Construction of a large genomic DNA fragments， BIBAC library for <em>Phyllostachys pubescens</em>

GUAN Yu; YANG Yang; ZHANG Zhi-jun; LUO Shu-ping; TANG Ding-qin

doi:10.11833/j.issn.2095-0756.2011.04.001

Volume 28 Issue 4

Aug. 2011

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Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(4): 527-532

GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

Citation:

GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

More Information

Corresponding author: ZHANG Zhi-jun
Publish Date: 2011-08-20

Abstract

One plant-transformation-competent binary bacterial artificial chromosome（BIBAC） library was constructed which represents the first large genomic DNA fragment library generated for Phyllostachys pubescens. High-quality，genomic DNA extracted from young leaves of Phyllostachys pubescens was gradiently enzyme-digested with a gradient using BamH I. Desirable DNA fragments were isolated by pulsed field gel electrophoresis，ligated to the dephosphorylatedion carrier pCLD04541 with a mass ratio of 3∶1，and then transformed to Escherichia coli DH10B competent cells. This was followed by blue-white screening with establishment of a binary bacterial artificial chromosome（BIBAC） genome library. Results showed a high recombination positive colony from which a BIBAC genome library，consisting of 104 clones with an average insert fragment size of about 105 kb after detection with a pulsed field gel electrophoresis, was constructed. The BIBAC library was 5 times larger than the Phyllostachys pubescens genome. The construction of this BIBAC genome library laid a good foundation for related genome research. ［Ch，8 fig. 18 ref.］
- molecular biology,
- Phyllostachys pubescens,
- binary bacterial artificial chromosome （BIBAC）,
- genome library

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

Publish Date: 2011-08-20

Key words:

molecular biology /
Phyllostachys pubescens /
binary bacterial artificial chromosome （BIBAC） /
genome library

Abstract: One plant-transformation-competent binary bacterial artificial chromosome（BIBAC） library was constructed which represents the first large genomic DNA fragment library generated for Phyllostachys pubescens. High-quality，genomic DNA extracted from young leaves of Phyllostachys pubescens was gradiently enzyme-digested with a gradient using BamH I. Desirable DNA fragments were isolated by pulsed field gel electrophoresis，ligated to the dephosphorylatedion carrier pCLD04541 with a mass ratio of 3∶1，and then transformed to Escherichia coli DH10B competent cells. This was followed by blue-white screening with establishment of a binary bacterial artificial chromosome（BIBAC） genome library. Results showed a high recombination positive colony from which a BIBAC genome library，consisting of 104 clones with an average insert fragment size of about 105 kb after detection with a pulsed field gel electrophoresis, was constructed. The BIBAC library was 5 times larger than the Phyllostachys pubescens genome. The construction of this BIBAC genome library laid a good foundation for related genome research. ［Ch，8 fig. 18 ref.］

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

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Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

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1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China