Tissue culture and rapid propagation of<em> Caragana sinica</em>

CHEN Jialong; ZHU Jianjun; WU Xiushui; YU Hong′ao

doi:10.11833/j.issn.2095-0756.2013.04.022

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Volume 30 Issue 4

Jul. 2013

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CHEN Jialong, ZHU Jianjun, WU Xiushui, YU Hong′ao. Tissue culture and rapid propagation of Caragana sinica[J]. Journal of Zhejiang A&F University, 2013, 30(4): 611-614. doi: 10.11833/j.issn.2095-0756.2013.04.022

Citation:

CHEN Jialong, ZHU Jianjun, WU Xiushui, YU Hong′ao. Tissue culture and rapid propagation of Caragana sinica[J]. Journal of Zhejiang A&F University, 2013, 30(4): 611-614. doi: 10.11833/j.issn.2095-0756.2013.04.022

Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

1.
Faculty of Landscape Arohitectane，Wenzhou Vocational College of Science & Technology，Wenzhou 325006，Zhejiang，China

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Corresponding author: CHEN Jialong
Received Date: 2012-05-28
Rev Recd Date: 2013-01-07
Publish Date: 2013-08-20

Abstract

Caragana sinica is a edible flower，having good market demand. This aim is to establish a efficient tissue culture system for its commercial propagation. The stem was taken as the explants，this experiment adopted the single factor experimental design，the effects of different plant growth regulator treatments on various processes of tissue culture were studied. Results were as follows：the optimal sterilization method for the explant was dipping in 1.0 gL-1 HgCl2 for about 10 min. The contamination rate（36.7%） and browning rate（16.7%） were better than other treatments. The Murashige and Skoog （MS） culture media containing 2.0 mgL-1 6-benzyladenine （BA） + 0.2 mgL-1 -naphthalene acetic acid （NAA） was suitable for the initial culture，the induction rate was 60.0%（P＜0.05）. For proliferation，the optimal medium was MS supplemented with 0.5 mgL-1 thidiazuron（TDZ） with 5-6 clustered buds being induced，the multiplication rate could reach 4.5（P＜0.05）. When shoots were inoculated with MS supplemented with 0.5 mgL-1 NAA，the rooting rate was up to 86.7%. The results could provide technical guidance for large-scale micropropagation of Caragana sinica. ［Ch，1 fig. 3 tab. 11 ref.］
- horticulture,
- Caragana sinica,
- tissue culture,
- rapid propagation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

1. Faculty of Landscape Arohitectane，Wenzhou Vocational College of Science & Technology，Wenzhou 325006，Zhejiang，China

Corresponding author: CHEN Jialong

Received Date: 2012-05-28
Rev Recd Date: 2013-01-07
Publish Date: 2013-08-20

Key words:

Abstract: Caragana sinica is a edible flower，having good market demand. This aim is to establish a efficient tissue culture system for its commercial propagation. The stem was taken as the explants，this experiment adopted the single factor experimental design，the effects of different plant growth regulator treatments on various processes of tissue culture were studied. Results were as follows：the optimal sterilization method for the explant was dipping in 1.0 gL-1 HgCl2 for about 10 min. The contamination rate（36.7%） and browning rate（16.7%） were better than other treatments. The Murashige and Skoog （MS） culture media containing 2.0 mgL-1 6-benzyladenine （BA） + 0.2 mgL-1 -naphthalene acetic acid （NAA） was suitable for the initial culture，the induction rate was 60.0%（P＜0.05）. For proliferation，the optimal medium was MS supplemented with 0.5 mgL-1 thidiazuron（TDZ） with 5-6 clustered buds being induced，the multiplication rate could reach 4.5（P＜0.05）. When shoots were inoculated with MS supplemented with 0.5 mgL-1 NAA，the rooting rate was up to 86.7%. The results could provide technical guidance for large-scale micropropagation of Caragana sinica. ［Ch，1 fig. 3 tab. 11 ref.］

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Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

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Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

1. Faculty of Landscape Arohitectane，Wenzhou Vocational College of Science & Technology，Wenzhou 325006，Zhejiang，China

Corresponding author: CHEN Jialong

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Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

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Tissue culture and rapid propagation of Caragana sinica

doi: 10.11833/j.issn.2095-0756.2013.04.022

1. Faculty of Landscape Arohitectane，Wenzhou Vocational College of Science & Technology，Wenzhou 325006，Zhejiang，China

Corresponding author: CHEN Jialong

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