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毛竹Ph-TElncRNA1的鉴定及对靶基因的调控

赵佳敏 余璐 丁一倩 周明兵

赵佳敏, 余璐, 丁一倩, 周明兵. 毛竹Ph-TElncRNA1的鉴定及对靶基因的调控[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20220396
引用本文: 赵佳敏, 余璐, 丁一倩, 周明兵. 毛竹Ph-TElncRNA1的鉴定及对靶基因的调控[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20220396
ZHAO Jiamin, YU Lu, DING Yiqian, ZHOU Mingbing. Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20220396
Citation: ZHAO Jiamin, YU Lu, DING Yiqian, ZHOU Mingbing. Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20220396

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毛竹Ph-TElncRNA1的鉴定及对靶基因的调控

doi: 10.11833/j.issn.2095-0756.20220396
基金项目: 国家自然科学基金资助项目(31870656);浙江自然科学基金重点资助项目(LZ19C160001)
详细信息
    作者简介: 赵佳敏(ORCID: 0000-0002-7894-4787),从事生物学研究。E-mail: 1160273180@qq.com
    通信作者: 周明兵(ORCID: 0000-0001-5674-4410),教授,博士,从事竹类植物基因组进化研究。E-mail: zhoumingbing@zafu.edu.cn
  • 中图分类号: Q943.2;S722.3

Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes

  • 摘要:   目的   旨在探究毛竹Phyllostachys edulis Ph-TElncRNA1及靶基因的表达模式,初步分析lncRNA的功能。   方法   基于高温、低温、紫外、高盐胁迫处理毛竹实生苗全转录组测序数据,筛选在胁迫下差异表达的lncRNA。通过CNCI、Pfam、CPC2、PLEK等4个软件对lncRNA的编码特性进行分析,用LncRNATar软件鉴定lncRNA的靶基因,通过实时荧光定量PCR分析lncRNA和靶基因在紫外胁迫下和叶片着色过程中的表达模式。   结果   筛选到1个在紫外胁迫下差异表达的lncRNA,此lncRNA来源于LARD转座子序列(large retrotransposons derivatives),命名为Ph-TElncRNA1(Phyllostachys edulis transposable element derived lncRNA1)。Ph-TElncRNA1是一个典型的非编码RNA,全长为342 bp,其中,185个碱基来源于外显子,157个碱基来源于内含子。Ph-TElncRNA1的靶基因为psbA,编码photosystem Ⅱprotein D1。实时荧光定量结果表明:Ph-TElncRNA1与psbA的相对表达量呈完全相同的趋势,说明在紫外胁迫下Ph-TElncRNA1与psbA呈正相关。通过Ph-TElncRNA1和psbA在不同着色时期的毛竹叶片的相对表达量分析,发现在叶绿体形成期,Ph-TElncRNA1和psbA表达量达到峰值。   结论   Ph-TElncRNA1和psbA在紫外胁迫下协同表达,Ph-TElncRNA1可以通过调控靶基因psbA参与毛竹叶片发育。图7参33
  • 图  1  黄色、淡黄、淡绿、绿色4种颜色的叶片

    Figure  1  Leaves with yellow, light yellow, light green and green color

    图  2  转座子LARD和Ph-TElncRNA1位置关系结构图

    Figure  2  2Positional relationship between the LARD transposon and Ph-TElncRNA1

    图  3  Ph-TElncRNA1的组成结构及序列

    Figure  3  Composition and sequence of Ph-TElncRNA1

    图  4  Ph-TElncRNA1的电泳图

    Figure  4  Electropherogram of Ph-TElncRNA1

    图  5  Ph-TElncRNA1的测序结果比对

    Figure  5  Alignment of sequencing results of Ph-TElncRNA1

    图  6  Ph-TElncRNA1与psbA在紫外胁迫处理下的相对表达量

    Figure  6  Relative expression of Ph-TElncRNA1and psbA under UV treatment    

    图  7  Ph-TElncRNA1与psbA在叶片着色4个时期的相对表达量

    Figure  7  Relative expression of Ph-TElncRNA1and psbA in four periods of leaves colorization

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  • 文章访问数:  15
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出版历程
  • 收稿日期:  2022-06-14
  • 录用日期:  2022-10-17
  • 修回日期:  2022-10-10

毛竹Ph-TElncRNA1的鉴定及对靶基因的调控

doi: 10.11833/j.issn.2095-0756.20220396
    基金项目:  国家自然科学基金资助项目(31870656);浙江自然科学基金重点资助项目(LZ19C160001)
    作者简介:

    赵佳敏(ORCID: 0000-0002-7894-4787),从事生物学研究。E-mail: 1160273180@qq.com

    通信作者: 周明兵(ORCID: 0000-0001-5674-4410),教授,博士,从事竹类植物基因组进化研究。E-mail: zhoumingbing@zafu.edu.cn
  • 中图分类号: Q943.2;S722.3

摘要:    目的   旨在探究毛竹Phyllostachys edulis Ph-TElncRNA1及靶基因的表达模式,初步分析lncRNA的功能。   方法   基于高温、低温、紫外、高盐胁迫处理毛竹实生苗全转录组测序数据,筛选在胁迫下差异表达的lncRNA。通过CNCI、Pfam、CPC2、PLEK等4个软件对lncRNA的编码特性进行分析,用LncRNATar软件鉴定lncRNA的靶基因,通过实时荧光定量PCR分析lncRNA和靶基因在紫外胁迫下和叶片着色过程中的表达模式。   结果   筛选到1个在紫外胁迫下差异表达的lncRNA,此lncRNA来源于LARD转座子序列(large retrotransposons derivatives),命名为Ph-TElncRNA1(Phyllostachys edulis transposable element derived lncRNA1)。Ph-TElncRNA1是一个典型的非编码RNA,全长为342 bp,其中,185个碱基来源于外显子,157个碱基来源于内含子。Ph-TElncRNA1的靶基因为psbA,编码photosystem Ⅱprotein D1。实时荧光定量结果表明:Ph-TElncRNA1与psbA的相对表达量呈完全相同的趋势,说明在紫外胁迫下Ph-TElncRNA1与psbA呈正相关。通过Ph-TElncRNA1和psbA在不同着色时期的毛竹叶片的相对表达量分析,发现在叶绿体形成期,Ph-TElncRNA1和psbA表达量达到峰值。   结论   Ph-TElncRNA1和psbA在紫外胁迫下协同表达,Ph-TElncRNA1可以通过调控靶基因psbA参与毛竹叶片发育。图7参33

English Abstract

赵佳敏, 余璐, 丁一倩, 周明兵. 毛竹Ph-TElncRNA1的鉴定及对靶基因的调控[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20220396
引用本文: 赵佳敏, 余璐, 丁一倩, 周明兵. 毛竹Ph-TElncRNA1的鉴定及对靶基因的调控[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20220396
ZHAO Jiamin, YU Lu, DING Yiqian, ZHOU Mingbing. Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20220396
Citation: ZHAO Jiamin, YU Lu, DING Yiqian, ZHOU Mingbing. Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20220396

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