zhejiang A&F University, 2009, 26(3): 314-318.
[12]	WANG Xiao-ming, WANG Ke, QIN Sui-chu, JIANG Yu-gen. Review of research on favorable environmental factors of Torreya grandis ‘Merrillii’ . Journal of Zhejiang A&F University, 2008, 25(3): 382-386.
[13]	HUANG Yan, JI Kong-shu, FANG Yan, ZHAI Jin-ru. Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting . Journal of Zhejiang A&F University, 2007, 24(3): 284-289.
[14]	DAI Wen-sheng, LI Zhang-ju, CHENG Xiao-jian, YU Wei-wu, FU Qing-gong. Mineral elements in Torreya grandis 'Merrillii' seeds and their forest soils . Journal of Zhejiang A&F University, 2006, 23(4): 393-399.
[15]	DAI Wen-sheng, LI Zhang-ju, CHENG Xiao-jian, YU Wei-wu, FU Qing-gong, CHEN Qin-juan. Development future and strategies of production of Torreya grandis 'Merrillii' in Hangzhou . Journal of Zhejiang A&F University, 2006, 23(3): 334-337.
[16]	LUO Ping-yuan, SHI Ji-kong, ZHANG Wan-ping. Changes of endogenous hormones , carbohydrate and mineral nutrition during the differentiation of female flower buds of Ginkgo biloba . Journal of Zhejiang A&F University, 2006, 23(5): 532-537.
[17]	LI Jian-gui, HUANG Jun-hua, WANG Qiang, RUAN Xiao. Seasonal changes of endogenetic hormones in leaves of Haloxylon ammodendron in Xinjiang . Journal of Zhejiang A&F University, 2005, 22(3): 282-285.
[18]	LI Zhang-ju, CHENGXiao-jian, DAI Wen-sheng, JING Bao-hua, WANG An-guo. History and status and development of Torreya grandis in Zhejiang Province . Journal of Zhejiang A&F University, 2004, 21(4): 471-474.
[19]	MENG Hong-fei, JIN Guo-long, WENG Zhong-yuan. Investigation on resource of ancient Torreya grandis trees in Zhuji City , China . Journal of Zhejiang A&F University, 2003, 20(2): 134-136.
[20]	Ni Deliang, Xu Jianping, Ouyang Zhong, Zou Yulin, Ren Qinliang. Development and Application of WiId Young Trees of Torreya grandis . Journal of Zhejiang A&F University, 1994, 11(2): 206-210.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1557) PDF downloads(252) Cited by()

Proportional views

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin,an 311300, Zhejiang , China;

2. .Zhejiang Senhe Seed Co ., Ltd, Hangzhou 310020, Zhejiang , China;

3. Water China Co ., Ltd, Shanghai 200001, China

Funds:

浙江省科学技术厅重大项目(021102537)

Received Date: 2004-09-30

Rev Recd Date: 2005-05-19

Publish Date: 2005-08-20

Key words:

plant physiology /
Torreya grandis `Merrillii ' /
female floral buds /
high performance liquid chromatographic analysis /
endogenous hormones

Abstract: Extraction , purification and chromatographic conditions of endogenous IAA , ABA and GA3 in the buds were studied , with female floral buds of Torreya grandis `Merrillii' during the differentiation as materials .The results showed that endogenous hormones could be better purified after treating with petroleum ether , water-insoluble PVP and Sep-PakC18 column .The chromatographic conditions were as follows :ODS-C18 reversed-phase column , Water 486 ultraviolet detector , the mobile phase was a mixture of methanol , water and acetic acid (methanol∶water=50∶50 , volume ratio , pH 3.0), velocity of flow was set at l mLmin-1 , temperature of column and injection volume was 25 ℃ and 20 L respectively , detecting wavelength of IAA and ABA was set at 280 nm, while that of GA3 was set at 210nm .The determining results showed that the method could be apply to trace analysis .In addition , it was found that from February 21 to April 29 the contents of IAA and GA3 in the female floral buds increased then decreased , while that of ABA increased , slightly decreased then increased obviously .[ Ch , 4 fig . 4 tab .11 ref .]

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin,an 311300, Zhejiang , China;

2. .Zhejiang Senhe Seed Co ., Ltd, Hangzhou 310020, Zhejiang , China;

3. Water China Co ., Ltd, Shanghai 200001, China

HTML

Catalog

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin,an 311300, Zhejiang , China; 2. .Zhejiang Senhe Seed Co ., Ltd, Hangzhou 310020, Zhejiang , China; 3. Water China Co ., Ltd, Shanghai 200001, China

HTML

Catalog

Export File

Citation

Format

Content

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin,an 311300, Zhejiang , China;

2. .Zhejiang Senhe Seed Co ., Ltd, Hangzhou 310020, Zhejiang , China;

3. Water China Co ., Ltd, Shanghai 200001, China