Proteome study of dwarf mutant in <em>Cunninghamia lanceolata</em> by 2D-DIGE

HUANG Hua-hong; TONG Zai-kang; ZHU Yu-qiu; GAO Yan-hui; XU Chang-shou; HE Fu-ji

Volume 23 Issue 3

Feb. 2017

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Article Navigation > Journal of Zhejiang A&F University > 2006 > 23(3): 265-269

HUANG Hua-hong, TONG Zai-kang, ZHU Yu-qiu, GAO Yan-hui, XU Chang-shou, HE Fu-ji. Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE[J]. Journal of Zhejiang A&F University, 2006, 23(3): 265-269.

Citation:

HUANG Hua-hong, TONG Zai-kang, ZHU Yu-qiu, GAO Yan-hui, XU Chang-shou, HE Fu-ji. Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE[J]. Journal of Zhejiang A&F University, 2006, 23(3): 265-269.

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1.
School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;
2.
.Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

Funds:

浙江省科学技术攻关项目(2004C12022)

Received Date: 2005-09-19
Rev Recd Date: 2006-01-19
Publish Date: 2006-06-20

Abstract

To establish the maps of two-dimensional differential gel electrophoresis , the dwarfing mechanism of the mutant in Cunninghamia lanceolata was analyzed .The leaves from normal and dwarf plants were ground into powder with liquid nitrogen .The total proteins were extracted with the modified TCA-acetone method , labeled with CY2 or CY3 respectively , analyzed by 2D-DIGE .After electrophoresing , the gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder software in each paired normal/dwarf comparison .The results showed that 14 unique proteins the had a 2.2-fold increase and 15 proteins that had a 3.0-fold decrease were found in dwarfed plants .These 29 proteins might be associated with the dwarfing .[ Ch , 3 fig .3 tab .9 ref .]
- forest tree breeding,
- Cunninghamia lanceolata,
- dwarf mutant,
- proteome,
- differential gel electrophoresis (DIGE)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

Funds:

浙江省科学技术攻关项目(2004C12022)

Received Date: 2005-09-19

Rev Recd Date: 2006-01-19

Publish Date: 2006-06-20

Key words:

Abstract: To establish the maps of two-dimensional differential gel electrophoresis , the dwarfing mechanism of the mutant in Cunninghamia lanceolata was analyzed .The leaves from normal and dwarf plants were ground into powder with liquid nitrogen .The total proteins were extracted with the modified TCA-acetone method , labeled with CY2 or CY3 respectively , analyzed by 2D-DIGE .After electrophoresing , the gels were respectively imaged by Typhoon 9400 using different emission filter and spot-features were analyzed by DeCyder software in each paired normal/dwarf comparison .The results showed that 14 unique proteins the had a 2.2-fold increase and 15 proteins that had a 3.0-fold decrease were found in dwarfed plants .These 29 proteins might be associated with the dwarfing .[ Ch , 3 fig .3 tab .9 ref .]

Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

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Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

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Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

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通讯作者: 陈斌, bchen63@163.com

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Proteome study of dwarf mutant in Cunninghamia lanceolata by 2D-DIGE

1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China; 2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China

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1. School of Forestry and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;

2. .Maxiao Forest Station of Lin'an City , Lin'an 311323, Zhejiang , China