Volume 28 Issue 5
Oct.  2011
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GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023
Citation: GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023

RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023
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  • Corresponding author: TONG Zai-kang
  • Publish Date: 2011-10-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023
    Corresponding author: TONG Zai-kang

Abstract: In order to protect germplasm resources of endangered plant:Pseudolarix amabilis,genetic diversity of Pseudolarix amabilis from different sources in Zhejiang Province were analyzed using an optimal protocol of random amplified polymorphism DNA(RAPD) followed by a cluster analysis in this study. Results showed that for 62 individuals with 18 random primers,172 repetitive loci were detected,among which 170 were polymorphic for a species level polymorphic loci rate of 99.2%. The cluster analysis showed that materials from the same sources were classified together. Thus,RAPD polymorphism classification for natural populations of P. amabilis was mostly related to geographic distribution, genetic diversity was high among the P. amabilis population,and both in situ preservation and ex-situ conservation were possible. [Ch,2 fig. 3 tab. 31 ref.]

GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023
Citation: GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis: a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023

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