Identification of Ph-TElncRNA1 in Phyllostachys edulis and its regulation of target genes
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摘要:
目的 旨在探究毛竹Phyllostachys edulis Ph-TElncRNA1及靶基因的表达模式，初步分析lncRNA的功能。 方法 基于高温、低温、紫外、高盐胁迫处理毛竹实生苗全转录组测序数据，筛选在胁迫下差异表达的lncRNA。通过CNCI、Pfam、CPC2、PLEK等4个软件对lncRNA的编码特性进行分析，用LncRNATar软件鉴定lncRNA的靶基因，通过实时荧光定量PCR分析lncRNA和靶基因在紫外胁迫下和叶片着色过程中的表达模式。 结果 筛选到1个在紫外胁迫下差异表达的lncRNA，此lncRNA来源于LARD转座子序列(large retrotransposons derivatives)，命名为Ph-TElncRNA1(Phyllostachys edulis transposable element derived lncRNA1)。Ph-TElncRNA1是一个典型的非编码RNA，全长为342 bp，其中，185个碱基来源于外显子，157个碱基来源于内含子。Ph-TElncRNA1的靶基因为psbA，编码photosystem Ⅱprotein D1。实时荧光定量结果表明：Ph-TElncRNA1与psbA的相对表达量呈完全相同的趋势，说明在紫外胁迫下Ph-TElncRNA1与psbA呈正相关。通过Ph-TElncRNA1和psbA在不同着色时期的毛竹叶片的相对表达量分析，发现在叶绿体形成期，Ph-TElncRNA1和psbA表达量达到峰值。 结论 Ph-TElncRNA1和psbA在紫外胁迫下协同表达，Ph-TElncRNA1可以通过调控靶基因psbA参与毛竹叶片发育。图7参33 -
关键词:
- Ph-TElncRNA1 /
- 叶片着色 /
- D1蛋白 /
- 毛竹
Abstract:Objective The purpose of this study is to explore the expression patterns of Ph-TElncRNA1 and target genes in Phyllostachys edulis, and to preliminarily analyze the function of lncRNA. Method Based on the whole transcriptome sequencing data of Ph. edulis seedlings under high temperature, low temperature, ultraviolet and high salt stress, lncRNAs differentially expressed under stress were screened. The coding characteristics of lncRNA were analyzed by four kinds of software(CNCI, Pfam, CPC2 and PLEK), and the target genes of lncRNA were identified by LncTar software. The expression patterns of lncRNA and target genes under UV stress and leaf coloration were analyzed by real-time fluorescent quantitative PCR. Result One differentially expressed lncRNA under UV stress was screened, which was derived from the lncRNA of the Large retrotransposons derivatives and named Ph-TElncRNA1(Ph. edulis transposable element derived lncRNA1). Ph-TElncRNA1 was a typical non-coding RNA with a total length of 342 bp, 185 bases from exons and 157 bases from introns. The target gene of Ph-TElncRNA1 was psbA, encoding photosystem Ⅱ protein D1. Real time fluorescence quantitative results showed that the relative expression of Ph-TElncRNA1 and psbA was the same, indicating that Ph-TElncRNA1 was positively correlated with psbA under UV stress. Through the analysis of the relative expression of Ph-TElncRNA1 and psbA in Ph. edulis leavesat different coloring stages, it was found that the expression of psbA and Ph-TElncRNA1 reached the peak during chloroplast formation stage. Conclusion Ph-TElncRNA1 and psbA are co-expressed under UV stress, andPh-TElncRNA1 can participate in the leaf development of Ph. edulis by regulating the target gene psbA.[Ch, 7 fig. 33 ref.] -
Key words:
- Ph-TElncRNA1 /
- leaf coloration /
- D1 protein /
- Phyllostachys edulis
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图 1 黄色、淡黄、淡绿、绿色4种颜色的叶片
Figure 1 Leaves with yellow, light yellow, light green and green color
图 2 转座子LARD和Ph-TElncRNA1位置关系结构图
Figure 2 2Positional relationship between the LARD transposon and Ph-TElncRNA1
图 6 Ph-TElncRNA1与psbA在紫外胁迫处理下的相对表达量
Figure 6 Relative expression of Ph-TElncRNA1and psbA under UV treatment
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