RAPD analysis of genetic diversity for <em>Pseudolarix amabilis</em>： a critically endangered plant

GAO Yan-hui; FAN Min-liang; LUO Wen-jian; HUANG Hua-hong; TONG Zai-kang

doi:10.11833/j.issn.2095-0756.2011.05.023

Volume 28 Issue 5

Oct. 2011

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GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023

Citation:

GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant[J]. Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023

RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023

1.
Research Institute of Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
Forest Tree Seed and Stock Master Station of Zhejiang Province，Hangzhou 310020，Zhejiang，China

More Information

Corresponding author: TONG Zai-kang
Publish Date: 2011-10-20

Abstract

In order to protect germplasm resources of endangered plant：Pseudolarix amabilis，genetic diversity of Pseudolarix amabilis from different sources in Zhejiang Province were analyzed using an optimal protocol of random amplified polymorphism DNA（RAPD） followed by a cluster analysis in this study. Results showed that for 62 individuals with 18 random primers，172 repetitive loci were detected，among which 170 were polymorphic for a species level polymorphic loci rate of 99.2%. The cluster analysis showed that materials from the same sources were classified together. Thus，RAPD polymorphism classification for natural populations of P. amabilis was mostly related to geographic distribution, genetic diversity was high among the P. amabilis population，and both in situ preservation and ex-situ conservation were possible. ［Ch，2 fig. 3 tab. 31 ref.］
- forest tree breeding,
- Pseudolarix amabilis,
- genetic diversity,
- random amplified polymorphism DNA,
- ex-situ conservation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

1. Research Institute of Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Forest Tree Seed and Stock Master Station of Zhejiang Province，Hangzhou 310020，Zhejiang，China

Corresponding author: TONG Zai-kang

Publish Date: 2011-10-20

Key words:

Abstract: In order to protect germplasm resources of endangered plant：Pseudolarix amabilis，genetic diversity of Pseudolarix amabilis from different sources in Zhejiang Province were analyzed using an optimal protocol of random amplified polymorphism DNA（RAPD） followed by a cluster analysis in this study. Results showed that for 62 individuals with 18 random primers，172 repetitive loci were detected，among which 170 were polymorphic for a species level polymorphic loci rate of 99.2%. The cluster analysis showed that materials from the same sources were classified together. Thus，RAPD polymorphism classification for natural populations of P. amabilis was mostly related to geographic distribution, genetic diversity was high among the P. amabilis population，and both in situ preservation and ex-situ conservation were possible. ［Ch，2 fig. 3 tab. 31 ref.］

RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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Proportional views

RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023

1. Research Institute of Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Forest Tree Seed and Stock Master Station of Zhejiang Province，Hangzhou 310020，Zhejiang，China

Corresponding author: TONG Zai-kang

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RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023

Abstract

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References

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通讯作者: 陈斌, bchen63@163.com

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RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant

doi: 10.11833/j.issn.2095-0756.2011.05.023

1. Research Institute of Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. Forest Tree Seed and Stock Master Station of Zhejiang Province，Hangzhou 310020，Zhejiang，China

Corresponding author: TONG Zai-kang

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1. Research Institute of Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. Forest Tree Seed and Stock Master Station of Zhejiang Province，Hangzhou 310020，Zhejiang，China