Volume 28 Issue 4
Aug.  2011
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LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022
Citation: LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022
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  • Corresponding author: KANG Hua-jing
  • Publish Date: 2011-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022
    Corresponding author: KANG Hua-jing

Abstract: Hemsleya zhejiangensis is an endemic species in China, its population of H. zhejiangensis is declining due to the lack of seedlings and the resource volume should expanded to sustain the population exist. The tissue culture was of H. zhejiangensis maily disccused in this paper. The effects of two hormones:6-benzyladenine(6-BA) and Naphthalene acetic acid (NAA),and four types of culture media: Murashige and Skoog (MS),Gamborg (B5),N6-benzylaminopurine(N6) and woody plant medium (WPM) on the propagation of H. zhejiangensis were studied. The results were all meansSD of fourty independent experiments. Results showed the optimum concentration (for 6-BA) was 0.50 mgL-1 and (for NAA) was 0.20 mgL-1 with propagation coefficients of 10.80 (6-BA) and 5.60 (NAA). When both hormones were used together,treatment (1) of MS + 6-BA 1.00 mgL-1 + NAA 0.02 mgL-1 and treatment(2) of MS + 6-BA 1.00 mgL-1 + NAA 0.05 mgL-1 were much better,which propagation coefficients of 19.30 (for treatment 1) and 18.10 (for treatment 2). [Ch,1 fig. 4 tab. 7 ref.]

LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022
Citation: LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022

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