Construction of a large genomic DNA fragments， BIBAC library for <em>Phyllostachys pubescens</em>

GUAN Yu; YANG Yang; ZHANG Zhi-jun; LUO Shu-ping; TANG Ding-qin

doi:10.11833/j.issn.2095-0756.2011.04.001

Volume 28 Issue 4

Aug. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2011 > 28(4): 527-532

GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

Citation:

GUAN Yu, YANG Yang, ZHANG Zhi-jun, LUO Shu-ping, TANG Ding-qin. Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens[J]. Journal of Zhejiang A&F University, 2011, 28(4): 527-532. doi: 10.11833/j.issn.2095-0756.2011.04.001

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;
2.
College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

More Information

Corresponding author: ZHANG Zhi-jun
Publish Date: 2011-08-20

Abstract

One plant-transformation-competent binary bacterial artificial chromosome（BIBAC） library was constructed which represents the first large genomic DNA fragment library generated for Phyllostachys pubescens. High-quality，genomic DNA extracted from young leaves of Phyllostachys pubescens was gradiently enzyme-digested with a gradient using BamH I. Desirable DNA fragments were isolated by pulsed field gel electrophoresis，ligated to the dephosphorylatedion carrier pCLD04541 with a mass ratio of 3∶1，and then transformed to Escherichia coli DH10B competent cells. This was followed by blue-white screening with establishment of a binary bacterial artificial chromosome（BIBAC） genome library. Results showed a high recombination positive colony from which a BIBAC genome library，consisting of 104 clones with an average insert fragment size of about 105 kb after detection with a pulsed field gel electrophoresis, was constructed. The BIBAC library was 5 times larger than the Phyllostachys pubescens genome. The construction of this BIBAC genome library laid a good foundation for related genome research. ［Ch，8 fig. 18 ref.］
- molecular biology,
- Phyllostachys pubescens,
- binary bacterial artificial chromosome （BIBAC）,
- genome library

Relative Article

[1]	WANG Shaoliang, ZHANG Wenyu, GAO Zhimin, ZHOU Mingbing, YANG Kebin, SONG Xinzhang. Identification and expression pattern of phosphorus transporter Ⅰ family genes of Phyllostachys edulis . Journal of Zhejiang A&F University, 2022, 39(3): 486-494. doi: 10.11833/j.issn.2095-0756.20210471
[2]	WANG Lingjie, LI Qingli, GAO Peijun, WEI Saijun, LÜ Jiaxin, GAO Yan, ZHANG Rumin. Activities of key enzymes involved in photosynthesis and expression patterns of corresponding genes during rapid growth of Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(1): 84-92. doi: 10.11833/j.issn.2095-0756.20200277
[3]	LOU Yongfeng, GAO Zhimin. Cloning and functional analysis of early light induced protein genes of Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(1): 93-102. doi: 10.11833/j.issn.2095-0756.20200237
[4]	CHEN Yaxin, ZHOU Mingbing. Genome-wide characteristics and evolution analysis of long terminal repeat retrotransposons in Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(3): 455-463. doi: 10.11833/j.issn.2095-0756.20200458
[5]	RUAN Shiyu, ZHANG Zhijun, CHEN Jialu, MA Ruifang, ZHU Fengxiao, LIU Xiaoyu. Genome identification and expression analysis of GRF gene family in Phyllostachys edulis . Journal of Zhejiang A&F University, 2021, 38(4): 792-801. doi: 10.11833/j.issn.2095-0756.20200544
[6]	CHEN Rongfen, HUANG Jianqin, CHEN Rong, XU Chuanmei. On the application of flow cytometry in the genomic size determination of bamboo plants . Journal of Zhejiang A&F University, 2021, 38(1): 103-111. doi: 10.11833/j.issn.2095-0756.20200212
[7]	BU Keli, FU Lucheng, WANG Lingjie, LI Qingli, WANG Keyang, MA Yuandan, GAO Yan, ZHANG Rumin. Analysis of PeATG1/PeATG4 gene expression in Phyllostachys edulis during rapid growth . Journal of Zhejiang A&F University, 2020, 37(1): 43-50. doi: 10.11833/j.issn.2095-0756.2020.01.006
[8]	LIU Hua, XUE Jinman, XU Qianyu, YI Xin, WANG Weiyan, LIU Hongbo. Spatio-temporal expression of fatty acid desaturase genes AhFAD2A and AhFAD2B in peanuts . Journal of Zhejiang A&F University, 2019, 36(1): 14-20. doi: 10.11833/j.issn.2095-0756.2019.01.003
[9]	ZHAO Lihua, HUANG Chengpeng, WANG Yueyue, HUANG Zhangting. Stability of Phyllostachys edulis phytolith by scanning electron microscopy . Journal of Zhejiang A&F University, 2018, 35(6): 1177-1181. doi: 10.11833/j.issn.2095-0756.2018.06.023
[10]	LI Xiuyun, CHEN Xiaopei, XU Yingwu, CAO Youzhi. Temporo-spatial expressions and prediction of cellulose synthase gene functions with growth of Phyllostachys edulis . Journal of Zhejiang A&F University, 2017, 34(4): 565-573. doi: 10.11833/j.issn.2095-0756.2017.04.001
[11]	HE Shaohai, YAO Shengcun, FENG Yukai, WANG Ke, LI Feifei. Transformation of the bar gene into embryogenic calli of rice via Agrobacterium tumefaciens . Journal of Zhejiang A&F University, 2017, 34(1): 129-136. doi: 10.11833/j.issn.2095-0756.2017.01.018
[12]	HUANG Xiaoyu, XU Zaien, GUO Xiaoqin. Synonymous codon bias of Phyllostachys edulis . Journal of Zhejiang A&F University, 2017, 34(1): 120-128. doi: 10.11833/j.issn.2095-0756.2017.01.017
[13]	CHENG Zhanchao, HOU Dan, MA Yanjun, GAO Jian. Bioinformatic analysis and differential expression of auxin response factor (ARF) gene in Phyllostachys edulis . Journal of Zhejiang A&F University, 2017, 34(4): 574-580. doi: 10.11833/j.issn.2095-0756.2017.04.002
[14]	LIU Jianjian, LIU Junli, JI Minjie, CHEN Jiadong, YANG Xiaofeng, CHEN Aiqun. Identification and expression analysis of tomato plasma membrane H⁺-ATPase family genes . Journal of Zhejiang A&F University, 2016, 33(5): 734-741. doi: 10.11833/j.issn.2095-0756.2016.05.002
[15]	XU Bingqing, AN Miaomiao, JIANG Keyi, XU Lili, YANG Haiyun, ZHOU Mingbing. Cloning and functional analysis of the psbD gene in Pseudosasa japonica f. akebonosuji . Journal of Zhejiang A&F University, 2015, 32(4): 557-565. doi: 10.11833/j.issn.2095-0756.2015.04.010
[16]	LI Beilei, SONG Zhaoliang, JIANG Peikun, ZHOU Guomo, LI Zimin. Phytolith distribution and carbon sequestration in China with Phyllostachys edulis . Journal of Zhejiang A&F University, 2014, 31(4): 547-553. doi: 10.11833/j.issn.2095-0756.2014.04.009
[17]	WANG Yue-yuan, LIU Xiang-min, ZHOU Ming-bing, TANG Ding-qin. 小佛肚竹生氰糖苷合成关键酶CYP79家族同源基因的克隆和鉴定 . Journal of Zhejiang A&F University, 2012, 29(4): 510-515. doi: 10.11833/j.issn.2095-0756.2012.04.005
[18]	XU Xiao-yan, TIAN Min, WANG Cai-xia, LONG Ming-hua. Cloning and expression analysis of a flowering-related gene，OAP 3，in Oncidium . Journal of Zhejiang A&F University, 2011, 28(6): 900-906. doi: 10.11833/j.issn.2095-0756.2011.06.010
[19]	KONG Hong, CHENG Fang-yun. Cytological and molecular biological evidence for taxonomic revision of Paeonia delavayi . Journal of Zhejiang A&F University, 2010, 27(4): 601-605. doi: 10.11833/j.issn.2095-0756.2010.04.021
[20]	XU Qiu-fang, JIANG Pei-kun. Effects of fertilizing on biological properties of root region soil under Phyllostachys pubescens forest . Journal of Zhejiang A&F University, 2000, 17(4): 364-368.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(4782) PDF downloads(1810) Cited by()

Proportional views

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

Publish Date: 2011-08-20

Key words:

molecular biology /
Phyllostachys pubescens /
binary bacterial artificial chromosome （BIBAC） /
genome library

Abstract: One plant-transformation-competent binary bacterial artificial chromosome（BIBAC） library was constructed which represents the first large genomic DNA fragment library generated for Phyllostachys pubescens. High-quality，genomic DNA extracted from young leaves of Phyllostachys pubescens was gradiently enzyme-digested with a gradient using BamH I. Desirable DNA fragments were isolated by pulsed field gel electrophoresis，ligated to the dephosphorylatedion carrier pCLD04541 with a mass ratio of 3∶1，and then transformed to Escherichia coli DH10B competent cells. This was followed by blue-white screening with establishment of a binary bacterial artificial chromosome（BIBAC） genome library. Results showed a high recombination positive colony from which a BIBAC genome library，consisting of 104 clones with an average insert fragment size of about 105 kb after detection with a pulsed field gel electrophoresis, was constructed. The BIBAC library was 5 times larger than the Phyllostachys pubescens genome. The construction of this BIBAC genome library laid a good foundation for related genome research. ［Ch，8 fig. 18 ref.］

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

HTML

Catalog

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Construction of a large genomic DNA fragments， BIBAC library for Phyllostachys pubescens

doi: 10.11833/j.issn.2095-0756.2011.04.001

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China; 2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China

Corresponding author: ZHANG Zhi-jun

HTML

Catalog

Export File

Citation

Format

Content

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;

2. College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，Xinjiang，China