Determination of five active components in <em>Eucommia uimoides</em> under simulated experiment of nitrogen deposition by dual-wavelength HPLC

AI Jianguo; DU Jiangli; ZHOU Aicun; JIN Songheng; SONG Xinzhang

doi:10.11833/j.issn.2095-0756.2015.01.009

Volume 32 Issue 1

Jan. 2015

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AI Jianguo, DU Jiangli, ZHOU Aicun, JIN Songheng, SONG Xinzhang. Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC[J]. Journal of Zhejiang A&F University, 2015, 32(1): 60-66. doi: 10.11833/j.issn.2095-0756.2015.01.009

Citation:

AI Jianguo, DU Jiangli, ZHOU Aicun, JIN Songheng, SONG Xinzhang. Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC[J]. Journal of Zhejiang A&F University, 2015, 32(1): 60-66. doi: 10.11833/j.issn.2095-0756.2015.01.009

Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

doi: 10.11833/j.issn.2095-0756.2015.01.009

1.
School of Information Engineering, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;
2.
School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang, China

Received Date: 2014-04-16
Rev Recd Date: 2014-06-24
Publish Date: 2015-02-20

Abstract

A method for determination of pinoresinol diglucoside, aucubin, chlorogenic acid, geniposidic acid and geniposide in Eucommia uimoides under simulated experiment of nitrogen deposition by dual wavelength HPLC was established. Dual-wavelength HPLC was adopted performing on Agilent ZORBAX SB-C18 column （250 mm4.6 mm, 5 m） with a mobile phase of acetonitrile （A）-0.2% phosphoric acid （B） at flow rate of 0.8 mLmin－1. The gradient elution program was as follows: 0－12 min, 10%A; 12－25 min, 13%A; 25－50 min, 30%A; 50－65 min, 60%A. The detection wavelength was set at 203 nm for pinoresinol diglucoside and aucubin, and at 240 nm for chlorogenic acid, geniposidic acid and geniposide. The column temperature was 30 ℃. Results showed that pinoresinol diglucoside, aucubin, chlorogenic acid, geniposidic acid and geniposide all had a good linear relationship with their peak area values respectively. The five ingredients from bark and leaves of Eucommia uimoides had different response to the nitrogen treatments, and changing in the seasons. The method can be used for simultaneous determination of ingredients in Eucommia uimoides, and can be successfully applied to reflect the content changing of the compounds.［Ch, 3 fig. 6 tab. 13 ref.］
- cash forestry,
- Eucornmia ulmoides,
- simulated nitrogen deposition,
- medicinal ingredients,
- dual-wavelength HPLC

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

1. School of Information Engineering, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang, China

Received Date: 2014-04-16

Rev Recd Date: 2014-06-24

Publish Date: 2015-02-20

Key words:

Abstract: A method for determination of pinoresinol diglucoside, aucubin, chlorogenic acid, geniposidic acid and geniposide in Eucommia uimoides under simulated experiment of nitrogen deposition by dual wavelength HPLC was established. Dual-wavelength HPLC was adopted performing on Agilent ZORBAX SB-C18 column （250 mm4.6 mm, 5 m） with a mobile phase of acetonitrile （A）-0.2% phosphoric acid （B） at flow rate of 0.8 mLmin－1. The gradient elution program was as follows: 0－12 min, 10%A; 12－25 min, 13%A; 25－50 min, 30%A; 50－65 min, 60%A. The detection wavelength was set at 203 nm for pinoresinol diglucoside and aucubin, and at 240 nm for chlorogenic acid, geniposidic acid and geniposide. The column temperature was 30 ℃. Results showed that pinoresinol diglucoside, aucubin, chlorogenic acid, geniposidic acid and geniposide all had a good linear relationship with their peak area values respectively. The five ingredients from bark and leaves of Eucommia uimoides had different response to the nitrogen treatments, and changing in the seasons. The method can be used for simultaneous determination of ingredients in Eucommia uimoides, and can be successfully applied to reflect the content changing of the compounds.［Ch, 3 fig. 6 tab. 13 ref.］

Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

doi: 10.11833/j.issn.2095-0756.2015.01.009

Abstract

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Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

doi: 10.11833/j.issn.2095-0756.2015.01.009

1. School of Information Engineering, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang, China

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Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

doi: 10.11833/j.issn.2095-0756.2015.01.009

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通讯作者: 陈斌, bchen63@163.com

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Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

doi: 10.11833/j.issn.2095-0756.2015.01.009

1. School of Information Engineering, Zhejiang A & F University, Lin’an 311300, Zhejiang, China; 2. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang, China

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1. School of Information Engineering, Zhejiang A & F University, Lin’an 311300, Zhejiang, China;

2. School of Forestry and Biotechnology，Zhejiang A & F University，Lin’an 311300，Zhejiang, China