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木本植物不定根发生机制研究进展

苗大鹏 贾瑞瑞 李胜皓 席烁 朱葛 文书生

苗大鹏, 贾瑞瑞, 李胜皓, 席烁, 朱葛, 文书生. 木本植物不定根发生机制研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210652
引用本文: 苗大鹏, 贾瑞瑞, 李胜皓, 席烁, 朱葛, 文书生. 木本植物不定根发生机制研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210652
MIAO Dapeng, JIA Ruirui, LI Shenghao, XI Shuo, ZHU Ge, WEN Shusheng. Research advances in the mechanism of adventitious root occurrence in woody species[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210652
Citation: MIAO Dapeng, JIA Ruirui, LI Shenghao, XI Shuo, ZHU Ge, WEN Shusheng. Research advances in the mechanism of adventitious root occurrence in woody species[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210652

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木本植物不定根发生机制研究进展

doi: 10.11833/j.issn.2095-0756.20210652
基金项目: 国家自然科学基金资助项目(32001359);江苏省自然科学基金资助项目(BK20180771);江苏高校优势学科建设工程资助项目(PAPD);南京林业大学青年科技创新基金(CX2018026);南京林业大学大学生创新训练计划项目(2021NFUSPITP0015)
详细信息
    作者简介: 苗大鹏(ORCID: 0000-0002-8241-9520),从事园林繁殖栽培与生物技术研究。E-mail: 1012042154@qq.com
    通信作者: 文书生(ORCID: 0000-0003-0983-6755),讲师,从事园林繁殖栽培与生物技术研究。E-mail: shusheng0507@126.com
  • 中图分类号: S718.3

Research advances in the mechanism of adventitious root occurrence in woody species

  • 摘要: 不定根是植物的茎或叶等非中柱鞘组织产生的根,不定根发生困难是诸多木本植物无性繁殖和工厂化育苗的瓶颈问题,然而相关机制尚不明晰。目前,对于木本植物不定根发生机理的研究主要包括3个方面:①根原基的形成是不定根发生的关键,利用石蜡切片技术对根原基的形成时间和部位进行观察,同时依据组织形态观察结果将不定根发生过程划分为3个主要时期。②不定根发生是一个复杂的生理生化过程,内源激素含量和生根关联酶活性的动态变化在不定根发生过程中发挥着重要调控作用。此外,营养物质、酚类物质以及多胺等物质也被认为是影响不定根发生的重要因素。③探究了部分木本植物不定根发生过程中的关键代谢通路,并挖掘出多个调控不定根发生的基因,鉴定出诸多参与不定根发生的转录因子和非编码的微小核糖核酸(microRNA)。综上,本研究系统概述了木本植物不定根发生的组织学、生理学和分子调控机理的研究进展,并展望了未来该领域的可行性研究方向,为进一步探究木本植物不定根发生机制提供参考。表1参80
  • 表  1  木本植物不定根发生相关调控因子

    Table  1.   Regulation factors related to adventitious root in woody species

    类型名称描述植物研究结果参考文献
    基因 ARRO-1 木本植物不定根发生的分子 标记之一 苹果、小金海棠、牡丹‘乌龙捧盛’ 其表达与根原基的形成密切相关, 调控内源生长素的动态平衡 [6466]
    LRP1 不定根原基分化早期阶段的 分子标记之一 核桃、南林895杨 根原基发端组织中特异性表达,根 原基形成阶段强烈表达 [67]
    PRP1,2 编码脯氨酸富含蛋白 葡萄 脱分化期的薄壁组织中优先表达且 水平高,改变细胞壁特性 [68]
    YUCCA 编码IAA生物合成限速酶的 基因之一 84K杨 缩短根形成的时间,促进根系发育, 促进形成层区域分生细胞的分裂 [5]
    TIR1 编码生长素受体转运抑制应 答因子 鲁桑 诱导期与分化期迅速上调表达,参 与调控不定根原基的产生与发育 [69]
    GH3家族 生长素早期响应基因家族 鲁桑 GH3.1与ILL5共同调控内源生长素 浓度,促进不定根发生 [70]
    PIN家族 编码生长素极性运输所依赖 的转运蛋白 芒果 调控组织内生长素富集,从而促进 其不定根发生 [71]
    转录因子家族 AP2/EREBP 调控植物逆境应答和生长发 育的信号转导 毛果杨 促进根原基的形成,增加不定根数 量,缩短根形成时间 [72]
    ARFs 调控生长素信号转导 苹果、油橄榄、杜仲 调控不定根发生过程中的生长素
     水平
    [73]
    GRAs 维持分生组织的分生活性、 影响根的发育和调控激素 信号转导 欧洲栗、辐射松、核桃 促进根原基的形成 [6, 74]
    LBD 调控愈伤组织的形成 84K杨 促使愈伤组织过度膨大,植株不定 根数量减少,根长缩短 [75]
    MYB 参与植物次生代谢、细胞分 化、抗逆反应 鲁桑 促进插穗薄壁细胞的分化 [76]
    NAC 参与侧根发育、根尖分生组 织发育、维管木质化、非 生物胁迫和防卫反应 南林895杨 参与不定根原基的启动诱导和侧根 的发育 [67]
    WOX 维持根尖维管分生组织中干 细胞活性和调控生根过程 激素信号 日本落叶松、84K杨 调控生长素、茉莉酸、脱落酸信号 通路,影响生长素的极性运输 [77]
    WRKY 参与防卫反应、机械创伤修 复和生长发育 南林895杨 参与插穗的创伤修复和防卫反应以 及植物系统获得性抗性防御或者 响应环境胁迫 [67]
    microRNA mdm-miR156 调控植物次生代谢、光信号 转导和胁迫响应 小金海棠 下调转录因子MxSPL26的水平,促 进不定根原基的启动和发育良  好,提高生根率与生根速度 [7]
    mdm-miR160 靶向生长素响应因子    (ARF)家族 圆叶海棠 靶向调控MdARF16和MdARF17的 表达,抑制不定根发生 [78]
    miR396 负调控生长调节因子(GRF) 苹果 靶向调控MdGRF1和MdGRF5的表 达,参与不定根诱导期和根伸长 期的发育 [79]
    miR319a 调控激素合成 毛白杨 靶向调控TCP19的表达,影响生长 素信号转导,负调控不定根发生 [80]
      说明:小金海棠 Malus xiaojinensis;牡丹‘乌龙捧盛’ Paeonia suffruticosa ‘Wulongpengsheng’;葡萄 Vitis vinifera;84K 杨      Populus alba × Populus glandulosa;鲁桑 Morus multicaulis;芒果 Mangifera indica;毛果杨 Populus trichocarpa;油橄榄 Olea      europaea;杜仲 Eucommia ulmoides;欧洲栗 Pinus radiata;辐射松 Castanea sativa;日本落叶松 Larix kaempferi;圆叶海棠      Malus prunifolia var. ringo
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-09-22
  • 录用日期:  2022-05-13
  • 修回日期:  2022-04-25

木本植物不定根发生机制研究进展

doi: 10.11833/j.issn.2095-0756.20210652
    基金项目:  国家自然科学基金资助项目(32001359);江苏省自然科学基金资助项目(BK20180771);江苏高校优势学科建设工程资助项目(PAPD);南京林业大学青年科技创新基金(CX2018026);南京林业大学大学生创新训练计划项目(2021NFUSPITP0015)
    作者简介:

    苗大鹏(ORCID: 0000-0002-8241-9520),从事园林繁殖栽培与生物技术研究。E-mail: 1012042154@qq.com

    通信作者: 文书生(ORCID: 0000-0003-0983-6755),讲师,从事园林繁殖栽培与生物技术研究。E-mail: shusheng0507@126.com
  • 中图分类号: S718.3

摘要: 不定根是植物的茎或叶等非中柱鞘组织产生的根,不定根发生困难是诸多木本植物无性繁殖和工厂化育苗的瓶颈问题,然而相关机制尚不明晰。目前,对于木本植物不定根发生机理的研究主要包括3个方面:①根原基的形成是不定根发生的关键,利用石蜡切片技术对根原基的形成时间和部位进行观察,同时依据组织形态观察结果将不定根发生过程划分为3个主要时期。②不定根发生是一个复杂的生理生化过程,内源激素含量和生根关联酶活性的动态变化在不定根发生过程中发挥着重要调控作用。此外,营养物质、酚类物质以及多胺等物质也被认为是影响不定根发生的重要因素。③探究了部分木本植物不定根发生过程中的关键代谢通路,并挖掘出多个调控不定根发生的基因,鉴定出诸多参与不定根发生的转录因子和非编码的微小核糖核酸(microRNA)。综上,本研究系统概述了木本植物不定根发生的组织学、生理学和分子调控机理的研究进展,并展望了未来该领域的可行性研究方向,为进一步探究木本植物不定根发生机制提供参考。表1参80

English Abstract

苗大鹏, 贾瑞瑞, 李胜皓, 席烁, 朱葛, 文书生. 木本植物不定根发生机制研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210652
引用本文: 苗大鹏, 贾瑞瑞, 李胜皓, 席烁, 朱葛, 文书生. 木本植物不定根发生机制研究进展[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20210652
MIAO Dapeng, JIA Ruirui, LI Shenghao, XI Shuo, ZHU Ge, WEN Shusheng. Research advances in the mechanism of adventitious root occurrence in woody species[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210652
Citation: MIAO Dapeng, JIA Ruirui, LI Shenghao, XI Shuo, ZHU Ge, WEN Shusheng. Research advances in the mechanism of adventitious root occurrence in woody species[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210652

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