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ZHANG Xian, WANG Jixuan, CHENG Gaochuan, LI Ke, ZHANG Xiaofeng, SUN Mengjiao, CHENG Changyong, SONG Houhui. Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210772
Citation: ZHANG Xian, WANG Jixuan, CHENG Gaochuan, LI Ke, ZHANG Xiaofeng, SUN Mengjiao, CHENG Changyong, SONG Houhui. Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210772

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Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1

doi: 10.11833/j.issn.2095-0756.20210772
  • Received Date: 2021-11-29
  • Accepted Date: 2022-04-01
  • Rev Recd Date: 2022-03-31
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1

doi: 10.11833/j.issn.2095-0756.20210772

Abstract:   Objective  Fungal metabolites, commonly known as mycotoxins, can pollute agricultural products and food of animal origin, among which aflatoxin B1 (AFB1) is the most common, toxic and detrimental. Establishing a rapid, highly sensitive and convenient detection method of AFB1 is of great significance for the protection of human and animal health. The objective of this study is to optimize the immunochromatographic detection method based on the principle of lateral-flow chromatography and competitive mode, so as to realize the rapid qualitative detection and quantitative analysis of AFB1.   Method  A highly sensitive qualitative and quantitative immunochromatographic detection method for AFB1 was established by comparing and analyzing the labeling effects of gold particles of varying sizes, optimizing the material types of each component of immunochromatography, as well as relevant buffer solution and the optimal mass concentration.   Result  The qualitative and quantitative detection limits of the optimized AFB1 immunochromatographic method in samples were 2.5 and 0.5 μg·kg−1, respectively, with high sensitivity and specificity and no cross reaction with other common mycotoxins. Standard addition recovery experiment showed that the method was accurate and stable, and the quantitative detection results of AFB1 natural contamination samples were in good agreement with commercial kit and LC-MS/MS.   Conclusion  The immunochromatographic detection method prepared in this study can be used for rapid qualitative detection and quantitative analysis of AFB1 contamination in samples. It is suitable for grass-roots inspection and quarantine institutions and agricultural product processing enterprises that lack experimental conditions to quickly screen a large number of samples. If the sample test result is suspected to be positive, the instrument method can be used for confirmation, which can reduce the test cost, improve the test efficiency and provide reference for the establishment of immunochromatographic detection methods for other pathogenic microorganisms. [Ch, 9 fig. 2 tab. 26 ref.]

ZHANG Xian, WANG Jixuan, CHENG Gaochuan, LI Ke, ZHANG Xiaofeng, SUN Mengjiao, CHENG Changyong, SONG Houhui. Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210772
Citation: ZHANG Xian, WANG Jixuan, CHENG Gaochuan, LI Ke, ZHANG Xiaofeng, SUN Mengjiao, CHENG Changyong, SONG Houhui. Development of a highly sensitive qualitative and quantitative immunochromatographic method for the detection of aflatoxin B1[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20210772
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