Keywords: 
• botany /
• Phyllostachys edulis /
• pyruvate, orthophosphate dikinase regulatory proteins /
• gene cloning /
• prokaryotic expression /
• protein purification

Abstract: Pyruvate orthophosphate dikinase regulatory protein （PPDK-RP） is a key protein in C4 photosynthesis and can modified enzyme activity of PPDK. For Phyllostachys edulis, an important economic bamboo species, cloning PPDK-RP gene and studying its functions had vital theoretical value and application for bamboo photosynthesis research. Firstly PeRP1 was successfully cloned by reverse transcription polymerase chain reaction （RT-PCR）, and afterward a multiple sequence alignment and phylogenetic analysis was conducted. Then for further study the crystal structure of the PeRP protein, the recombinant expression pe-SUMO vectors of PeRP were constructed and the protein was expressed in E. coli and purified by nickle beads column and size column. Results showed that the PeRP gene contained a 1.275 kb open reading frame （ORF） coding a 425 amino acid polypeptide, which contained the typical domain of UDF299 and was predicted as a number of kinase-PPPase superfamily. The multiple sequence alignment and phylogenetic analysis of PeRP revealed that Phyllostachys edulis was a typical C3 monocotyledon. Expressed soluble PeRP1 protein had three forms--polymer, dimmers, and monomers in water soultion. These provided a foundation for the structure and function of RP.［Ch, 7 fig. 15 ref.］