Protocorm-like body （PLB） development on <EM>Dendrobium officinale</EM>

ZHANG Qi-xiang; FU Su-jing; FANG Yan-ming; CHEN Na

Volume 26 Issue 3

Jun. 2009

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Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(3): 444-448

ZHANG Qi-xiang, FU Su-jing, FANG Yan-ming, CHEN Na. Protocorm-like body （PLB） development on Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2009, 26(3): 444-448.

Citation:

ZHANG Qi-xiang, FU Su-jing, FANG Yan-ming, CHEN Na. Protocorm-like body （PLB） development on Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2009, 26(3): 444-448.

Protocorm-like body （PLB） development on Dendrobium officinale

1.
School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;
2.
Department of Biology and Chemistry，Tongren University，Tongren 554300，Guizhou，China;
3.
College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

More Information

Corresponding author: FANG Yan-ming
Publish Date: 2009-06-20

Abstract

To determine the most effective medium for protocorm-like body（PLB） induction and growth，shoot tips of Dendrobium officinale were cultured on media. Then，several compound addenda were added to the media to determine the best treatment for PLB multiplication，and this was followed by anatomical observation of PLB formation on D. officinale. Results showed that the best medium for PLB induction and embling regeneration was 1/2MS + 0.5 mg·L－1 6-BA + 0.05 mg·L－1 NAA + 0.1 mg·L－1 KT + 1.0 g·L－1 LH with potato juice. The anatomical observation showed that the PLBs developed from the tissue behind the apical meristem，which could dedifferentiate into embryogenetic cells. The process was as follows：creamy global structures were visible after 40 d，and these gradually increased. Cells on the edge of the PLBs continued to dedifferentiate forming a global colony. These global structures grew into bumps when the PLBs multiplied. ［Ch，1 fig. 2 tab. 13 ref.］
- botany,
- Dendrobium officinale,
- protocorm-like body（PLB）,
- development,
- tissue culture

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Protocorm-like body （PLB） development on Dendrobium officinale

1. School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. Department of Biology and Chemistry，Tongren University，Tongren 554300，Guizhou，China;

3. College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: FANG Yan-ming

Publish Date: 2009-06-20

Key words:

Abstract: To determine the most effective medium for protocorm-like body（PLB） induction and growth，shoot tips of Dendrobium officinale were cultured on media. Then，several compound addenda were added to the media to determine the best treatment for PLB multiplication，and this was followed by anatomical observation of PLB formation on D. officinale. Results showed that the best medium for PLB induction and embling regeneration was 1/2MS + 0.5 mg·L－1 6-BA + 0.05 mg·L－1 NAA + 0.1 mg·L－1 KT + 1.0 g·L－1 LH with potato juice. The anatomical observation showed that the PLBs developed from the tissue behind the apical meristem，which could dedifferentiate into embryogenetic cells. The process was as follows：creamy global structures were visible after 40 d，and these gradually increased. Cells on the edge of the PLBs continued to dedifferentiate forming a global colony. These global structures grew into bumps when the PLBs multiplied. ［Ch，1 fig. 2 tab. 13 ref.］

Protocorm-like body （PLB） development on Dendrobium officinale

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