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竹茎秆快速生长期淀粉分解相关酶基因表达的分析

栗青丽 王灵杰 高培军 韦赛君 吕嘉欣 高岩 张汝民

栗青丽, 王灵杰, 高培军, 韦赛君, 吕嘉欣, 高岩, 张汝民. 竹茎秆快速生长期淀粉分解相关酶基因表达的分析[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20190661
引用本文: 栗青丽, 王灵杰, 高培军, 韦赛君, 吕嘉欣, 高岩, 张汝民. 竹茎秆快速生长期淀粉分解相关酶基因表达的分析[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20190661
LI Qingli, WANG Lingjie, GAO Peijun, WEI Saijun, LÜ Jiaxin, GAO Yan, ZHANG Rumin. Gene expression of starch decomposing enzymes in Phyllostachys edulis stems during the rapid growth period[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20190661
Citation: LI Qingli, WANG Lingjie, GAO Peijun, WEI Saijun, LÜ Jiaxin, GAO Yan, ZHANG Rumin. Gene expression of starch decomposing enzymes in Phyllostachys edulis stems during the rapid growth period[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20190661

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竹茎秆快速生长期淀粉分解相关酶基因表达的分析

doi: 10.11833/j.issn.2095-0756.20190661
基金项目: 国家自然科学基金资助项目(31570686)
详细信息
    作者简介: 栗青丽,从事植物生理生态学研究。E-mail: 1403123171@qq.com
    通信作者: 高培军,副教授,博士,从事竹林培育与利用研究。E-mail: 316919866@qq.com
  • 中图分类号: S722.3

Gene expression of starch decomposing enzymes in Phyllostachys edulis stems during the rapid growth period

  • 摘要:   目的  揭示毛竹Phyllostachys edulis茎秆内α-淀粉酶(AMY)和β-淀粉酶(BAM)以及相关基因表达对毛竹快速生长的调控机制。  方法  以毛竹笋竹为试材,测定茎秆不同时间(10:00、14:00、18:00、22:00、2:00、6:00)和不同节间(第7、10、13、16节)内非结构性碳水化合物(NSC)质量分数、α-淀粉酶活性和β-淀粉酶活性以及PeAMYPeBAM基因表达。  结果  毛竹茎秆NSC质量分数白天较高,18:00之后逐渐降低,第7节和第10节葡萄糖质量分数2:00分别比18:00降低了25.4%和27.2%(P<0.01),果糖分别降低了50.0%和34.1%(P<0.01),蔗糖分别降低了49.8%和27.4%(P<0.01),淀粉6:00分别比18:00降低了27.3%和23.2%(P<0.01);BAM活性和基因表达在白天较稳定,18:00之后极显著升高,黎明前逐渐下降,第7、10和13节22:00活性分别比18:00高90.5%,76.7%和50.5%(P<0.01),PeBAM基因表达2:00分别比18:00高1.8、1.8和1.7倍(P<0.01)。  结论  毛竹茎秆快速生长期白天生长慢,夜间生长快,茎秆发育和成熟是从下往上顺次推进的。毛竹茎秆快速生长与PeBAM基因的表达密切相关,BAM在毛竹茎秆淀粉降解中可能起主要作用。图4表1参38
  • 图  1  毛竹茎秆不同节间碳水化合物质量分数的日变化

    Figure  1  Diurnal variation of carbohydrate contents in different internodes for stems of Ph. edulis

    图  2  毛竹茎秆不同节间淀粉酶活性的日变化

    Figure  2  Diurnal variation of amylase activity in different internodes for stems of Ph. edulis

    图  3  毛竹茎秆不同节间麦芽糖酶活性的日变化

    Figure  3  Diurnal variation of maltase activity in different internode for stems of Ph. edulis

    图  4  毛竹茎秆不同节间淀粉酶基因表达的日变化

    Figure  4  Diurnal variation of amylase gene expression in different internode for stems of Ph. edulis

    表  1  引物信息

    Table  1.   Primer information

    基因名称序列用途
    PeAMY-FCATTTCTTCGACTGGGGCCT基因全长扩增
    PeAMY-RGATCACCTTGCCGTCGATCT
    PeAMY-FTTTTTGCGGTGGGCGAATAC荧光定量PCR
    PeAMY-RAAATGCGGCACACAACACTC
    PeBAM-FCGGCAGGATTCTACAACCCT基因全长扩增
    PeBAM-RCCTTCAATGTTCTGGGTAGCC
    PeBAM-FCAGCGAAGCCGAGGAATGAT荧光定量PCR
    PeBAM-RAATGGGGGTAGCTGACGGTA
    PeNTB-FTCTTGTTTGACACCGAAGAGGAG内参基因  
    PeNTB-RAATAGCTGTCCCTGGAGGAGTT
    下载: 导出CSV
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    http://zlxb.zafu.edu.cn/article/zjnldxxb/2020/5/1

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出版历程
  • 收稿日期:  2019-11-05
  • 修回日期:  2020-05-24

竹茎秆快速生长期淀粉分解相关酶基因表达的分析

doi: 10.11833/j.issn.2095-0756.20190661
    基金项目:  国家自然科学基金资助项目(31570686)
    作者简介:

    栗青丽,从事植物生理生态学研究。E-mail: 1403123171@qq.com

    通信作者: 高培军,副教授,博士,从事竹林培育与利用研究。E-mail: 316919866@qq.com
  • 中图分类号: S722.3

摘要:   目的  揭示毛竹Phyllostachys edulis茎秆内α-淀粉酶(AMY)和β-淀粉酶(BAM)以及相关基因表达对毛竹快速生长的调控机制。  方法  以毛竹笋竹为试材,测定茎秆不同时间(10:00、14:00、18:00、22:00、2:00、6:00)和不同节间(第7、10、13、16节)内非结构性碳水化合物(NSC)质量分数、α-淀粉酶活性和β-淀粉酶活性以及PeAMYPeBAM基因表达。  结果  毛竹茎秆NSC质量分数白天较高,18:00之后逐渐降低,第7节和第10节葡萄糖质量分数2:00分别比18:00降低了25.4%和27.2%(P<0.01),果糖分别降低了50.0%和34.1%(P<0.01),蔗糖分别降低了49.8%和27.4%(P<0.01),淀粉6:00分别比18:00降低了27.3%和23.2%(P<0.01);BAM活性和基因表达在白天较稳定,18:00之后极显著升高,黎明前逐渐下降,第7、10和13节22:00活性分别比18:00高90.5%,76.7%和50.5%(P<0.01),PeBAM基因表达2:00分别比18:00高1.8、1.8和1.7倍(P<0.01)。  结论  毛竹茎秆快速生长期白天生长慢,夜间生长快,茎秆发育和成熟是从下往上顺次推进的。毛竹茎秆快速生长与PeBAM基因的表达密切相关,BAM在毛竹茎秆淀粉降解中可能起主要作用。图4表1参38

English Abstract

栗青丽, 王灵杰, 高培军, 韦赛君, 吕嘉欣, 高岩, 张汝民. 竹茎秆快速生长期淀粉分解相关酶基因表达的分析[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20190661
引用本文: 栗青丽, 王灵杰, 高培军, 韦赛君, 吕嘉欣, 高岩, 张汝民. 竹茎秆快速生长期淀粉分解相关酶基因表达的分析[J]. 浙江农林大学学报. doi: 10.11833/j.issn.2095-0756.20190661
LI Qingli, WANG Lingjie, GAO Peijun, WEI Saijun, LÜ Jiaxin, GAO Yan, ZHANG Rumin. Gene expression of starch decomposing enzymes in Phyllostachys edulis stems during the rapid growth period[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20190661
Citation: LI Qingli, WANG Lingjie, GAO Peijun, WEI Saijun, LÜ Jiaxin, GAO Yan, ZHANG Rumin. Gene expression of starch decomposing enzymes in Phyllostachys edulis stems during the rapid growth period[J]. Journal of Zhejiang A&F University. doi: 10.11833/j.issn.2095-0756.20190661

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