Plant growth regulator treatments for a tissue culture of Euodia rutaecarpa

Key words: 
• botany /
• Euodia rutaecarpa /
• plant growth regulators /
• tissue culture /
• induction

Abstract: To determine the effects of different plant growth regulator treatments，including 6-benzylaminopurine （6-BA），1-naphthlcetic acid （NAA），2，4-dichlorophenoxy acetic acid （2，4-D），and N-phenyl-N-1，2， 3-thidiazol-5-yl-urea（TDZ），on various processes of tissue culture in female plants of Euodia rutaecarpa （Juss.） Benth，leaves of regenerated shoots were used. Results showed that 1.0 mgL-1 6-BA alone or with 0.1 mgL-1 NAA induced both callus and adventitious buds. The other plant growth regulator treatments induced the formation of callus，but failed to induce adventitious buds. NAA by itself did not induce adventitious buds，but it did promote the effects of 6-BA. As the concentration of 6-BA increased，the rate of bud differentiation first increased and then declined，a similar trend happened with 6-BA on morphogenesis of adventitious buds. With an optimal concentration，6-BA promoted proliferation，whereas a high concentration led to vitrification. Also，0 - 0.1 mgL-1 NAA promoted rooting and roots appeared faster and stouter as NAA increased. Moreover，the survival rate after transplanting increased. However，0.5 mgL-1 NAA inhibited the growth of lateral roots and the transplanting procedure. After transplanting in the field for 1.5 years，the regenerated plants bloomed.［Ch，1 fig. 4 tab. 10 ref.]