Volume 30 Issue 4
Jul.  2013
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RUAN Jiajia, YUAN Jinqiang, YANG Xianyu. Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus[J]. Journal of Zhejiang A&F University, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016
Citation: RUAN Jiajia, YUAN Jinqiang, YANG Xianyu. Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus[J]. Journal of Zhejiang A&F University, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016

Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus

doi: 10.11833/j.issn.2095-0756.2013.04.016
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  • Corresponding author: YANG Xianyu
  • Received Date: 2012-08-26
  • Rev Recd Date: 2012-12-15
  • Publish Date: 2013-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus

doi: 10.11833/j.issn.2095-0756.2013.04.016
    Corresponding author: YANG Xianyu

Abstract: To clone the genes encoding the bioactive polypeptides included in Bufo skin and its secretions, the skin plasmid cDNA library of the adult Japanese toad Bufo japonicus formosus was screened by colony DNA polymerase chain reaction followed by a homologous analysis. Results revealed two full length cDNAs of claudin-4. The transcripts were 1 707 bp and 1 697 bp (GenBank: JX481975 and JX481976,respectively), both containing an open reading frame(ORF) of 630 bp. The same polypeptides consisting of 209 amino acid residues were encoded, although five nucleotides were different in the ORF. The 5 untranslated region (UTR) for the two cDNAs was 50 bp and 41 bp, in that order, while the 3 UTR was 1 027 bp and 1 026 bp,respectively. The homologous analysis indicated that Claudin-4 in B. japonicus formosus had an 88% similarity with Xenopus laevis and 65%-72% similarity with other species including Taeniopygia guttata,Anolis carolinensis,Danio rerio,Ornithorhynchus anatinus,Macaca mulatta,Mus musculus,Homo sapiens. This study will become the basis for further studies on biological functions of Claudin-4 in B. japonicus formosus and the drug development for tumor theraputics.[Ch,5 fig. 21 ref.]

RUAN Jiajia, YUAN Jinqiang, YANG Xianyu. Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus[J]. Journal of Zhejiang A&F University, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016
Citation: RUAN Jiajia, YUAN Jinqiang, YANG Xianyu. Cloning and sequence analysis of claudin-4 cDNA from Bufo japonicus formosus[J]. Journal of Zhejiang A&F University, 2013, 30(4): 561-566. doi: 10.11833/j.issn.2095-0756.2013.04.016

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