<em>Hemsleya zhejiangensis</em> seedling culture with two hormones and four types of medium

LEI Zu-pei; YU Hong-ao; ZHANG Shu-run; LIN Rui-feng; KANG Hua-jing

doi:10.11833/j.issn.2095-0756.2011.04.022

Volume 28 Issue 4

Aug. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(4): 662-666

LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022

Citation:

LEI Zu-pei, YU Hong-ao, ZHANG Shu-run, LIN Rui-feng, KANG Hua-jing. Hemsleya zhejiangensis seedling culture with two hormones and four types of medium[J]. Journal of Zhejiang A&F University, 2011, 28(4): 662-666. doi: 10.11833/j.issn.2095-0756.2011.04.022

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022

1.
Management Office，Wuyanlin Nature Reserve of Zhejiang，Taishun 325500，Zhejiang，China;
2.
Department of Landscape Architecture，The Science and Technology College of Wenzhou，Wenzhou 325006，Zhejiang，China

More Information

Corresponding author: KANG Hua-jing
Publish Date: 2011-08-20

Abstract

Hemsleya zhejiangensis is an endemic species in China, its population of H. zhejiangensis is declining due to the lack of seedlings and the resource volume should expanded to sustain the population exist. The tissue culture was of H. zhejiangensis maily disccused in this paper. The effects of two hormones：6-benzyladenine（6-BA） and Naphthalene acetic acid （NAA），and four types of culture media: Murashige and Skoog （MS），Gamborg （B5），N6-benzylaminopurine（N6） and woody plant medium （WPM） on the propagation of H. zhejiangensis were studied. The results were all meansSD of fourty independent experiments. Results showed the optimum concentration （for 6-BA） was 0.50 mgL-1 and （for NAA） was 0.20 mgL-1 with propagation coefficients of 10.80 （6-BA） and 5.60 （NAA）. When both hormones were used together，treatment （1） of MS + 6-BA 1.00 mgL-1 + NAA 0.02 mgL-1 and treatment（2） of MS + 6-BA 1.00 mgL-1 + NAA 0.05 mgL-1 were much better，which propagation coefficients of 19.30 （for treatment 1） and 18.10 （for treatment 2）. ［Ch，1 fig. 4 tab. 7 ref.］
- botany,
- Hemsleya zhejiangensis,
- tissue culture,
- plant growth regulating substances,
- culture medium,
- propagation coefficient

Relative Article

[1]	ZHOU Ling, WANG Xiaoqin, LIN Xinchun. In vitro micropropagation of Gigantochloa tekserah . Journal of Zhejiang A&F University, 2014, 31(5): 817-820. doi: 10.11833/j.issn.2095-0756.2014.05.025
[2]	RUAN Huize, LI Zhen, REN Yanyan, WU Xiaonan, SHAO Yuhao, PAN Feixiang, XIA Guohua. Plantlet regeneration of Hemiboea subcapitata with subculturing . Journal of Zhejiang A&F University, 2014, 31(1): 162-166. doi: 10.11833/j.issn.2095-0756.2014.01.025
[3]	ZHOU Yanqing, DING Lan, XU Buqing, XIA Guohua, CUI Yongyi. Tissue culture and rapid propagation of Ficus pandurata var.angustifolia with different plant growth regulators . Journal of Zhejiang A&F University, 2013, 30(3): 453-458. doi: 10.11833/j.issn.2095-0756.2013.03.024
[4]	CHEN Jialong, ZHU Jianjun, WU Xiushui, YU Hong′ao. Tissue culture and rapid propagation of Caragana sinica . Journal of Zhejiang A&F University, 2013, 30(4): 611-614. doi: 10.11833/j.issn.2095-0756.2013.04.022
[5]	HANG Lu-lu, ZHANG Nan, JI Meng-cheng. Haplocladium microphyllum spore germination with different culture mediums and sucrose concentrations . Journal of Zhejiang A&F University, 2012, 29(3): 383-387. doi: 10.11833/j.issn.2095-0756.2012.03.009
[6]	CHENG Ying, LI Gen-you, XIA Guo-hua, HUANG Shang-jue, HUANG Yu-feng. Review on tissue culture of Aralia plants . Journal of Zhejiang A&F University, 2011, 28(6): 968-972. doi: 10.11833/j.issn.2095-0756.2011.06.022
[7]	ZHANG Nan, DU Bao-ming, JI Meng-cheng. Research progress on the bryophytes tissue culture . Journal of Zhejiang A&F University, 2011, 28(2): 305-313. doi: 10.11833/j.issn.2095-0756.2011.02.022
[8]	XU Yong, SUN Jun-wei, CHEN Zhen. Plant growth regulator treatments for a tissue culture of Euodia rutaecarpa . Journal of Zhejiang A&F University, 2011, 28(3): 500-504. doi: 10.11833/j.issn.2095-0756.2011.03.024
[9]	TAN Xiao－feng, YUAN De－yi, YUAN Jun, LIAO Ting. Pollen germination in Camellia oleifera with ascorbic acid and plant growth regulators . Journal of Zhejiang A&F University, 2010, 27(6): 941-944. doi: 10.11833/j.issn.2095-0756.2010.06.023
[10]	WANG Hui, LOU Lu-huan, ZHU Xiao-lou. Effects of exogenous hormones on rooting of Euonymus hamiltonianus and E. alatus cuttings . Journal of Zhejiang A&F University, 2010, 27(1): 155-158. doi: 10.11833/j.issn.2095-0756.2010.01.025
[11]	YANG Hai-yun, GUI Ren-yi, TANG Ding-qin, FANG Wei. Micropropagation of Sasa fortunei . Journal of Zhejiang A&F University, 2008, 25(2): 255-258.
[12]	PENG Shao-bing, WANG De-xiang. Tissue culture and rapid propagation of Atriplex canescens . Journal of Zhejiang A&F University, 2007, 24(3): 377-381.
[13]	AI Jian-guo, YU Lin, ZHANG Li-ying, QIAN Liu-qin, ZHANG Teng-chao. Plant community properties in a Hemsleya zhejiangensis habitat in Wuyanling Nature Reserve, Zhejiang Province . Journal of Zhejiang A&F University, 2007, 24(6): 706-710.
[14]	XU Feng-lan, YANG Lun-zeng. Effects of plant growth regulators on vegetative growth of Castanea henryi . Journal of Zhejiang A&F University, 2006, 23(4): 410-413.
[15]	GAO Yan-hui, TONG Zai-kang, HUANG Hua-hong, GUO Fu-qi, YU Chong-yan. Tissue culture on Trachelospermum jasminoides `Variegatum' . Journal of Zhejiang A&F University, 2006, 23(6): 701-704.
[16]	LIU Zhi-gao, CHU Jia-miao, GAO Yan-hui, ZHANG Lu, . Tissue culture in vitro of Lycoris albiflora . Journal of Zhejiang A&F University, 2006, 23(3): 347-350.
[17]	ZHU Yu-qiu, HUANG Hua-hong, LU Hai-gen, TONG Zai-kang. Rapid propagation of Photinia glabra by tissue culture . Journal of Zhejiang A&F University, 2005, 22(2): 238-240.
[18]	WU Yue-yan, TAO Wei-fang. Tissue culture and propagation in grape . Journal of Zhejiang A&F University, 2001, 18(2): 188-192.
[19]	Zhang Liqin, Zheng Yong ping, Luo Shiyuan, Hu Jiagong.. Salt Tolerance of Calli of Populus Clones and Pinus elliottii in Tissue Cultures . Journal of Zhejiang A&F University, 1997, 14(1): 16-21.
[20]	Zhang Liqin, Zheng YongPing, Jin Peiying. selection of Salt Tolerance poplar Regenerated plant-lets in Tissues Culture. . Journal of Zhejiang A&F University, 1996, 13(4): 397-404.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(5243) PDF downloads(1354) Cited by()

Proportional views

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

1. Management Office，Wuyanlin Nature Reserve of Zhejiang，Taishun 325500，Zhejiang，China;

2. Department of Landscape Architecture，The Science and Technology College of Wenzhou，Wenzhou 325006，Zhejiang，China

Corresponding author: KANG Hua-jing

Publish Date: 2011-08-20

Key words:

Abstract: Hemsleya zhejiangensis is an endemic species in China, its population of H. zhejiangensis is declining due to the lack of seedlings and the resource volume should expanded to sustain the population exist. The tissue culture was of H. zhejiangensis maily disccused in this paper. The effects of two hormones：6-benzyladenine（6-BA） and Naphthalene acetic acid （NAA），and four types of culture media: Murashige and Skoog （MS），Gamborg （B5），N6-benzylaminopurine（N6） and woody plant medium （WPM） on the propagation of H. zhejiangensis were studied. The results were all meansSD of fourty independent experiments. Results showed the optimum concentration （for 6-BA） was 0.50 mgL-1 and （for NAA） was 0.20 mgL-1 with propagation coefficients of 10.80 （6-BA） and 5.60 （NAA）. When both hormones were used together，treatment （1） of MS + 6-BA 1.00 mgL-1 + NAA 0.02 mgL-1 and treatment（2） of MS + 6-BA 1.00 mgL-1 + NAA 0.05 mgL-1 were much better，which propagation coefficients of 19.30 （for treatment 1） and 18.10 （for treatment 2）. ［Ch，1 fig. 4 tab. 7 ref.］

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022

1. Management Office，Wuyanlin Nature Reserve of Zhejiang，Taishun 325500，Zhejiang，China;

2. Department of Landscape Architecture，The Science and Technology College of Wenzhou，Wenzhou 325006，Zhejiang，China

Corresponding author: KANG Hua-jing

HTML

Catalog

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Hemsleya zhejiangensis seedling culture with two hormones and four types of medium

doi: 10.11833/j.issn.2095-0756.2011.04.022

1. Management Office，Wuyanlin Nature Reserve of Zhejiang，Taishun 325500，Zhejiang，China; 2. Department of Landscape Architecture，The Science and Technology College of Wenzhou，Wenzhou 325006，Zhejiang，China

Corresponding author: KANG Hua-jing

HTML

Catalog

Export File

Citation

Format

Content

1. Management Office，Wuyanlin Nature Reserve of Zhejiang，Taishun 325500，Zhejiang，China;

2. Department of Landscape Architecture，The Science and Technology College of Wenzhou，Wenzhou 325006，Zhejiang，China