Protocorm-like body （PLB） development on <EM>Dendrobium officinale</EM>

ZHANG Qi-xiang; FU Su-jing; FANG Yan-ming; CHEN Na

Volume 26 Issue 3

Jun. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2009 > 26(3): 444-448

ZHANG Qi-xiang, FU Su-jing, FANG Yan-ming, CHEN Na. Protocorm-like body （PLB） development on Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2009, 26(3): 444-448.

Citation:

ZHANG Qi-xiang, FU Su-jing, FANG Yan-ming, CHEN Na. Protocorm-like body （PLB） development on Dendrobium officinale[J]. Journal of Zhejiang A&F University, 2009, 26(3): 444-448.

Protocorm-like body （PLB） development on Dendrobium officinale

1.
School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;
2.
Department of Biology and Chemistry，Tongren University，Tongren 554300，Guizhou，China;
3.
College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

More Information

Corresponding author: FANG Yan-ming
Publish Date: 2009-06-20

Abstract

To determine the most effective medium for protocorm-like body（PLB） induction and growth，shoot tips of Dendrobium officinale were cultured on media. Then，several compound addenda were added to the media to determine the best treatment for PLB multiplication，and this was followed by anatomical observation of PLB formation on D. officinale. Results showed that the best medium for PLB induction and embling regeneration was 1/2MS + 0.5 mg·L－1 6-BA + 0.05 mg·L－1 NAA + 0.1 mg·L－1 KT + 1.0 g·L－1 LH with potato juice. The anatomical observation showed that the PLBs developed from the tissue behind the apical meristem，which could dedifferentiate into embryogenetic cells. The process was as follows：creamy global structures were visible after 40 d，and these gradually increased. Cells on the edge of the PLBs continued to dedifferentiate forming a global colony. These global structures grew into bumps when the PLBs multiplied. ［Ch，1 fig. 2 tab. 13 ref.］
- botany,
- Dendrobium officinale,
- protocorm-like body（PLB）,
- development,
- tissue culture

Relative Article

[1]	FENG Rui, ZHOU Qi, WU Lingshang, ZHU Yuqiu, GAO Yanhui. Effects of PEG 6000 simulated drought stress on physiological and chlorophyll fluorescence characteristics of Dendrobium candidum seedlings . Journal of Zhejiang A&F University, 2024, 41(1): 132-144. doi: 10.11833/j.issn.2095-0756.20230301
[2]	ZENG Kang, WANG Shuwen, ZHOU Bingying, WANG Huasen, XU Yunmin. Research progress on tendril formation in Cucurbitaceae horticultural crops . Journal of Zhejiang A&F University, 2020, 37(6): 1216-1223. doi: 10.11833/j.issn.2095-0756.20190756
[3]	XIN Xiaoxue, WANG Xuexiang, LI Mingyu, GUO Hongyan, WANG Xiaojie, SU Tai, XU Ping, CHENG Jin. Active components of flowers in different flowering stages and floral structures of Dendrobium officinalei . Journal of Zhejiang A&F University, 2019, 36(1): 200-205. doi: 10.11833/j.issn.2095-0756.2019.01.025
[4]	WANG Bei, HE Yu, WU Rong, SHEN Yafang, WANG Yang, ZHAO Guangwu. Research progress on biological characteristics, occurrence and control of Oryza sativa f. spontanea . Journal of Zhejiang A&F University, 2019, 36(5): 1028-1036. doi: 10.11833/j.issn.2095-0756.2019.05.024
[5]	ZHANG Lu, DING Han, GUI Herong. Callus induction from leaf and plant regeneration of Sedum plumbizincicola . Journal of Zhejiang A&F University, 2018, 35(3): 567-571. doi: 10.11833/j.issn.2095-0756.2018.03.024
[6]	GAO Yang, LIN Ren'an, YUAN Chaoqun, MA Liya, WANG Jia'nan, ZHU Yuqiu, GAO Yanhui. Cytological study on self and hybridization affinity in Dendrobium officinale . Journal of Zhejiang A&F University, 2018, 35(4): 618-623. doi: 10.11833/j.issn.2095-0756.2018.04.006
[7]	JING Ning, KANG Jin, ZHANG Yaohong, KANG Yongxiang. Callus induction and plant regeneration in Sinopodophyllum hexandrum . Journal of Zhejiang A&F University, 2015, 32(1): 162-166. doi: 10.11833/j.issn.2095-0756.2015.01.024
[8]	RUAN Huize, LI Zhen, REN Yanyan, WU Xiaonan, SHAO Yuhao, PAN Feixiang, XIA Guohua. Plantlet regeneration of Hemiboea subcapitata with subculturing . Journal of Zhejiang A&F University, 2014, 31(1): 162-166. doi: 10.11833/j.issn.2095-0756.2014.01.025
[9]	CHEN Jialong, ZHU Jianjun, WU Xiushui, YU Hong′ao. Tissue culture and rapid propagation of Caragana sinica . Journal of Zhejiang A&F University, 2013, 30(4): 611-614. doi: 10.11833/j.issn.2095-0756.2013.04.022
[10]	ZHANG Lingju, GAO Tingting, ZHANG Xiaoling, SI Jinping. Photosynthetic characteristics of Dendrobium officinale from five provenances . Journal of Zhejiang A&F University, 2013, 30(3): 359-363. doi: 10.11833/j.issn.2095-0756.2013.03.009
[11]	LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium . Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022
[12]	XU Yong, SUN Jun-wei, CHEN Zhen. Plant growth regulator treatments for a tissue culture of Euodia rutaecarpa . Journal of Zhejiang A&F University, 2011, 28(3): 500-504. doi: 10.11833/j.issn.2095-0756.2011.03.024
[13]	CHENG Ying, LI Gen-you, XIA Guo-hua, HUANG Shang-jue, HUANG Yu-feng. Review on tissue culture of Aralia plants . Journal of Zhejiang A&F University, 2011, 28(6): 968-972. doi: 10.11833/j.issn.2095-0756.2011.06.022
[14]	ZHANG Nan, DU Bao-ming, JI Meng-cheng. Research progress on the bryophytes tissue culture . Journal of Zhejiang A&F University, 2011, 28(2): 305-313. doi: 10.11833/j.issn.2095-0756.2011.02.022
[15]	YANG Hai-yun, GUI Ren-yi, TANG Ding-qin, FANG Wei. Micropropagation of Sasa fortunei . Journal of Zhejiang A&F University, 2008, 25(2): 255-258.
[16]	WANG Chun, ZHENG Yong-ping, LUOMan, ZHOUFang-yong, YUQiong-fang. Micropropagation of Dendrobium officinale . Journal of Zhejiang A&F University, 2007, 24(3): 372-376.
[17]	GAO Yan-hui, TONG Zai-kang, HUANG Hua-hong, GUO Fu-qi, YU Chong-yan. Tissue culture on Trachelospermum jasminoides `Variegatum' . Journal of Zhejiang A&F University, 2006, 23(6): 701-704.
[18]	LIU Zhi-gao, CHU Jia-miao, GAO Yan-hui, ZHANG Lu, . Tissue culture in vitro of Lycoris albiflora . Journal of Zhejiang A&F University, 2006, 23(3): 347-350.
[19]	Zhang Liqin, Zheng Yong ping, Luo Shiyuan, Hu Jiagong.. Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures . Journal of Zhejiang A&F University, 1997, 14(1): 16-21.
[20]	Zhang Liqin, Zheng YongPing, Jin Peiying. selection of Salt Tolerance poplar Regenerated plant-lets in Tissues Culture. . Journal of Zhejiang A&F University, 1996, 13(4): 397-404.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(5283) PDF downloads(171) Cited by()

Proportional views

Protocorm-like body （PLB） development on Dendrobium officinale

1. School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China;

2. Department of Biology and Chemistry，Tongren University，Tongren 554300，Guizhou，China;

3. College of Forest Resources and Environment，Nanjing Forestry University，Nanjing 210037，Jiangsu，China

Corresponding author: FANG Yan-ming

Publish Date: 2009-06-20

Key words:

Abstract: To determine the most effective medium for protocorm-like body（PLB） induction and growth，shoot tips of Dendrobium officinale were cultured on media. Then，several compound addenda were added to the media to determine the best treatment for PLB multiplication，and this was followed by anatomical observation of PLB formation on D. officinale. Results showed that the best medium for PLB induction and embling regeneration was 1/2MS + 0.5 mg·L－1 6-BA + 0.05 mg·L－1 NAA + 0.1 mg·L－1 KT + 1.0 g·L－1 LH with potato juice. The anatomical observation showed that the PLBs developed from the tissue behind the apical meristem，which could dedifferentiate into embryogenetic cells. The process was as follows：creamy global structures were visible after 40 d，and these gradually increased. Cells on the edge of the PLBs continued to dedifferentiate forming a global colony. These global structures grew into bumps when the PLBs multiplied. ［Ch，1 fig. 2 tab. 13 ref.］

Protocorm-like body （PLB） development on Dendrobium officinale

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Protocorm-like body （PLB） development on Dendrobium officinale

Corresponding author: FANG Yan-ming

HTML

Catalog

Protocorm-like body （PLB） development on Dendrobium officinale

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Protocorm-like body （PLB） development on Dendrobium officinale

Corresponding author: FANG Yan-ming

HTML

Catalog

Export File

Citation

Format

Content