Tissue culture and propagation in grape

WU Yue-yan; TAO Wei-fang

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Volume 18 Issue 2

Jul. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2001 > 18(2): 188-192

WU Yue-yan, TAO Wei-fang. Tissue culture and propagation in grape[J]. Journal of Zhejiang A&F University, 2001, 18(2): 188-192.

Citation:

WU Yue-yan, TAO Wei-fang. Tissue culture and propagation in grape[J]. Journal of Zhejiang A&F University, 2001, 18(2): 188-192.

Tissue culture and propagation in grape

1.
Department of Life Science , Zhejiang Wanli University , Ningbo 315101, Zhejiang , China

Funds:

浙江省宁波市重点实验室基金资助项目(9941004)

Received Date: 2000-08-28
Rev Recd Date: 2001-02-05
Publish Date: 2001-06-20

Abstract

Taking the stems of 0.5 cm with single bud as the explants to the different culturemediums , the results showed that the rates of differentiation of buds that were the highest in all treatments were 100 % for cv . Wuhebaijixin , and cv .Meirenzhi and 86 % for cv .Yongyou F1 on the GS medium containing 6-BA 1.0 mgL-1 or 6-BA 2.0 mgL-1 .When the buds grew 1.0 ～ 1.5cm , they were transferred to the MS medium supplemented with IAA 0.05 mgL-1 or 6-BA 1.0mgL-1 orGA 2.0 mgL-1 with the differentiation rate of 80 % ～ 86 % and the proliferation multiple of 4 for this three varieties and the shoots grew vigor , which were the most favourite medium for the induction and differention of buds in this test .If the grape of Wuhebaijixin was changed into the 1/2 MS medium containing IBA 1.0 mgL-1 and Meirenzhi and Yongyou F1 were changed into 1/2 MS medium containing IBA 2.0 mgL-1 , the shoots developed nice roots with rooting rate of 84 %～ 96 % and rooting number of 5 ～ 7 .These media were the most significant for roting in all treatments .
- grape,
- tissue cultures,
- cuture in vitro,
- culture media,
- differentiation (biology),
- rooting

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Tissue culture and propagation in grape

1. Department of Life Science , Zhejiang Wanli University , Ningbo 315101, Zhejiang , China

Funds:

浙江省宁波市重点实验室基金资助项目(9941004)

Received Date: 2000-08-28
Rev Recd Date: 2001-02-05
Publish Date: 2001-06-20

Key words:

Abstract: Taking the stems of 0.5 cm with single bud as the explants to the different culturemediums , the results showed that the rates of differentiation of buds that were the highest in all treatments were 100 % for cv . Wuhebaijixin , and cv .Meirenzhi and 86 % for cv .Yongyou F1 on the GS medium containing 6-BA 1.0 mgL-1 or 6-BA 2.0 mgL-1 .When the buds grew 1.0 ～ 1.5cm , they were transferred to the MS medium supplemented with IAA 0.05 mgL-1 or 6-BA 1.0mgL-1 orGA 2.0 mgL-1 with the differentiation rate of 80 % ～ 86 % and the proliferation multiple of 4 for this three varieties and the shoots grew vigor , which were the most favourite medium for the induction and differention of buds in this test .If the grape of Wuhebaijixin was changed into the 1/2 MS medium containing IBA 1.0 mgL-1 and Meirenzhi and Yongyou F1 were changed into 1/2 MS medium containing IBA 2.0 mgL-1 , the shoots developed nice roots with rooting rate of 84 %～ 96 % and rooting number of 5 ～ 7 .These media were the most significant for roting in all treatments .

WU Yue-yan, TAO Wei-fang. Tissue culture and propagation in grape[J]. Journal of Zhejiang A&F University, 2001, 18(2): 188-192.

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WU Yue-yan, TAO Wei-fang. Tissue culture and propagation in grape[J]. Journal of Zhejiang A&F University, 2001, 18(2): 188-192.

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1. Department of Life Science , Zhejiang Wanli University , Ningbo 315101, Zhejiang , China

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