Establishing and optimizing a SCoT-PCR system for <em>Lycoris</em>

JIANG Xiaofeng; GAO Yanhui; TONG Zaikang; HUANG Chunhong

doi:10.11833/j.issn.2095-0756.2013.03.023

Volume 30 Issue 3

May 2013

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JIANG Xiaofeng, GAO Yanhui, TONG Zaikang, HUANG Chunhong. Establishing and optimizing a SCoT-PCR system for Lycoris[J]. Journal of Zhejiang A&F University, 2013, 30(3): 444-452. doi: 10.11833/j.issn.2095-0756.2013.03.023

Citation:

JIANG Xiaofeng, GAO Yanhui, TONG Zaikang, HUANG Chunhong. Establishing and optimizing a SCoT-PCR system for Lycoris[J]. Journal of Zhejiang A&F University, 2013, 30(3): 444-452. doi: 10.11833/j.issn.2095-0756.2013.03.023

Establishing and optimizing a SCoT-PCR system for Lycoris

doi: 10.11833/j.issn.2095-0756.2013.03.023

1.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

More Information

Corresponding author: GAO Yanhui
Received Date: 2012-05-02
Rev Recd Date: 2012-09-21
Publish Date: 2013-06-20

Abstract

An optimal SCoT（start condon tagreted polymorphism）-PCR（polymerase chain reaction）amplification system was established to provide a new way of studying genetic diversity and genetic improvement in Lycoris. This system was optimized based on the L25（56）orthogonal experiment and single factor experiment. Results showed that the optimal reaction system （20 L）contained 2.0 mgL－1 DNA template, 0.125 molL－1 primers, 0.2 mmolL－1 dNTPs, 3.0 mmolL－1 Mg2+, and 1.0 16.67 nkat Taq DNA polymerase. 34 materials of Lycoris were randomly selected and the test was repeated 3 times. Using this optimal reaction system, stable and clear polymorphic bands were acquired. These favorable results could provide a scientific basis for further study of Lycoris.［Ch, 7 fig. 6 tab. 25 ref.］
- botany,
- Lycoris,
- SCoT（start condon tagreted polymorphism）,
- PCR（polymerase chain reaction）,
- orthogonal design,
- single factor experiment

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Establishing and optimizing a SCoT-PCR system for Lycoris

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Corresponding author: GAO Yanhui

Received Date: 2012-05-02

Rev Recd Date: 2012-09-21

Publish Date: 2013-06-20

Key words:

botany /
Lycoris /
SCoT（start condon tagreted polymorphism） /
PCR（polymerase chain reaction） /
orthogonal design /
single factor experiment

Abstract: An optimal SCoT（start condon tagreted polymorphism）-PCR（polymerase chain reaction）amplification system was established to provide a new way of studying genetic diversity and genetic improvement in Lycoris. This system was optimized based on the L25（56）orthogonal experiment and single factor experiment. Results showed that the optimal reaction system （20 L）contained 2.0 mgL－1 DNA template, 0.125 molL－1 primers, 0.2 mmolL－1 dNTPs, 3.0 mmolL－1 Mg2+, and 1.0 16.67 nkat Taq DNA polymerase. 34 materials of Lycoris were randomly selected and the test was repeated 3 times. Using this optimal reaction system, stable and clear polymorphic bands were acquired. These favorable results could provide a scientific basis for further study of Lycoris.［Ch, 7 fig. 6 tab. 25 ref.］

Establishing and optimizing a SCoT-PCR system for Lycoris

doi: 10.11833/j.issn.2095-0756.2013.03.023

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Establishing and optimizing a SCoT-PCR system for Lycoris

doi: 10.11833/j.issn.2095-0756.2013.03.023

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Corresponding author: GAO Yanhui

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Establishing and optimizing a SCoT-PCR system for Lycoris

doi: 10.11833/j.issn.2095-0756.2013.03.023

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通讯作者: 陈斌, bchen63@163.com

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Establishing and optimizing a SCoT-PCR system for Lycoris

doi: 10.11833/j.issn.2095-0756.2013.03.023

1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin’an 311300, Zhejiang, China

Corresponding author: GAO Yanhui

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