Cloning and analysis of the APETALA1（AP1） homolog gene from <EM>Carya cathayensis</EM>

WANG Zheng-jia; HUANG You-jun; XIA Guo-hua; ZHENG Bing-song; JIN Song-heng; HUANG Jian-qin

Volume 25 Issue 4

Aug. 2008

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Article Navigation > Journal of Zhejiang A&F University > 2008 > 25(4): 427-430

WANG Zheng-jia, HUANG You-jun, XIA Guo-hua, ZHENG Bing-song, JIN Song-heng, HUANG Jian-qin. Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 427-430.

Citation:

WANG Zheng-jia, HUANG You-jun, XIA Guo-hua, ZHENG Bing-song, JIN Song-heng, HUANG Jian-qin. Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis[J]. Journal of Zhejiang A&F University, 2008, 25(4): 427-430.

Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

1.
The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

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Corresponding author: HUANG Jian-qin
Publish Date: 2008-08-20

Abstract

From Carya cathayensis，23 base pair （bp） primers designed according to a conserved sequence of the APETALA1 （AP1） gene，which has an important function in floral development，were used to amplify a 486 bp fragment by polymerase chain reaction（PCR）. The fragment was cloned into a pMD18-T vector and then sequenced. Next，the deduced amino acid sequence of the fragment was blasted with AP1 homologous genes of other 17 plants. Results of the 486 bp fragment cloned into a pMD18-T vector produced a fragment of the AP1 homologous gene：the CcAP1 gene. Also，the sequence analysis indicated two introns of 86 bp and 291 bp in the fragment with the exons encoding 36 amino acids. Afterward，when the CcAP1 gene was blasted with AP1 homologous genes，homology reached 69%－88%. This result suggested that the CcAP1 gene，registered in GenBank with the accession number EU155118，might have the same function of floral development as other AP1 homologous genes. ［Ch，3 fig. 1 tab. 11 ref.］
- forest tree breeding,
- Carya cathayensis,
- APETALA1 gene,
- cloning,
- sequence analysis

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

Publish Date: 2008-08-20

Key words:

Abstract: From Carya cathayensis，23 base pair （bp） primers designed according to a conserved sequence of the APETALA1 （AP1） gene，which has an important function in floral development，were used to amplify a 486 bp fragment by polymerase chain reaction（PCR）. The fragment was cloned into a pMD18-T vector and then sequenced. Next，the deduced amino acid sequence of the fragment was blasted with AP1 homologous genes of other 17 plants. Results of the 486 bp fragment cloned into a pMD18-T vector produced a fragment of the AP1 homologous gene：the CcAP1 gene. Also，the sequence analysis indicated two introns of 86 bp and 291 bp in the fragment with the exons encoding 36 amino acids. Afterward，when the CcAP1 gene was blasted with AP1 homologous genes，homology reached 69%－88%. This result suggested that the CcAP1 gene，registered in GenBank with the accession number EU155118，might have the same function of floral development as other AP1 homologous genes. ［Ch，3 fig. 1 tab. 11 ref.］

Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

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Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

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Cloning and analysis of the APETALA1（AP1） homolog gene from Carya cathayensis

1. The Key Laboratory for Modern Silvicultural Technology of Zhejiang Province，School of Forestry and Biotechnology，Zhejiang Forestry College，Lin’an 311300，Zhejiang，China

Corresponding author: HUANG Jian-qin

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