Volume 28 Issue 4
Aug.  2011
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GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001
Citation: GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001
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  • Corresponding author: ZHANG Zhi-jun
  • Publish Date: 2011-08-20
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001
    Corresponding author: ZHANG Zhi-jun

Abstract: One plant-transformation-competent binary bacterial artificial chromosome(BIBAC) library was constructed which represents the first large genomic DNA fragment library generated for Phyllostachys pubescens. High-quality,genomic DNA extracted from young leaves of Phyllostachys pubescens was gradiently enzyme-digested with a gradient using BamH I. Desirable DNA fragments were isolated by pulsed field gel electrophoresis,ligated to the dephosphorylatedion carrier pCLD04541 with a mass ratio of 3∶1,and then transformed to Escherichia coli DH10B competent cells. This was followed by blue-white screening with establishment of a binary bacterial artificial chromosome(BIBAC) genome library. Results showed a high recombination positive colony from which a BIBAC genome library,consisting of 104 clones with an average insert fragment size of about 105 kb after detection with a pulsed field gel electrophoresis, was constructed. The BIBAC library was 5 times larger than the Phyllostachys pubescens genome. The construction of this BIBAC genome library laid a good foundation for related genome research. [Ch,8 fig. 18 ref.]

GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001
Citation: GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments, BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

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