Volume 22 Issue 4
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HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.
Citation: HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.

High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

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浙江省科学技术厅重大项目(021102537)

  • Received Date: 2004-09-30
  • Rev Recd Date: 2005-05-19
  • Publish Date: 2005-08-20
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'

Funds:

浙江省科学技术厅重大项目(021102537)

Abstract: Extraction , purification and chromatographic conditions of endogenous IAA , ABA and GA3 in the buds were studied , with female floral buds of Torreya grandis `Merrillii' during the differentiation as materials .The results showed that endogenous hormones could be better purified after treating with petroleum ether , water-insoluble PVP and Sep-PakC18 column .The chromatographic conditions were as follows :ODS-C18 reversed-phase column , Water 486 ultraviolet detector , the mobile phase was a mixture of methanol , water and acetic acid (methanol∶water=50∶50 , volume ratio , pH 3.0), velocity of flow was set at l mLmin-1 , temperature of column and injection volume was 25 ℃ and 20 L respectively , detecting wavelength of IAA and ABA was set at 280 nm, while that of GA3 was set at 210nm .The determining results showed that the method could be apply to trace analysis .In addition , it was found that from February 21 to April 29 the contents of IAA and GA3 in the female floral buds increased then decreased , while that of ABA increased , slightly decreased then increased obviously .[ Ch , 4 fig . 4 tab .11 ref .]

HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.
Citation: HUANG Hua-hong, TONG Zai-kang, LIAO Wang-yi, BI Chun-hui, LOU Xiong-zhen. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis `Merrillii'[J]. Journal of Zhejiang A&F University, 2005, 22(4): 390-395.

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