Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR

LI Jie; XIONG Zhi; ZHANG Cheng-gang

Volume 25 Issue 1

Jun. 2018

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LI Jie, XIONG Zhi, ZHANG Cheng-gang. Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR[J]. Journal of Zhejiang A&F University, 2008, 25(1): 16-21.

Citation:

LI Jie, XIONG Zhi, ZHANG Cheng-gang. Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR[J]. Journal of Zhejiang A&F University, 2008, 25(1): 16-21.

Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR

1.
Resources Faculty, Southwest Forestry College, Kunming 650224, Yunnan, China;
2.
Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110015, Liaoning, China

Received Date: 2007-01-05
Rev Recd Date: 2007-04-18
Publish Date: 2008-02-10

Abstract

Alnus nepalensis is the oldest species of Betulaceae, mainly distributed in Yunnan Province, which can fix nitrogen. The objective is to study its genetic diversity and assess its resources. In Yunnan Province 58 naturally occurring samples of A.nepalensis were collected from Laifeng, Gaoligong, Jizu, Cang, and Wuliang Mountains. The genomic DNA of A.nepalensis was as extracted by the cetyltrimethylammonium bromide(CTAB) method,and their DNA polymorphism was analyzed by random amplification of polymorphic DNA(RAPD)-polymerase chain reaction(PCR) with 3 optimum primers. A total of 85 DNA bands were amplified, and a DNA molecular UPGMA(unweighted pair group method using arithmetic averages) dendrogram was established. Also, for the samples,Nei's gene diversity and Shannon's information index were computed by POPGENE software Numerical Taxonomy SYStem(NTSYS) software. Results indicated that 64 of the 85 DNA bands(75.3%) were polymorphic, and with the dendrogram A.nepalensis samples were placed into 6 groups with a standard similarity coefficient of 0.68. A.nepalensis possessed abundant genetic diversity with the highest diversity found in the Jizu Mountains. In the Wuliang Mountains genetic diversity of A.nepalensis was richer along the southern slope than the northern. Also, a close relationship between the genetic structure of A.nepalensis and the environment was shown with genetic diversity.
- forest breeding,
- Alnus nepalensis,
- genetic diversity,
- random amplification of polymorphic DNA(RAPD)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR

1. Resources Faculty, Southwest Forestry College, Kunming 650224, Yunnan, China;

2. Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110015, Liaoning, China

Received Date: 2007-01-05

Rev Recd Date: 2007-04-18

Publish Date: 2008-02-10

Key words:

forest breeding /
Alnus nepalensis /
genetic diversity /
random amplification of polymorphic DNA(RAPD)

Abstract: Alnus nepalensis is the oldest species of Betulaceae, mainly distributed in Yunnan Province, which can fix nitrogen. The objective is to study its genetic diversity and assess its resources. In Yunnan Province 58 naturally occurring samples of A.nepalensis were collected from Laifeng, Gaoligong, Jizu, Cang, and Wuliang Mountains. The genomic DNA of A.nepalensis was as extracted by the cetyltrimethylammonium bromide(CTAB) method,and their DNA polymorphism was analyzed by random amplification of polymorphic DNA(RAPD)-polymerase chain reaction(PCR) with 3 optimum primers. A total of 85 DNA bands were amplified, and a DNA molecular UPGMA(unweighted pair group method using arithmetic averages) dendrogram was established. Also, for the samples,Nei's gene diversity and Shannon's information index were computed by POPGENE software Numerical Taxonomy SYStem(NTSYS) software. Results indicated that 64 of the 85 DNA bands(75.3%) were polymorphic, and with the dendrogram A.nepalensis samples were placed into 6 groups with a standard similarity coefficient of 0.68. A.nepalensis possessed abundant genetic diversity with the highest diversity found in the Jizu Mountains. In the Wuliang Mountains genetic diversity of A.nepalensis was richer along the southern slope than the northern. Also, a close relationship between the genetic structure of A.nepalensis and the environment was shown with genetic diversity.

Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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