Proteome study of dwarf mutant in <em>Cunninghamia lanceolata</em> by 2D-DIGE

HUANG Hua-hong; TONG Zai-kang; ZHU Yu-qiu; GAO Yan-hui; XU Chang-shou; HE Fu-ji

Volume 23 Issue 3

Feb. 2017

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2006 > 23(3): 265-269

HUANG Hua-hong, TONG Zai-kang, ZHU Yu-qiu, GAO Yan-hui, XU Chang-shou, HE Fu-ji. Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE[J]. Journal of Zhejiang A&F University, 2006, 23(3): 265-269.

Citation:

HUANG Hua-hong, TONG Zai-kang, ZHU Yu-qiu, GAO Yan-hui, XU Chang-shou, HE Fu-ji. Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE[J]. Journal of Zhejiang A&F University, 2006, 23(3): 265-269.

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1.
School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;
2.
.Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

Funds:

浙江省科学技术攻关项目(2004C12022)

Received Date: 2005-09-19
Rev Recd Date: 2006-01-19
Publish Date: 2006-06-20

Abstract

To establish the maps of two-dimensional differential gel electrophoresis , the dwarfing mechanism of the mutant in Cunninghamia lanceolata was analyzed .The leaves from normal and dwarf plants were ground into powder with liquid nitrogen .The total proteins were extracted with the modified TCA-acetone method , labeled with CY2 or CY3 respectively , analyzed by 2D-DIGE .After electrophoresing , the gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder software in each paired normal/dwarf comparison .The results showed that 14 unique proteins the had a 2.2-fold increase and 15 proteins that had a 3.0-fold decrease were found in dwarfed plants .These 29 proteins might be associated with the dwarfing .[ Ch , 3 fig .3 tab .9 ref .]
- forest tree breeding,
- Cunninghamia lanceolata,
- dwarf mutant,
- proteome,
- differential gel electrophoresis (DIGE)

Relative Article

[1]	HE Guiping, XU Jinliang, XU Yongqin, CHEN Yonghui, SHEN Fengqiang, XU Luyu. Growth differences and selection of Chinese fir families in young plantations with different site conditions . Journal of Zhejiang A&F University, 2018, 35(3): 453-458. doi: 10.11833/j.issn.2095-0756.2018.03.008
[2]	LIU Weiyi, DENG Huafeng, RAN Qixiang, HUANG Guosheng, WANG Xuejun. Compatibility of height-diameter curve equations for Cunninghamia lanceolata stands in Hunan Province . Journal of Zhejiang A&F University, 2017, 34(6): 1051-1058. doi: 10.11833/j.issn.2095-0756.2017.06.012
[3]	BI Zhihong, WEI Minjing, LIU Yingying, YANG Chuanping, WEI Zhigang. Genetic parameters of Betula platyphylla provenances due to sample size . Journal of Zhejiang A&F University, 2016, 33(4): 564-570. doi: 10.11833/j.issn.2095-0756.2016.04.003
[4]	CAO Xiaoyu, LI Jiping, FENG Yao, ZHANG Caicai, FANG Xiaona. Stand spatial structure for an uneven-aged Cunninghamia lanceolata ecological forest . Journal of Zhejiang A&F University, 2015, 32(1): 84-91. doi: 10.11833/j.issn.2095-0756.2015.01.012
[5]	PENG Sha-sha, TONG Zai-kang, HUANG Hua-hong, ZHOU Hou-jun, SHI Jian, LIN Er-pei. Cloning and bioimformatic analysis of the cellulose synthase-like protein gene，ClCslD1 from Cunninghamia lanceolata . Journal of Zhejiang A&F University, 2012, 29(1): 1-6. doi: 10.11833/j.issn.2095-0756.2012.01.001
[6]	MA Jin, LIU Zhi-gao, ZHENG Gang. Differential proteomics and its application in plant salt stress . Journal of Zhejiang A&F University, 2011, 28(1): 139-143. doi: 10.11833/j.issn.2095-0756.2011.01.022
[7]	ZHENG Yong-ping, SUN Hong-you, FENG Jian-min, ZHANG Jian-zhang, FENG Jian-guo, WU Long-gao. ^{Genetic improvement on bi-clonal seed orchard of Chinese fir clone Long-15 and Min-33} . Journal of Zhejiang A&F University, 2009, 26(2): 201-208.
[8]	CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis . Journal of Zhejiang A&F University, 2008, 25(1): 22-27.
[9]	LI Jie, XIONG Zhi, ZHANG Cheng-gang. Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR . Journal of Zhejiang A&F University, 2008, 25(1): 16-21.
[10]	LIANG Hong-wen, HUANG Heng-chuan, HUANG Cheng-biao, HUANG Hai-zhong, LIANG Xin, MENG Yue-huan. Wood physical-mechanical properties of Taiwania flousiana compared to Cunninghamia lanceolata for plantation development . Journal of Zhejiang A&F University, 2008, 25(2): 137-142.
[11]	FUShun-hua, DONG Ru-xiang, WULong-gao, FENG Jian-min, LIUWei-hong, HUA Chao-hui, SUNHong-you. Correlations in progeny trials of Cunninghamia lanceolata . Journal of Zhejiang A&F University, 2007, 24(3): 272-278.
[12]	LUO Wen-jian, JIN Guo-qing, XU Gao-fu, FENG Bing-cai, FENG Jian-min, LUO Xiao-hua. Genetic gain and superior families selection from clonal seed orchards of Cupressus funebris . Journal of Zhejiang A&F University, 2006, 23(3): 259-264.
[13]	SUN Hong-you, ZHENG Yong-ping, WENG Chun-mei, LUO Xiao-hua, TU Wu-tai, CAI Ke-xiao. Seed quality character variation and seed genetic parameters of Cunninghamia lanceolata in seed orchard . Journal of Zhejiang A&F University, 2005, 22(1): 61-65.
[14]	HE Zhen-xiang, SHI Ji-sen, YIN Zeng-fang, CHEN Xiao-chou, YU Rong-zhuo. Detection of genetic markers associated with growth traits in Chinese fir . Journal of Zhejiang A&F University, 2000, 17(4): 350-354.
[15]	LIN Tong-long. Chinese fir :genetic variation and combination selection in DBH growth and wood volume weight for filial generation . Journal of Zhejiang A&F University, 2000, 17(2): 142-145.
[16]	Lin Qingfu. Variation in Densities and Shrinkages of Chinese Fir Wood From Plantation. . Journal of Zhejiang A&F University, 1997, 14(2): 169-173.
[17]	Sun Hongyou, Fu Qiuhua, Cheng Yumin, Wang Yuxi, Feng Xiaoyang.. A Primary Study on Genetic Improvement of Chinese Fir Seed Quality . Journal of Zhejiang A&F University, 1997, 14(2): 120-126.
[18]	Lin Kaimin, Yu Xintuo, Qiu Erfa, He Zhiying, Lin Sizu. Nutrient space difference of Chinese Fir Stands with Different Densities. . Journal of Zhejiang A&F University, 1996, 13(3): 243-247.
[19]	Lin Kaimin, Lin Guoqing, Zhang Shenlong, Yu Lixuan.. Differences of soil Fertility between Nautral Broadleaved Forest and Replanted Chinese Fir Forest. . Journal of Zhejiang A&F University, 1995, 12(2): 221-225.
[20]	He Fuji, Yu Xiangyu, Li Ping.. Preliminary Inheritance Analysis to Male Sterility of Chinese Fir. . Journal of Zhejiang A&F University, 1995, 12(2): 219-220.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(1008) PDF downloads(239) Cited by()

Proportional views

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

Funds:

浙江省科学技术攻关项目(2004C12022)

Received Date: 2005-09-19

Rev Recd Date: 2006-01-19

Publish Date: 2006-06-20

Key words:

Abstract: To establish the maps of two-dimensional differential gel electrophoresis , the dwarfing mechanism of the mutant in Cunninghamia lanceolata was analyzed .The leaves from normal and dwarf plants were ground into powder with liquid nitrogen .The total proteins were extracted with the modified TCA-acetone method , labeled with CY2 or CY3 respectively , analyzed by 2D-DIGE .After electrophoresing , the gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder software in each paired normal/dwarf comparison .The results showed that 14 unique proteins the had a 2.2-fold increase and 15 proteins that had a 3.0-fold decrease were found in dwarfed plants .These 29 proteins might be associated with the dwarfing .[ Ch , 3 fig .3 tab .9 ref .]

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

HTML

Catalog

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China; 2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

HTML

Catalog

Export File

Citation

Format

Content

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China