Diversity analysis with RAPD for natural and breeding populations of <em>Larix gmelinii</em>

ZHANG Zhen; ZHANG Han-guo; ZHANG Lei; ZHU Hang-yong; LI Xue-feng

doi:10.11833/j.issn.2095-0756.2012.01.022

Volume 29 Issue 1

Feb. 2012

Turn off MathJax

Article Contents

Article Navigation > Journal of Zhejiang A&F University > 2012 > 29(1): 130-136

ZHANG Zhen, ZHANG Han-guo, ZHANG Lei, ZHU Hang-yong, LI Xue-feng. Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii[J]. Journal of Zhejiang A&F University, 2012, 29(1): 130-136. doi: 10.11833/j.issn.2095-0756.2012.01.022

Citation:

ZHANG Zhen, ZHANG Han-guo, ZHANG Lei, ZHU Hang-yong, LI Xue-feng. Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii[J]. Journal of Zhejiang A&F University, 2012, 29(1): 130-136. doi: 10.11833/j.issn.2095-0756.2012.01.022

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

doi: 10.11833/j.issn.2095-0756.2012.01.022

1.
State Key Laboratory of Forest Genetics and Tree Breeding，Northeast Forestry University，Harbin 150040，Heilongjiang，China

More Information

Corresponding author: ZHANG Han-guo
Received Date: 2011-03-28
Rev Recd Date: 2011-05-23
Publish Date: 2012-02-20

Abstract

Random amplified polymorphic DNA （RAPD） markers associated with phenotypic characteristics were used to analyze the genetic variation of 270 individual samples from six natural populations of Larix gmelinii （Wuyiling，Ganhe，Kuduer，Nenjiang，Moerdaoga，and Aershan）. From 450 primers，23 clear RAPD primers were selected as their amplified bands with high polymorphism. The analysis included the gene diversity index，Shannons diversity index （SI），Neis gene diversity index，a cluster analysis，and a correlation analysis. Results showed 182 loci among a total of 186 were polymorphic or a percentage of polymorphism （PPB） of 97.9% having a range of 87.10%-94.09%. The gene diversity index was 0.374 3，and the genetic diversity within populations was 0.051 7 indicating that genetic variation was mainly within the population （86.19%）. Mean SI was 0.482 1，and the mean for Neis index was 0.322 6. The cluster analysis revealed six populations divided into three groups （the Xiaoxingan Mountain Range，northwest of the Daxingan Mountain Range，and south of the Daxingan Mountain Range） with a genetic distance of 0.09. Genetic distance was significantly and positively correlated with geographical distance（P＜0.05，r=0.768）. Growth traits of the four L. gmelinii populations were 10.25%-20.78% for height，14.93%-28.37% for diameter，and 35.03%-74.36% for volume coefficients of variation. Compared with the genetic variation of breeding populations，in natural populations PPB was 5.32% greater，mean of effective alleles numbered 1.13% greater，Neis index was 4.94% greater，SI was 5.68% greater，and the populations total genetic variation 8.78% greater.［Ch，3 fig. 6 tab. 10 ref.］
- forest tree breeding,
- Larix gmelinii,
- natural population,
- breeding population,
- RAPD,
- growth traits,
- genetic diversity

Relative Article

[1]	DENG Lili, LIU Qinghua, ZHOU Zhichun, GAO Kai, LUO Dinghui. Genetic diversity analysis and core collection of pinewood nematodiasis-resistant Pinus massoniana germplasm resources . Journal of Zhejiang A&F University, 2024, 41(1): 67-78. doi: 10.11833/j.issn.2095-0756.20230333
[2]	LI Yaping, DAI Huiming, JIANG Wu, CHEN Jiadong, DUAN Xiaojing, TAO Zhengming. Genetic diversity of Polygonatum spp. from different production areas based on SRAP markers . Journal of Zhejiang A&F University, 2023, 40(3): 658-664. doi: 10.11833/j.issn.2095-0756.20220436
[3]	WANG Jie, ZHU Xipeng, WANG Tengfei, ZHU Jianjun, LI Wenjun, XING Bingcong, ZHENG Ying. Genetic diversity analysis of Bletilla striata germplasm by ISSR and SRAP markers . Journal of Zhejiang A&F University, 2023, 40(2): 321-329. doi: 10.11833/j.issn.2095-0756.20210690
[4]	ZHENG Liuhui, ZHAN Liyun, HOU Yu, YU Weiwu, ZENG Yanru, DAI Wensheng. SSR analysis of genetic diversity of male Torreya grandis . Journal of Zhejiang A&F University, 2022, 39(2): 329-337. doi: 10.11833/j.issn.2095-0756.20210279
[5]	WANG Li, XU Hao, YU Yundong, ZHANG Haizhen, GAO Yanhui, SHEN Xiao, JIN Chenying, CHEN Yimin. Genetic diversity analysis of Hippeastrum rutilum cultivars based on SCoT markers . Journal of Zhejiang A&F University, 2020, 37(5): 930-938. doi: 10.11833/j.issn.2095-0756.20190614
[6]	WANG Luoyun, HE Caiyun, LUO Hongmei, ZHANG Jianguo, DUAN Aiguo, ZENG Yanfei. Genetic variation of 7 subspecies and 26 important cultivars in Hippophae rhamnoides . Journal of Zhejiang A&F University, 2019, 36(4): 670-677. doi: 10.11833/j.issn.2095-0756.2019.04.006
[7]	CHAI Xiaojuan, GUO Weilong, CHEN Hui, WANG Junrong, XIE Dezhi, JIN Shuihu. Genetic diversity of Juncus effusus germplasm by ISSR markers . Journal of Zhejiang A&F University, 2017, 34(3): 552-558. doi: 10.11833/j.issn.2095-0756.2017.03.022
[8]	YU Bao. Classification and management technology of trees in overcut forests of Larix gmelinii . Journal of Zhejiang A&F University, 2017, 34(2): 349-354. doi: 10.11833/j.issn.2095-0756.2017.02.020
[9]	LIU Yuejun, ZHANG Yuan, JIANG Yanfeng, LIU Jingjing. Genetic diversity of Polygonatum germplasm . Journal of Zhejiang A&F University, 2016, 33(6): 1085-1091. doi: 10.11833/j.issn.2095-0756.2016.06.023
[10]	BI Zhihong, WEI Minjing, LIU Yingying, YANG Chuanping, WEI Zhigang. Genetic parameters of Betula platyphylla provenances due to sample size . Journal of Zhejiang A&F University, 2016, 33(4): 564-570. doi: 10.11833/j.issn.2095-0756.2016.04.003
[11]	YU Bao, ZHANG Qiuliang, WANG Liming, SA Rula. Distribution pattern for regeneration of a middle to young cut-over Larix gmelinii forest . Journal of Zhejiang A&F University, 2015, 32(3): 346-352. doi: 10.11833/j.issn.2095-0756.2015.03.003
[12]	WU Yueyan, TAO Qiaojing, LI Bo, XU Danye. An optimal SRAP-PCR system of Rhododendron hybridum and its genetic diversity analysis with SRAP marker . Journal of Zhejiang A&F University, 2013, 30(6): 844-851. doi: 10.11833/j.issn.2095-0756.2013.06.007
[13]	GAO Yan-hui, FAN Min-liang, LUO Wen-jian, HUANG Hua-hong, TONG Zai-kang. RAPD analysis of genetic diversity for Pseudolarix amabilis： a critically endangered plant . Journal of Zhejiang A&F University, 2011, 28(5): 815-822. doi: 10.11833/j.issn.2095-0756.2011.05.023
[14]	FAN Fan-rong. Genetic diversity of endangered Artocarpus hypargyreus . Journal of Zhejiang A&F University, 2010, 27(2): 266-271. doi: 10.11833/j.issn.2095-0756.2010.02.017
[15]	LI Jie, XIONG Zhi, ZHANG Cheng-gang. Genetic diversity of Alnus nepalensis in Yunnan revealed by RAPD-PCR . Journal of Zhejiang A&F University, 2008, 25(1): 16-21.
[16]	WANG Zu-liang, DING Li-xia, ZHAO Ming-shui, CHENG Xiao-yuan, SHEN Qian. Genetic diversity of Ostrya rehderiana revealed by RAPD markers . Journal of Zhejiang A&F University, 2008, 25(3): 304-308.
[17]	CAO Fu-liang, HUA Zhe-bin, WANG Gui-bin, ZHANG Wang-xiang. Genetic diversity in wild populations of Ginkgo biloba using random amplified polymorphic DNA (RAPD) analysis . Journal of Zhejiang A&F University, 2008, 25(1): 22-27.
[18]	YANG Yi, ZHOU Zu-ji, ZHANG Li-qin, MA Liang-jin, LIN Xin-chun, GAO Jie. RAPD analysis of DNA polymorphism of parasitic Beauveria bassiana in Monochamus alternatus . Journal of Zhejiang A&F University, 2005, 22(3): 306-309.
[19]	TANG Juan-juan, FAN Yi-rong, ZHU Mu-yuan. Analysis of the genetic diversity of Pinus taiwanensis populations . Journal of Zhejiang A&F University, 2003, 20(1): 23-26.
[20]	HE Zhen-xiang, SHI Ji-sen, YIN Zeng-fang, CHEN Xiao-chou, YU Rong-zhuo. Detection of genetic markers associated with growth traits in Chinese fir . Journal of Zhejiang A&F University, 2000, 17(4): 350-354.

References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3557) PDF downloads(1725) Cited by()

Proportional views

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

1. State Key Laboratory of Forest Genetics and Tree Breeding，Northeast Forestry University，Harbin 150040，Heilongjiang，China

Corresponding author: ZHANG Han-guo

Received Date: 2011-03-28

Rev Recd Date: 2011-05-23

Publish Date: 2012-02-20

Key words:

Abstract: Random amplified polymorphic DNA （RAPD） markers associated with phenotypic characteristics were used to analyze the genetic variation of 270 individual samples from six natural populations of Larix gmelinii （Wuyiling，Ganhe，Kuduer，Nenjiang，Moerdaoga，and Aershan）. From 450 primers，23 clear RAPD primers were selected as their amplified bands with high polymorphism. The analysis included the gene diversity index，Shannons diversity index （SI），Neis gene diversity index，a cluster analysis，and a correlation analysis. Results showed 182 loci among a total of 186 were polymorphic or a percentage of polymorphism （PPB） of 97.9% having a range of 87.10%-94.09%. The gene diversity index was 0.374 3，and the genetic diversity within populations was 0.051 7 indicating that genetic variation was mainly within the population （86.19%）. Mean SI was 0.482 1，and the mean for Neis index was 0.322 6. The cluster analysis revealed six populations divided into three groups （the Xiaoxingan Mountain Range，northwest of the Daxingan Mountain Range，and south of the Daxingan Mountain Range） with a genetic distance of 0.09. Genetic distance was significantly and positively correlated with geographical distance（P＜0.05，r=0.768）. Growth traits of the four L. gmelinii populations were 10.25%-20.78% for height，14.93%-28.37% for diameter，and 35.03%-74.36% for volume coefficients of variation. Compared with the genetic variation of breeding populations，in natural populations PPB was 5.32% greater，mean of effective alleles numbered 1.13% greater，Neis index was 4.94% greater，SI was 5.68% greater，and the populations total genetic variation 8.78% greater.［Ch，3 fig. 6 tab. 10 ref.］

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

doi: 10.11833/j.issn.2095-0756.2012.01.022

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

doi: 10.11833/j.issn.2095-0756.2012.01.022

1. State Key Laboratory of Forest Genetics and Tree Breeding，Northeast Forestry University，Harbin 150040，Heilongjiang，China

Corresponding author: ZHANG Han-guo

HTML

Catalog

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

doi: 10.11833/j.issn.2095-0756.2012.01.022

Abstract

Relative Article

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Related

Proportional views

Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii

doi: 10.11833/j.issn.2095-0756.2012.01.022

1. State Key Laboratory of Forest Genetics and Tree Breeding，Northeast Forestry University，Harbin 150040，Heilongjiang，China

Corresponding author: ZHANG Han-guo

HTML

Catalog

Export File

Citation

Format

Content