Cloning and sequence analysis of fruit ripening-specific gene <em>CsPMEI</em>/<em>InvI</em> from citrus

WANG Guoli; AN Huaming; QIN Qiaoping; LI Mengjiao; LIU Zhenzhen; CHEN Jiaying; ZHOU Qian; ZHANG Lanlan

doi:10.11833/j.issn.2095-0756.2013.03.005

Volume 30 Issue 3

May 2013

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Article Navigation > Journal of Zhejiang A&F University > 2013 > 30(3): 336-342

WANG Guoli, AN Huaming, QIN Qiaoping, LI Mengjiao, LIU Zhenzhen, CHEN Jiaying, ZHOU Qian, ZHANG Lanlan. Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus[J]. Journal of Zhejiang A&F University, 2013, 30(3): 336-342. doi: 10.11833/j.issn.2095-0756.2013.03.005

Citation:

WANG Guoli, AN Huaming, QIN Qiaoping, LI Mengjiao, LIU Zhenzhen, CHEN Jiaying, ZHOU Qian, ZHANG Lanlan. Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus[J]. Journal of Zhejiang A&F University, 2013, 30(3): 336-342. doi: 10.11833/j.issn.2095-0756.2013.03.005

Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

doi: 10.11833/j.issn.2095-0756.2013.03.005

1.
Guizhou Fruits Engineering Technology Research Centre，Guiyang 550025，Guizhou，China;
2.
School of Agriculture，Guizhou University，Guiyang 550025，Guizhou，China;
3.
The Key Laboratory for Quality Improvement of Agricultural Products of Zhlejiang Province， School of Agriculture and Food Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

More Information

Corresponding author: ZHANG Lanlan
Received Date: 2012-12-26
Rev Recd Date: 2013-01-19
Publish Date: 2013-06-20

Abstract

Fruit-ripening-specific expression genes play important roles on the regulation of fruit ripening and quality formation. A full-length cDNA of fruit ripening-specific gene，designated as CsPEMI/InvI （GenBank accession No. KC198084），was isolated from Newhall navel orange （Citrus sinensis Newhall） by RT-PCR and RACE. The full-length of CsPMEI/InvI was 945 bp，containing an open reading frame of 618 bp and encoding 205 amino acids. The molecular formula of encoded protein was C977H1568N296O282S10 with a calculated molecular of 22.29 kDa and an isoelectric point of 9.84. The encoded protein belonged to InvI/PMEI family including highly-conserved Cys residue in the protein sequence，one cAMP and cGMP-dependent protein kinase phosphorylation site，three protein kinase C phosphorylation sites，two casein kinase Ⅱ phosphorylation sites and three N-myristoylation sites. In its secondary structure，-helix was the main structural conformation. ［Ch，7 fig. 1 tab. 28 ref.］
- horticulture,
- ripening-specific,
- PMEI/InvI,
- gene cloning,
- sequence analysis,
- citrus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

1. Guizhou Fruits Engineering Technology Research Centre，Guiyang 550025，Guizhou，China;

2. School of Agriculture，Guizhou University，Guiyang 550025，Guizhou，China;

3. The Key Laboratory for Quality Improvement of Agricultural Products of Zhlejiang Province， School of Agriculture and Food Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China

Corresponding author: ZHANG Lanlan

Received Date: 2012-12-26

Rev Recd Date: 2013-01-19

Publish Date: 2013-06-20

Key words:

Abstract: Fruit-ripening-specific expression genes play important roles on the regulation of fruit ripening and quality formation. A full-length cDNA of fruit ripening-specific gene，designated as CsPEMI/InvI （GenBank accession No. KC198084），was isolated from Newhall navel orange （Citrus sinensis Newhall） by RT-PCR and RACE. The full-length of CsPMEI/InvI was 945 bp，containing an open reading frame of 618 bp and encoding 205 amino acids. The molecular formula of encoded protein was C977H1568N296O282S10 with a calculated molecular of 22.29 kDa and an isoelectric point of 9.84. The encoded protein belonged to InvI/PMEI family including highly-conserved Cys residue in the protein sequence，one cAMP and cGMP-dependent protein kinase phosphorylation site，three protein kinase C phosphorylation sites，two casein kinase Ⅱ phosphorylation sites and three N-myristoylation sites. In its secondary structure，-helix was the main structural conformation. ［Ch，7 fig. 1 tab. 28 ref.］

Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

doi: 10.11833/j.issn.2095-0756.2013.03.005

Abstract

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Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

doi: 10.11833/j.issn.2095-0756.2013.03.005

Corresponding author: ZHANG Lanlan

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Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

doi: 10.11833/j.issn.2095-0756.2013.03.005

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Cloning and sequence analysis of fruit ripening-specific gene CsPMEI/InvI from citrus

doi: 10.11833/j.issn.2095-0756.2013.03.005

Corresponding author: ZHANG Lanlan

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